Evaluation of potential biomarkers for the diagnosis and monitoring of Systemic Lupus Erythematosus using the Cytometric Beads Array (CBA)

• Autor(a) principal: Luan c v Alves
• Data de Publicação: 2019
• Outros Autores: Isabela Moreira Gondim, Luanne f Ferreira, Tania Mara Guimaraes, Vicente de Paula Coelho Peixoto Toledo, Maria Das Graças Carvalho, Fernanda Freire Campos, Edna Afonso Reis, Gilda Aparecida Ferreira, Debora Cerqueira Calderaro, Joana Starling de Carvalho, Paulo m Padua, Walter Batista Cicarini
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1016/j.cca.2019.08.033; http://hdl.handle.net/1843/56819
• topic: Systemic Lupus Erythematosus; Biomarkers; diagnostico; Lúpus Eritematoso Sistêmico; Biomarcadores

Background: Systemic Lupus Erythematosus (SLE) is an autoimmune, multisystemic disease. Currently diagnosis depends on complex criteria developed by the American College of Rheumatology. Moreover, the lack of specific biomarkers also challenges the diagnosis. Methods: Inflammatory biomarkers such as IL-8, IP-10, MIG, MIP-1α and RANTES were measured in serum samples from SLE patients and subjects in control groups (patients with other autoimmune diseases and healthy individuals). Forty-six SLE patients (22 patients with low activity, SLEDAI-2 K ≤ 4, 24 patients with moderate/ high activity, SLEDAI-2 K > 4), 42 patients with other autoimmune diseases (OAD group), and 8 healthy vo lunteers participated in this study. Results: MIG (p < .001) and RANTES (p < .001) concentrations in SLE patients and healthy controls, and IP-10 concentrations in SLE patients with different disease activities (low activity, p < .01, moderate/high activity, p < .05) differed significantly. IL-8 (p < .001) and MIP-1α (p < .001) concentrations in SLE patients differed from those in patients from the OAD group. IL-8 (p < .05), IP-10 (p < .01), MIG (p < .05), MIP-1α (p < .001), and RANTES (p < .05) were correlated with SLE activity; their concentrations in SLE patients with low and moderate/high activity differed significantly. Conclusions: Given the findings of this study, one can envision the possibility of future use of some of these cytokines to assist in the screening of SLE patients, or even in monitoring disease activity.