Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer

• Autor(a) principal: Léia Cardoso
• Data de Publicação: 2021
• Outros Autores: Mauro Aparecido de Sousa Xavier, Paula Ericson Guilherme Tambellini, Fábio Floriano Haesbaert, Anna Christina de Almeida, Vanessa de Andrade Royo, Alessandra Rejane Ericsson de Oliveira Xavier
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.34117/bjdv7n1-559; http://hdl.handle.net/1843/53424
• topic: Microrganismos; Enzimas -- Análise; Fontes termais; Ionização

The aim of this study was to isolate, identify, search for enzymes of biotechnological interest, trace the resistance profile to antimicrobials and verify the antagonistic activity of thermotolerant microorganisms isolated in thermal aquifer in Caldas Novas, Brazil. For this purpose, deep water samples were collected from three artesian wells, inoculated on nutrient agar and incubated at 37°C for 24 hours. The isolates were identified at species level by MALDI-TOF mass spectrometry. The identified species were screened for amylase, cellulase, gelatinase, caseinase, lipase, and pectinase enzymes according to standard methodologies. The profile of susceptibility bacteria to antimicrobials was performed by disc diffusion and the antagonist activity was evaluated against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 25923 bacteria. The obtained four isolates were identified as Bacillus pumilus, Bacillus subtilis and two as Bacillus megaterium. All isolates produced caseinase and gelatinase and they were sensitive to all antimicrobials tested. Bacillus pumilus, Bacillus subtilis and B. megaterium-3207 exhibited antagonistic activity against S. aureus. The antagonist activity against the S. aureus strain opens a perspective for future studies to identify secondary metabolites with potential antimicrobial activity.