Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.34117/bjdv7n1-559 http://hdl.handle.net/1843/53424 |
Resumo: | The aim of this study was to isolate, identify, search for enzymes of biotechnological interest, trace the resistance profile to antimicrobials and verify the antagonistic activity of thermotolerant microorganisms isolated in thermal aquifer in Caldas Novas, Brazil. For this purpose, deep water samples were collected from three artesian wells, inoculated on nutrient agar and incubated at 37°C for 24 hours. The isolates were identified at species level by MALDI-TOF mass spectrometry. The identified species were screened for amylase, cellulase, gelatinase, caseinase, lipase, and pectinase enzymes according to standard methodologies. The profile of susceptibility bacteria to antimicrobials was performed by disc diffusion and the antagonist activity was evaluated against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 25923 bacteria. The obtained four isolates were identified as Bacillus pumilus, Bacillus subtilis and two as Bacillus megaterium. All isolates produced caseinase and gelatinase and they were sensitive to all antimicrobials tested. Bacillus pumilus, Bacillus subtilis and B. megaterium-3207 exhibited antagonistic activity against S. aureus. The antagonist activity against the S. aureus strain opens a perspective for future studies to identify secondary metabolites with potential antimicrobial activity. |
id |
UFMG_407f0cc0977f2cc1263ab1b943177c15 |
---|---|
oai_identifier_str |
oai:repositorio.ufmg.br:1843/53424 |
network_acronym_str |
UFMG |
network_name_str |
Repositório Institucional da UFMG |
repository_id_str |
|
spelling |
2023-05-16T10:45:30Z2023-05-16T10:45:30Z20217182198236https://doi.org/10.34117/bjdv7n1-5592525-8761http://hdl.handle.net/1843/53424The aim of this study was to isolate, identify, search for enzymes of biotechnological interest, trace the resistance profile to antimicrobials and verify the antagonistic activity of thermotolerant microorganisms isolated in thermal aquifer in Caldas Novas, Brazil. For this purpose, deep water samples were collected from three artesian wells, inoculated on nutrient agar and incubated at 37°C for 24 hours. The isolates were identified at species level by MALDI-TOF mass spectrometry. The identified species were screened for amylase, cellulase, gelatinase, caseinase, lipase, and pectinase enzymes according to standard methodologies. The profile of susceptibility bacteria to antimicrobials was performed by disc diffusion and the antagonist activity was evaluated against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 25923 bacteria. The obtained four isolates were identified as Bacillus pumilus, Bacillus subtilis and two as Bacillus megaterium. All isolates produced caseinase and gelatinase and they were sensitive to all antimicrobials tested. Bacillus pumilus, Bacillus subtilis and B. megaterium-3207 exhibited antagonistic activity against S. aureus. The antagonist activity against the S. aureus strain opens a perspective for future studies to identify secondary metabolites with potential antimicrobial activity.O objetivo deste trabalho foi isolar identificar, rastrear enzimas de interesse biotecnológico, traçar o perfil de resistência a antimicrobianos e verificar a atividade antagonista de microrganismos termotolerantes isolados em aquífero termal em Caldas Novas, Brasil. Para tal foram coletadas amostras de águas profundas em três poços artesianos, inoculadas em ágar nutriente e incubadas a 37°C durante 24 horas. Os isolados foram identificados ao nível de espécie por espectrometria de massa MALDI-TOF. As espécies identificadas foram submetidas ao screening enzimático para amilase, celulase, gelatinase, caseinase, lipase e pectinase conforme metodologias padrão. O perfil de susceptibilidade das bactérias aos antimicrobianos foi realizado por disco difusão e a atividade antagonista foi avaliada frente à Escherichia coli ATCC 8739 e Staphilococcus aureus ATCC 25923. Foram obtidas quatro isolados identificados como Bacillus pumilus, Bacillus subtilis e dois Bacillus megaterium. Os isolados produziram caseinase e gelatinase e foram sensíveis a todos os antimicrobianos testados. Bacillus pumilus, Bacillus subtilis e B. megaterium 3207 exibiram atividade antagonista frente ao S. aureus. A atividade antagonista frente à cepa de S. aureus aqui encontrada abre uma perspectiva para futuros estudos de identificação de metabólitos secundários com potencial atividade antimicrobiana.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBrazilian Journal of DevelopmentMicrorganismosEnzimas -- AnáliseFontes termaisIonizaçãoIdentification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquiferIdentificação, caracterização enzimática, atividade antagonista e perfil de resistência a antimicrobianos de bactérias termotolerantes isoladas em aquífero termal brasileiroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/23559Léia CardosoMauro Aparecido de Sousa XavierPaula Ericson Guilherme TambelliniFábio Floriano HaesbaertAnna Christina de AlmeidaVanessa de Andrade RoyoAlessandra Rejane Ericsson de Oliveira Xavierinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/53424/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALIDENTI~1.PDFIDENTI~1.PDFapplication/pdf361714https://repositorio.ufmg.br/bitstream/1843/53424/2/IDENTI~1.PDF98ebc0ca1920bbb8c44acdae7e329ba8MD521843/534242023-05-16 17:27:11.569oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-05-16T20:27:11Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
dc.title.alternative.pt_BR.fl_str_mv |
Identificação, caracterização enzimática, atividade antagonista e perfil de resistência a antimicrobianos de bactérias termotolerantes isoladas em aquífero termal brasileiro |
title |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
spellingShingle |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer Léia Cardoso Microrganismos Enzimas -- Análise Fontes termais Ionização |
title_short |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
title_full |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
title_fullStr |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
title_full_unstemmed |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
title_sort |
Identification, characterization enzymatic antagonist activity and antimicrobial resistance profile of bacteria isolated from brazilian thermal aquifer |
author |
Léia Cardoso |
author_facet |
Léia Cardoso Mauro Aparecido de Sousa Xavier Paula Ericson Guilherme Tambellini Fábio Floriano Haesbaert Anna Christina de Almeida Vanessa de Andrade Royo Alessandra Rejane Ericsson de Oliveira Xavier |
author_role |
author |
author2 |
Mauro Aparecido de Sousa Xavier Paula Ericson Guilherme Tambellini Fábio Floriano Haesbaert Anna Christina de Almeida Vanessa de Andrade Royo Alessandra Rejane Ericsson de Oliveira Xavier |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Léia Cardoso Mauro Aparecido de Sousa Xavier Paula Ericson Guilherme Tambellini Fábio Floriano Haesbaert Anna Christina de Almeida Vanessa de Andrade Royo Alessandra Rejane Ericsson de Oliveira Xavier |
dc.subject.other.pt_BR.fl_str_mv |
Microrganismos Enzimas -- Análise Fontes termais Ionização |
topic |
Microrganismos Enzimas -- Análise Fontes termais Ionização |
description |
The aim of this study was to isolate, identify, search for enzymes of biotechnological interest, trace the resistance profile to antimicrobials and verify the antagonistic activity of thermotolerant microorganisms isolated in thermal aquifer in Caldas Novas, Brazil. For this purpose, deep water samples were collected from three artesian wells, inoculated on nutrient agar and incubated at 37°C for 24 hours. The isolates were identified at species level by MALDI-TOF mass spectrometry. The identified species were screened for amylase, cellulase, gelatinase, caseinase, lipase, and pectinase enzymes according to standard methodologies. The profile of susceptibility bacteria to antimicrobials was performed by disc diffusion and the antagonist activity was evaluated against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 25923 bacteria. The obtained four isolates were identified as Bacillus pumilus, Bacillus subtilis and two as Bacillus megaterium. All isolates produced caseinase and gelatinase and they were sensitive to all antimicrobials tested. Bacillus pumilus, Bacillus subtilis and B. megaterium-3207 exhibited antagonistic activity against S. aureus. The antagonist activity against the S. aureus strain opens a perspective for future studies to identify secondary metabolites with potential antimicrobial activity. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2023-05-16T10:45:30Z |
dc.date.available.fl_str_mv |
2023-05-16T10:45:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/53424 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.34117/bjdv7n1-559 |
dc.identifier.issn.pt_BR.fl_str_mv |
2525-8761 |
url |
https://doi.org/10.34117/bjdv7n1-559 http://hdl.handle.net/1843/53424 |
identifier_str_mv |
2525-8761 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Development |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
bitstream.url.fl_str_mv |
https://repositorio.ufmg.br/bitstream/1843/53424/1/License.txt https://repositorio.ufmg.br/bitstream/1843/53424/2/IDENTI~1.PDF |
bitstream.checksum.fl_str_mv |
fa505098d172de0bc8864fc1287ffe22 98ebc0ca1920bbb8c44acdae7e329ba8 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
|
_version_ |
1803589240267735040 |