Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental

Detalhes bibliográficos
Autor(a) principal: Pereira, Nhaára da Vila
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMT
Texto Completo: http://ri.ufmt.br/handle/1/4601
Resumo: Historically the popular use of natural products for various purposes has been reported in the most varied contexts of human history. Highlighting the importance of ethnopharmacology is fundamental from the understanding that empirical knowledge and local knowledge practices are inseparable tools of cultural values from different social backgrounds and thus constitute productive resources for the conservation of nature and self-management of natural resources. From this knowledge, several plants have been used in traditional medicine for therapeutic purposes to treat and prevent various diseases. The extracts obtained from these vegetables contain a complex mixture of several components that can synergistically contribute to biological or therapeutic activity. This study aimed to carry out an ethnopharmacological survey of medicinal plants and to evaluate the antioxidant activity and cytotoxic potential in vitro. The study was conducted in the region of Mirante da Serra-RO, in the rural area of Lh. 58. A pretest was applied to choose and evaluate the techniques to be used with the local population to better adjust to the research purposes. The information collection consisted of the application of semistructured interviews with closed and open questions, and direct observation and for the data collection the snowball sampling technique was used. In order to analyze the association between the use of medicinal species and nosological categories, the International Statistical Classification of Diseases and Related Health Problems (ICD-10) was used. For the treatment of ethnopharmacological data we used the calculations of Fidelity Level (NF), Correction Factor (HR), relative frequency of use of each cited species (Pcup%) and Jaccard similarity. The species Adenocalymma bracteolatum, Stachytarpheta cayennensis, Piper hispidinervum, Pfaffia glomerata and Petiveria alliaceae, were highlighted for their fidelity level and were submitted for in vitro evaluation of cytotoxic and antioxidant potential. In vitro cytotoxic potential was evaluated by the Alamar Blue method using CHO-K1 non-metabolizing cells and the antioxidant activity was performed by the 1,1-diphenyl-2-picrilhydraz (DPPH) method. New discoveries are continually being made with plant-derived products. Several plant extracts are object of study in numerous researches that evaluate antioxidant action cytotoxicity, antiviral antifungal, among others. In this sense, the performance of the techniques and methods evaluated in view of the therapeutic potential for traditional use points to a promising future.
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spelling Bioprospecção etnofarmacológica de plantas medicinais na Amazônia OcidentalCitotoxicidadeCHO-K1Compostos bioativosAntioxidanteDPPHEtnofarmacologiaSnowball samplingCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALCytotoxicityCHO-K1Bioactive compoundsAntioxidantDPPHEthnopharmacologySnowball samplingHistorically the popular use of natural products for various purposes has been reported in the most varied contexts of human history. Highlighting the importance of ethnopharmacology is fundamental from the understanding that empirical knowledge and local knowledge practices are inseparable tools of cultural values from different social backgrounds and thus constitute productive resources for the conservation of nature and self-management of natural resources. From this knowledge, several plants have been used in traditional medicine for therapeutic purposes to treat and prevent various diseases. The extracts obtained from these vegetables contain a complex mixture of several components that can synergistically contribute to biological or therapeutic activity. This study aimed to carry out an ethnopharmacological survey of medicinal plants and to evaluate the antioxidant activity and cytotoxic potential in vitro. The study was conducted in the region of Mirante da Serra-RO, in the rural area of Lh. 58. A pretest was applied to choose and evaluate the techniques to be used with the local population to better adjust to the research purposes. The information collection consisted of the application of semistructured interviews with closed and open questions, and direct observation and for the data collection the snowball sampling technique was used. In order to analyze the association between the use of medicinal species and nosological categories, the International Statistical Classification of Diseases and Related Health Problems (ICD-10) was used. For the treatment of ethnopharmacological data we used the calculations of Fidelity Level (NF), Correction Factor (HR), relative frequency of use of each cited species (Pcup%) and Jaccard similarity. The species Adenocalymma bracteolatum, Stachytarpheta cayennensis, Piper hispidinervum, Pfaffia glomerata and Petiveria alliaceae, were highlighted for their fidelity level and were submitted for in vitro evaluation of cytotoxic and antioxidant potential. In vitro cytotoxic potential was evaluated by the Alamar Blue method using CHO-K1 non-metabolizing cells and the antioxidant activity was performed by the 1,1-diphenyl-2-picrilhydraz (DPPH) method. New discoveries are continually being made with plant-derived products. Several plant extracts are object of study in numerous researches that evaluate antioxidant action cytotoxicity, antiviral antifungal, among others. In this sense, the performance of the techniques and methods evaluated in view of the therapeutic potential for traditional use points to a promising future.CAPESHistoricamente o uso popular de produtos naturais para as mais diversas finalidades é relatado nos mais variados contextos da história da humanidade. Destacar a importância da Etnofarmacologica é fundamental a partir do entendimento de que os saberes empíricos e as práticas de saber local são ferramentas indissociáveis dos valores culturais de diferentes formações sociais a assim constituindo recursos produtivos para a conservação da natureza e autogestão dos recursos naturais. Oriundas deste conhecimento, diversas plantas tem sido utilizadas na medicina tradicional com propósito terapêutico para tratamento e prevenção de diversas doenças. Os extratos obtidos a partir destes vegetais contém uma mistura complexa de diversos componentes que de maneira sinérgica podem contribuir na atividade biológica ou terapêutica. O presente estudo teve como objetivo realizar um levantamento etnofarmacológico das plantas medicinais e avaliar a atividade antioxidante e o potencial citotóxico in vitro. O estudo foi realizado na região de Mirante da Serra- RO, na área rural da Linha (Lh) 58. Aplicouse um pré-teste para escolher e avaliar as técnicas a serem utilizadas junto à população local para melhor ajustamento às finalidades da pesquisa. A coleta de informações consistiu na aplicação de entrevistas semiestruturadas com perguntas fechadas e abertas, e observação direta e para a coleta de dados foi utilizada a técnica snowball sampling. Com o intuito de analisar a associação do uso de espécies medicinais com as categorias nosológicas, utilizou-se a Classificação Estatística Internacional de Doenças e Problemas Relacionados à Saúde (CID-10). Para o tratamento dos dados etnofarmacológicos foram utilizados os cálculos de Nível de Fidelidade (NF), Fator de Correção (FC), frequência relativa do uso de cada espécie citada (Pcup%) e similaridade de Jaccard. As espécies Adenocalymma bracteolatum, Stachytarpheta cayennensis, Piper hispidinervum, Pfaffia glomerata e Petiveria alliaceae, obtiveram destaque quanto ao nível de fidelidade, e foram submetidas á avaliação in vitro do potencial citotóxico e antioxidante. O potencial citotóxico in vitro foi avaliado pelo método de Alamar Blue, utilizando células não metabolizadoras CHO-K1 e a atividade antioxidante foram realizadas pelo método 1,1-difenil-2-picril-hidrazil (DPPH). Novas descobertas vêm sendo feitas continuamente com produtos derivados das plantas. Diversos extratos vegetais são objetos de estudo em inúmeras pesquisas que avaliam ação antioxidante citotoxicidade, antiviral antifúngica, entre outras. Nesse sentido, o desempenho das técnicas e métodos avaliados frente às potencialidades terapêuticas com vistas ao uso tradicional aponta para um futuro promissor.Universidade Federal de Mato GrossoBrasilFaculdade de Engenharia Florestal (FENF)UFMT CUC - CuiabáPrograma de Pós-Graduação em Ciências Florestais e AmbientaisPasa, Maria CoretteSalatino, Maria Luizahttp://lattes.cnpq.br/6864401803361183http://lattes.cnpq.br/6935789833701375Pasa, Maria Corette303.265.080-15http://lattes.cnpq.br/6935789833701375Guarim Neto, Germano079.167.521-15http://lattes.cnpq.br/8969553047461469303.265.080-15.Valentini, Carla Maria Abido432.459.271-34http://lattes.cnpq.br/6189350328079584Pereira, Nhaára da Vila2023-08-08T16:35:23Z2020-10-212023-08-08T16:35:23Z2020-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPEREIRA, Nhaára da Vila. Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental. 2020. 123 f. Dissertação (Mestrado em Ciências Florestais e Ambientais) - Universidade Federal de Mato Grosso, Faculdade de Engenharia Florestal, Cuiabá, 2020.http://ri.ufmt.br/handle/1/4601porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2023-08-11T07:03:34Zoai:localhost:1/4601Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2023-08-11T07:03:34Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
title Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
spellingShingle Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
Pereira, Nhaára da Vila
Citotoxicidade
CHO-K1
Compostos bioativos
Antioxidante
DPPH
Etnofarmacologia
Snowball sampling
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
Cytotoxicity
CHO-K1
Bioactive compounds
Antioxidant
DPPH
Ethnopharmacology
Snowball sampling
title_short Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
title_full Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
title_fullStr Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
title_full_unstemmed Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
title_sort Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental
author Pereira, Nhaára da Vila
author_facet Pereira, Nhaára da Vila
author_role author
dc.contributor.none.fl_str_mv Pasa, Maria Corette
Salatino, Maria Luiza
http://lattes.cnpq.br/6864401803361183
http://lattes.cnpq.br/6935789833701375
Pasa, Maria Corette
303.265.080-15
http://lattes.cnpq.br/6935789833701375
Guarim Neto, Germano
079.167.521-15
http://lattes.cnpq.br/8969553047461469
303.265.080-15
.
Valentini, Carla Maria Abido
432.459.271-34
http://lattes.cnpq.br/6189350328079584
dc.contributor.author.fl_str_mv Pereira, Nhaára da Vila
dc.subject.por.fl_str_mv Citotoxicidade
CHO-K1
Compostos bioativos
Antioxidante
DPPH
Etnofarmacologia
Snowball sampling
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
Cytotoxicity
CHO-K1
Bioactive compounds
Antioxidant
DPPH
Ethnopharmacology
Snowball sampling
topic Citotoxicidade
CHO-K1
Compostos bioativos
Antioxidante
DPPH
Etnofarmacologia
Snowball sampling
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
Cytotoxicity
CHO-K1
Bioactive compounds
Antioxidant
DPPH
Ethnopharmacology
Snowball sampling
description Historically the popular use of natural products for various purposes has been reported in the most varied contexts of human history. Highlighting the importance of ethnopharmacology is fundamental from the understanding that empirical knowledge and local knowledge practices are inseparable tools of cultural values from different social backgrounds and thus constitute productive resources for the conservation of nature and self-management of natural resources. From this knowledge, several plants have been used in traditional medicine for therapeutic purposes to treat and prevent various diseases. The extracts obtained from these vegetables contain a complex mixture of several components that can synergistically contribute to biological or therapeutic activity. This study aimed to carry out an ethnopharmacological survey of medicinal plants and to evaluate the antioxidant activity and cytotoxic potential in vitro. The study was conducted in the region of Mirante da Serra-RO, in the rural area of Lh. 58. A pretest was applied to choose and evaluate the techniques to be used with the local population to better adjust to the research purposes. The information collection consisted of the application of semistructured interviews with closed and open questions, and direct observation and for the data collection the snowball sampling technique was used. In order to analyze the association between the use of medicinal species and nosological categories, the International Statistical Classification of Diseases and Related Health Problems (ICD-10) was used. For the treatment of ethnopharmacological data we used the calculations of Fidelity Level (NF), Correction Factor (HR), relative frequency of use of each cited species (Pcup%) and Jaccard similarity. The species Adenocalymma bracteolatum, Stachytarpheta cayennensis, Piper hispidinervum, Pfaffia glomerata and Petiveria alliaceae, were highlighted for their fidelity level and were submitted for in vitro evaluation of cytotoxic and antioxidant potential. In vitro cytotoxic potential was evaluated by the Alamar Blue method using CHO-K1 non-metabolizing cells and the antioxidant activity was performed by the 1,1-diphenyl-2-picrilhydraz (DPPH) method. New discoveries are continually being made with plant-derived products. Several plant extracts are object of study in numerous researches that evaluate antioxidant action cytotoxicity, antiviral antifungal, among others. In this sense, the performance of the techniques and methods evaluated in view of the therapeutic potential for traditional use points to a promising future.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-21
2020-02-28
2023-08-08T16:35:23Z
2023-08-08T16:35:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PEREIRA, Nhaára da Vila. Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental. 2020. 123 f. Dissertação (Mestrado em Ciências Florestais e Ambientais) - Universidade Federal de Mato Grosso, Faculdade de Engenharia Florestal, Cuiabá, 2020.
http://ri.ufmt.br/handle/1/4601
identifier_str_mv PEREIRA, Nhaára da Vila. Bioprospecção etnofarmacológica de plantas medicinais na Amazônia Ocidental. 2020. 123 f. Dissertação (Mestrado em Ciências Florestais e Ambientais) - Universidade Federal de Mato Grosso, Faculdade de Engenharia Florestal, Cuiabá, 2020.
url http://ri.ufmt.br/handle/1/4601
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Faculdade de Engenharia Florestal (FENF)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ciências Florestais e Ambientais
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Faculdade de Engenharia Florestal (FENF)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ciências Florestais e Ambientais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Repositório Institucional da UFMT
collection Repositório Institucional da UFMT
repository.name.fl_str_mv Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv jordanbiblio@gmail.com
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