Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep

Detalhes bibliográficos
Autor(a) principal: Olivares,C.C.S.
Data de Publicação: 2019
Outros Autores: Souza-Fabjan,J.M.G., Fonseca,J.F., Saraiva,H.F.R.A., Côrtes,L.R., Alfradique,V.A.P., Balaro,M.F.A., Oliveira,R.V., Brandão,F.Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455
Resumo: ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time.
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spelling Mini-percoll gradient may be used for frozen-thawed sperm selection in sheepsperm capacitationfrozen-thawed spermovinepercoll protocolsABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time.Universidade Federal de Minas Gerais, Escola de Veterinária2019-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-9807info:eu-repo/semantics/openAccessOlivares,C.C.S.Souza-Fabjan,J.M.G.Fonseca,J.F.Saraiva,H.F.R.A.Côrtes,L.R.Alfradique,V.A.P.Balaro,M.F.A.Oliveira,R.V.Brandão,F.Z.eng2019-06-03T00:00:00Zoai:scielo:S0102-09352019000200455Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2019-06-03T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
title Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
spellingShingle Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
Olivares,C.C.S.
sperm capacitation
frozen-thawed sperm
ovine
percoll protocols
title_short Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
title_full Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
title_fullStr Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
title_full_unstemmed Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
title_sort Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
author Olivares,C.C.S.
author_facet Olivares,C.C.S.
Souza-Fabjan,J.M.G.
Fonseca,J.F.
Saraiva,H.F.R.A.
Côrtes,L.R.
Alfradique,V.A.P.
Balaro,M.F.A.
Oliveira,R.V.
Brandão,F.Z.
author_role author
author2 Souza-Fabjan,J.M.G.
Fonseca,J.F.
Saraiva,H.F.R.A.
Côrtes,L.R.
Alfradique,V.A.P.
Balaro,M.F.A.
Oliveira,R.V.
Brandão,F.Z.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Olivares,C.C.S.
Souza-Fabjan,J.M.G.
Fonseca,J.F.
Saraiva,H.F.R.A.
Côrtes,L.R.
Alfradique,V.A.P.
Balaro,M.F.A.
Oliveira,R.V.
Brandão,F.Z.
dc.subject.por.fl_str_mv sperm capacitation
frozen-thawed sperm
ovine
percoll protocols
topic sperm capacitation
frozen-thawed sperm
ovine
percoll protocols
description ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4162-9807
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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