Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/1518-8345.3101.3211 http://hdl.handle.net/1843/55800 https://orcid.org/0000-0002-9272-641X https://orcid.org/0000-0002-9859-5895 https://orcid.org/0000-0001-6519-8490 https://orcid.org/0000-0002-7462-767X https://orcid.org/0000-0002-4821-6068 |
Resumo: | Objective: to evaluate the potential contamination of enzymatic detergent from its reuse and to identify the microbiological profile in the solution used to clean gastrointestinal endoscopic devices. Method: cross-sectional study based on microbiological analysis of 76 aliquots of 19 different enzymatic detergent solutions used to clean endoscopic devices. The aliquots were homogenized, subjected to Millipore® 0.45 µm membrane filtration and the presumptive identification of microorganisms was performed by biochemical-physiological methods according to previously established specific bacterial groups that are of clinical and epidemiological relevance. Results: the mean values, as well as the standard deviation and the median, of the enzymatic detergent microbial load increased as the solution was reused. There was a significant difference between the means of after first use and after fifth reuse. A total of 97 microorganisms were identified, with predominance of the coagulase-negative Staphylococcus, Pseudomonas spp., Klebsiella spp., Enterobacter spp. genus, and Escherichia coli species. Conclusion: the reuse of the enzymatic detergent solution is a risk to the safe processing of endoscopic devices, evidenced by its contamination with pathogenic potential microorganisms, since the enzymatic detergent has no bactericidal property and can contribute as an important source for outbreaks in patients under such procedures. |
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2023-07-04T22:49:21Z2023-07-04T22:49:21Z201927https://doi.org/10.1590/1518-8345.3101.32111518-8345http://hdl.handle.net/1843/55800https://orcid.org/0000-0002-9272-641Xhttps://orcid.org/0000-0002-9859-5895https://orcid.org/0000-0001-6519-8490https://orcid.org/0000-0002-7462-767Xhttps://orcid.org/0000-0002-4821-6068Objective: to evaluate the potential contamination of enzymatic detergent from its reuse and to identify the microbiological profile in the solution used to clean gastrointestinal endoscopic devices. Method: cross-sectional study based on microbiological analysis of 76 aliquots of 19 different enzymatic detergent solutions used to clean endoscopic devices. The aliquots were homogenized, subjected to Millipore® 0.45 µm membrane filtration and the presumptive identification of microorganisms was performed by biochemical-physiological methods according to previously established specific bacterial groups that are of clinical and epidemiological relevance. Results: the mean values, as well as the standard deviation and the median, of the enzymatic detergent microbial load increased as the solution was reused. There was a significant difference between the means of after first use and after fifth reuse. A total of 97 microorganisms were identified, with predominance of the coagulase-negative Staphylococcus, Pseudomonas spp., Klebsiella spp., Enterobacter spp. genus, and Escherichia coli species. Conclusion: the reuse of the enzymatic detergent solution is a risk to the safe processing of endoscopic devices, evidenced by its contamination with pathogenic potential microorganisms, since the enzymatic detergent has no bactericidal property and can contribute as an important source for outbreaks in patients under such procedures.Objetivo: avaliar a potencial contaminação do detergente enzimático a partir de seu reuso e identificar o perfil microbiológico na solução utilizada na limpeza de aparelhos endoscópicos gastrointestinais. Método: estudo transversal realizado a partir da análise microbiológica de 76 alíquotas de 19 diferentes soluções de detergente enzimático utilizadas na limpeza de aparelhos endoscópicos. As alíquotas foram homogeneizadas, submetidas a filtração em membrana Millipore® 0,45μm e a identificação presuntiva dos microrganismos foi realizada por métodos bioquímico-fisiológico conforme grupos bacterianos específicos previamente estabelecidos e que apresentam importância clínica e epidemiológica. Resultados: Os valores médios, assim como o desvio-padrão e a mediana, da carga microbiana do detergente enzimático foram crescentes à medida que a solução foi reutilizada. Houve diferença significativa entre as médias de após primeiro uso e após quinto reuso. Foram identificados 97 microrganismos, com predomínio dos gêneros Staphylococcus coagulase negativa, Pseudomonasspp., Klebsiella spp., Enterobacter spp., e da espécie Escherichia coli. Conclusão: O reuso da solução de detergente enzimático constitui um risco ao processamento seguro dos aparelhos endoscópios, evidenciado pela sua contaminação com microrganismos que apresentam potencial patogênico, uma vez que o detergente enzimático não possui propriedade bactericida e pode contribuir como fonte importante para surtos em pacientes submetidos a tais procedimentos.engUniversidade Federal de Minas GeraisUFMGBrasilENF - DEPARTAMENTO DE ENFERMAGEM BÁSICAICB - DEPARTAMENTO DE MICROBIOLOGIAICB - INSTITUTO DE CIÊNCIAS BIOLOGICASRevista Latino-Americana de EnfermagemSegurança do PacienteEndoscópiosDetergentesSaneantesMicrobiologiaControle de InfecçõesPatient safetyEndoscopesDetergentsSanitizing productsMicrobiologyInfection controlEnzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmissionReutilização do detergente enzimático no processamento de gastroscópios: uma potencial fonte de transmissão de microrganismosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/rlae/a/HLvtRPBKRCCwmF3zz8JZVsw/abstract/?lang=enMaria Letícia de MirandamatiNatalia Rocha GuimarãesPaula Prazeres MagalhãesLuiz de Macêdo FariasAdriana Cristina de Oliveiraapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINALEnzymatic detergent reuse in gastroscope processing a potential source of microorganism transmission.pdfEnzymatic detergent reuse in gastroscope processing a potential source of microorganism transmission.pdfapplication/pdf5712843https://repositorio.ufmg.br/bitstream/1843/55800/2/Enzymatic%20detergent%20reuse%20in%20gastroscope%20processing%20a%20potential%20source%20of%20microorganism%20transmission.pdf08e82143b3d56c41e9ef660605bf8ba9MD52LICENSELicense.txtLicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
dc.title.alternative.pt_BR.fl_str_mv |
Reutilização do detergente enzimático no processamento de gastroscópios: uma potencial fonte de transmissão de microrganismos |
title |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
spellingShingle |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission Maria Letícia de Mirandamati Patient safety Endoscopes Detergents Sanitizing products Microbiology Infection control Segurança do Paciente Endoscópios Detergentes Saneantes Microbiologia Controle de Infecções |
title_short |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
title_full |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
title_fullStr |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
title_full_unstemmed |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
title_sort |
Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission |
author |
Maria Letícia de Mirandamati |
author_facet |
Maria Letícia de Mirandamati Natalia Rocha Guimarães Paula Prazeres Magalhães Luiz de Macêdo Farias Adriana Cristina de Oliveira |
author_role |
author |
author2 |
Natalia Rocha Guimarães Paula Prazeres Magalhães Luiz de Macêdo Farias Adriana Cristina de Oliveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Maria Letícia de Mirandamati Natalia Rocha Guimarães Paula Prazeres Magalhães Luiz de Macêdo Farias Adriana Cristina de Oliveira |
dc.subject.por.fl_str_mv |
Patient safety Endoscopes Detergents Sanitizing products Microbiology Infection control |
topic |
Patient safety Endoscopes Detergents Sanitizing products Microbiology Infection control Segurança do Paciente Endoscópios Detergentes Saneantes Microbiologia Controle de Infecções |
dc.subject.other.pt_BR.fl_str_mv |
Segurança do Paciente Endoscópios Detergentes Saneantes Microbiologia Controle de Infecções |
description |
Objective: to evaluate the potential contamination of enzymatic detergent from its reuse and to identify the microbiological profile in the solution used to clean gastrointestinal endoscopic devices. Method: cross-sectional study based on microbiological analysis of 76 aliquots of 19 different enzymatic detergent solutions used to clean endoscopic devices. The aliquots were homogenized, subjected to Millipore® 0.45 µm membrane filtration and the presumptive identification of microorganisms was performed by biochemical-physiological methods according to previously established specific bacterial groups that are of clinical and epidemiological relevance. Results: the mean values, as well as the standard deviation and the median, of the enzymatic detergent microbial load increased as the solution was reused. There was a significant difference between the means of after first use and after fifth reuse. A total of 97 microorganisms were identified, with predominance of the coagulase-negative Staphylococcus, Pseudomonas spp., Klebsiella spp., Enterobacter spp. genus, and Escherichia coli species. Conclusion: the reuse of the enzymatic detergent solution is a risk to the safe processing of endoscopic devices, evidenced by its contamination with pathogenic potential microorganisms, since the enzymatic detergent has no bactericidal property and can contribute as an important source for outbreaks in patients under such procedures. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2023-07-04T22:49:21Z |
dc.date.available.fl_str_mv |
2023-07-04T22:49:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/55800 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/1518-8345.3101.3211 |
dc.identifier.issn.pt_BR.fl_str_mv |
1518-8345 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-9272-641X https://orcid.org/0000-0002-9859-5895 https://orcid.org/0000-0001-6519-8490 https://orcid.org/0000-0002-7462-767X https://orcid.org/0000-0002-4821-6068 |
url |
https://doi.org/10.1590/1518-8345.3101.3211 http://hdl.handle.net/1843/55800 https://orcid.org/0000-0002-9272-641X https://orcid.org/0000-0002-9859-5895 https://orcid.org/0000-0001-6519-8490 https://orcid.org/0000-0002-7462-767X https://orcid.org/0000-0002-4821-6068 |
identifier_str_mv |
1518-8345 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Revista Latino-Americana de Enfermagem |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ENF - DEPARTAMENTO DE ENFERMAGEM BÁSICA ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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