Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite

Detalhes bibliográficos
Autor(a) principal: Vivianne Carvalho da Cunha Trajano
Data de Publicação: 2016
Outros Autores: Maria Esperanza Cortés Segura, Ruben Dario Sinisterra, Célia Regina Lanza, Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1016/j.msec.2016.03.103
http://hdl.handle.net/1843/49151
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2023-01-25T21:42:10Z2023-01-25T21:42:10Z2016-0764370375https://doi.org/10.1016/j.msec.2016.03.10323276053http://hdl.handle.net/1843/49151CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration. When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites. Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures. The DOX/βCD composite was prepared with a double mixing method. Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days. Composite structures were evaluated by scanning electron microscopy (SEM). Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week. AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days. These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period. SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts. Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic compositeengUniversidade Federal de Minas GeraisUFMGBrasilFAO - FACULDADE DE ODONTOLOGIAJournal of Materials Science and Chemical EngineeringDoxycyclinePolymersCyclodextrinsCompomersPeriodonticsOsteoblastsb-cyclodextrindoxycyclinePolymersCompositePeriodontal therapyOsteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga compositeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S0928493116302910?via%3DihubVivianne Carvalho da Cunha TrajanoMaria Esperanza Cortés SeguraRuben Dario SinisterraCélia Regina LanzaCostaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/49151/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALOsteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite.pdfOsteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite.pdfapplication/pdf950667https://repositorio.ufmg.br/bitstream/1843/49151/2/Osteogenic%20activity%20of%20cyclodextrin-encapsulated%20doxycycline%20in%20a%20calcium%20phosphate%20pcl%20and%20plga%20composite.pdf20fa4a56c7f0870828157d95e6f1015fMD521843/491512023-01-25 18:42:10.932oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-01-25T21:42:10Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
title Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
spellingShingle Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
Vivianne Carvalho da Cunha Trajano
b-cyclodextrin
doxycycline
Polymers
Composite
Periodontal therapy
Doxycycline
Polymers
Cyclodextrins
Compomers
Periodontics
Osteoblasts
title_short Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
title_full Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
title_fullStr Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
title_full_unstemmed Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
title_sort Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
author Vivianne Carvalho da Cunha Trajano
author_facet Vivianne Carvalho da Cunha Trajano
Maria Esperanza Cortés Segura
Ruben Dario Sinisterra
Célia Regina Lanza
Costa
author_role author
author2 Maria Esperanza Cortés Segura
Ruben Dario Sinisterra
Célia Regina Lanza
Costa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vivianne Carvalho da Cunha Trajano
Maria Esperanza Cortés Segura
Ruben Dario Sinisterra
Célia Regina Lanza
Costa
dc.subject.por.fl_str_mv b-cyclodextrin
doxycycline
Polymers
Composite
Periodontal therapy
topic b-cyclodextrin
doxycycline
Polymers
Composite
Periodontal therapy
Doxycycline
Polymers
Cyclodextrins
Compomers
Periodontics
Osteoblasts
dc.subject.other.pt_BR.fl_str_mv Doxycycline
Polymers
Cyclodextrins
Compomers
Periodontics
Osteoblasts
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2016
dc.date.issued.fl_str_mv 2016-07
dc.date.accessioned.fl_str_mv 2023-01-25T21:42:10Z
dc.date.available.fl_str_mv 2023-01-25T21:42:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/49151
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1016/j.msec.2016.03.103
dc.identifier.issn.pt_BR.fl_str_mv 23276053
url https://doi.org/10.1016/j.msec.2016.03.103
http://hdl.handle.net/1843/49151
identifier_str_mv 23276053
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Journal of Materials Science and Chemical Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv FAO - FACULDADE DE ODONTOLOGIA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/49151/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/49151/2/Osteogenic%20activity%20of%20cyclodextrin-encapsulated%20doxycycline%20in%20a%20calcium%20phosphate%20pcl%20and%20plga%20composite.pdf
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