Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455 |
Resumo: | ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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Mini-percoll gradient may be used for frozen-thawed sperm selection in sheepsperm capacitationfrozen-thawed spermovinepercoll protocolsABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time.Universidade Federal de Minas Gerais, Escola de Veterinária2019-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-9807info:eu-repo/semantics/openAccessOlivares,C.C.S.Souza-Fabjan,J.M.G.Fonseca,J.F.Saraiva,H.F.R.A.Côrtes,L.R.Alfradique,V.A.P.Balaro,M.F.A.Oliveira,R.V.Brandão,F.Z.eng2019-06-03T00:00:00Zoai:scielo:S0102-09352019000200455Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2019-06-03T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
title |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
spellingShingle |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep Olivares,C.C.S. sperm capacitation frozen-thawed sperm ovine percoll protocols |
title_short |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
title_full |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
title_fullStr |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
title_full_unstemmed |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
title_sort |
Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep |
author |
Olivares,C.C.S. |
author_facet |
Olivares,C.C.S. Souza-Fabjan,J.M.G. Fonseca,J.F. Saraiva,H.F.R.A. Côrtes,L.R. Alfradique,V.A.P. Balaro,M.F.A. Oliveira,R.V. Brandão,F.Z. |
author_role |
author |
author2 |
Souza-Fabjan,J.M.G. Fonseca,J.F. Saraiva,H.F.R.A. Côrtes,L.R. Alfradique,V.A.P. Balaro,M.F.A. Oliveira,R.V. Brandão,F.Z. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Olivares,C.C.S. Souza-Fabjan,J.M.G. Fonseca,J.F. Saraiva,H.F.R.A. Côrtes,L.R. Alfradique,V.A.P. Balaro,M.F.A. Oliveira,R.V. Brandão,F.Z. |
dc.subject.por.fl_str_mv |
sperm capacitation frozen-thawed sperm ovine percoll protocols |
topic |
sperm capacitation frozen-thawed sperm ovine percoll protocols |
description |
ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4162-9807 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220892816801792 |