Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMT |
Texto Completo: | http://ri.ufmt.br/handle/1/2117 |
Resumo: | The Brazilian biodiversity has been studied by several researches that are looking for determine the effect of plant extracts against plant pathogens, as an alternative to the use of chemicals products. Face to this, the present research aimed to study part of the brasilian flora in order to verify the effect of extracts of sibipiruna leaves (Caesalpinia peltophoroides) in three Colletotrichum sp using two methodologies: evaluate the fungitoxic effect of different extracts concentrations of C. peltophoroides and white pitch (Protium heptaphyllum) against Colletotrichum truncatum, Colletotrichum sp., Fusarium equiseti, F. prolifetarum and F. solani. First, tests were performed with plant pathogens Colletotrichum sp. and C. truncatum isolated of soy beans from three different locations, through the methods of disk diffusion and spreading. It was used the ethereal fraction of leaves of C. peltophoroides and ethanolic extract of leaves of P. heptaphyllum against isolates of C. truncatum, Colletotrichum sp., F. equiseti, F. solani and F. prolifetarum tested in various concentrations for disk diffusion method. It was found the percentages in inhibition of mycelial growth, mycelial growth rate index, inhibition of sporulation, germination, and training appressoria. The ethanolic extract from the leaves of C. peltophoroides shows antifungal activity against three kinds of Colletotrichum, it was observed that the reduced speed of mycelial growth, which was similar to the fungicide. Inhibit sporulation, in some concentrations, above 60%; and the appressorium formation to 92.30%, proving to be effective. The methodology scattering when compared to disk diffusion showed better results for the evaluation of antifungal activity in the inhibition of sporulation. The C. peltophoroides extract was effective in all tested variables, with satisfactory results in at least one of the isolated and evaluated species. The C. peltophoroides extract has antifungal activity and can be an efficient and environmentally friendly alternative for the control of plant pathogens. The P. heptaphyllum extract did not inhibit mycelial growth and sporulation of the evaluated plant pathogens; however, inhibit the germination and the formation of appressoria, especially species of Fusarium. From the results obtained in this study, one can conclude that the ethanolic extracts of leaves of C. peltophoroides and P. heptaphyllum have antimicrobial potential on some of the variables tested. Therefore, further studies should be performed to confirm the in vivo bioactivity of antifungal in vivo species of C. peltophoroides and P. heptaphyllum and identify specific bioactive compounds and their mode of action, so that they can eventually be used as an alternative to fungicides. |
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Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenosAgrotóxicosMedidas alternativasCompostos bioativosAtividade antifúngicaCNPQ::OUTROS::CIENCIASAgrochemicalsAlternative measuresBioactive compoundsAntifungal activityThe Brazilian biodiversity has been studied by several researches that are looking for determine the effect of plant extracts against plant pathogens, as an alternative to the use of chemicals products. Face to this, the present research aimed to study part of the brasilian flora in order to verify the effect of extracts of sibipiruna leaves (Caesalpinia peltophoroides) in three Colletotrichum sp using two methodologies: evaluate the fungitoxic effect of different extracts concentrations of C. peltophoroides and white pitch (Protium heptaphyllum) against Colletotrichum truncatum, Colletotrichum sp., Fusarium equiseti, F. prolifetarum and F. solani. First, tests were performed with plant pathogens Colletotrichum sp. and C. truncatum isolated of soy beans from three different locations, through the methods of disk diffusion and spreading. It was used the ethereal fraction of leaves of C. peltophoroides and ethanolic extract of leaves of P. heptaphyllum against isolates of C. truncatum, Colletotrichum sp., F. equiseti, F. solani and F. prolifetarum tested in various concentrations for disk diffusion method. It was found the percentages in inhibition of mycelial growth, mycelial growth rate index, inhibition of sporulation, germination, and training appressoria. The ethanolic extract from the leaves of C. peltophoroides shows antifungal activity against three kinds of Colletotrichum, it was observed that the reduced speed of mycelial growth, which was similar to the fungicide. Inhibit sporulation, in some concentrations, above 60%; and the appressorium formation to 92.30%, proving to be effective. The methodology scattering when compared to disk diffusion showed better results for the evaluation of antifungal activity in the inhibition of sporulation. The C. peltophoroides extract was effective in all tested variables, with satisfactory results in at least one of the isolated and evaluated species. The C. peltophoroides extract has antifungal activity and can be an efficient and environmentally friendly alternative for the control of plant pathogens. The P. heptaphyllum extract did not inhibit mycelial growth and sporulation of the evaluated plant pathogens; however, inhibit the germination and the formation of appressoria, especially species of Fusarium. From the results obtained in this study, one can conclude that the ethanolic extracts of leaves of C. peltophoroides and P. heptaphyllum have antimicrobial potential on some of the variables tested. Therefore, further studies should be performed to confirm the in vivo bioactivity of antifungal in vivo species of C. peltophoroides and P. heptaphyllum and identify specific bioactive compounds and their mode of action, so that they can eventually be used as an alternative to fungicides.Diversas pesquisas têm estudado a biodiversidade brasileira e buscado verificar o efeito de extratos vegetais contra fitopatógenos, como alternativa ao uso de produtos químicos. Diante disto, a presente pesquisa buscou estudar parte da flora brasileira com o objetivo de verificar o efeito dos extratos das folhas de sibipiruna (Caesalpinia peltophoroides) em três isolados de Colletotrichum sp., utilizando duas metodologias; e, avaliar o efeito fungitóxico de diferentes concentrações dos extratos de C. peltophoroides e breu branco (Protium heptaphyllum) contra isolados de Colletotrichum truncatum, Colletotrichum sp., Fusarium equiseti, F. prolifetarum e F. solani. Primeiramente, os ensaios foram realizados com os fitopatógenos Colletotrichum sp. e C. truncatum isolados de soja de três diferentes localidades, através dos métodos de difusão em disco e espalhamento. Utilizou-se a fração etérea das folhas de C. peltophoroides e o extrato etanólico das folhas de P. heptaphyllum contra isolados de C. truncatum, Colletotrichum sp., F. equiseti, F. prolifetarum e F. solani testados em várias concentrações, pela metodologia de difusão em disco. Verificou-se a porcentagem de inibição de crescimento micelial, índice de velocidade de crescimento micelial, porcentagem de inibição de esporulação, germinação, e formação de apressórios. O extrato etanólico das folhas de C. peltophoroides apresenta atividade antifúngica contra três isolados de Colletotrichum, visto que, reduziu a velocidade do crescimento micelial, sendo similar ao fungicida. Inibiu a esporulação, em algumas concentrações, acima de 60%; e, a formação de apressórios em até 92,30%, demonstrando ser eficaz. A metodologia de espalhamento quando comparada à de disco-difusão apresentou melhores resultados para avaliação da atividade antifúngica na inibição da esporulação. O extrato de C. peltophoroides foi eficaz em todas as variáveis testadas, com resultados satisfatórios em pelo menos um dos isolados e espécies avaliadas. Extrato de C. peltophoroides apresenta atividade antifúngica e pode ser uma alternativa eficiente e ecológica no controle de fitopatógenos. O extrato de P. heptaphyllum não inibiu o crescimento micelial e esporulação dos fitopatógenos avaliados; no entanto, inibiu a germinação e formação de apressórios, principalmente das espécies de Fusarium. A partir dos resultados obtidos nesta pesquisa, pode-se concluir que os extratos etanólicos das folhas de C. peltophoroides e P. heptaphyllum apresentam potencial antimicrobiano em algumas das variáveis testadas. Portanto, devem ser realizados estudos adicionais para comprovar a bioatividade antifúngica in vivo das espécies C. peltophoroides e P. heptaphyllum e, identificar os compostos bioativos específicos e seu modo de ação, para que possam futuramente ser utilizado como alternativa aos fungicidas.Universidade Federal de Mato GrossoBrasilInstituto de Ciências Agrárias e Ambientais (ICAA) – SinopUFMT CUS - SinopPrograma de Pós-Graduação em Ciências AmbientaisBonaldo, Solange MariaVieira Júnior, Gerardo Magelahttp://lattes.cnpq.br/8484684765493808http://lattes.cnpq.br/3703629752105309Bonaldo, Solange Maria015.899.719-09http://lattes.cnpq.br/3703629752105309Andrighetti, Carla Regina851.533.549-20http://lattes.cnpq.br/8724014228425230015.899.719-09649.899.763-04Kummer, Raquel Kummer056.541.089-05http://lattes.cnpq.br/5423995543057678Lima, Airton2020-10-07T13:38:31Z2017-03-092020-10-07T13:38:31Z2016-03-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLIMA, Airton. Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos. 2016. 38 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências Naturais, Humanas e Sociais, Sinop, 2016.http://ri.ufmt.br/handle/1/2117porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2020-10-15T07:01:20Zoai:localhost:1/2117Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2020-10-15T07:01:20Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
title |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
spellingShingle |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos Lima, Airton Agrotóxicos Medidas alternativas Compostos bioativos Atividade antifúngica CNPQ::OUTROS::CIENCIAS Agrochemicals Alternative measures Bioactive compounds Antifungal activity |
title_short |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
title_full |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
title_fullStr |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
title_full_unstemmed |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
title_sort |
Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos |
author |
Lima, Airton |
author_facet |
Lima, Airton |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bonaldo, Solange Maria Vieira Júnior, Gerardo Magela http://lattes.cnpq.br/8484684765493808 http://lattes.cnpq.br/3703629752105309 Bonaldo, Solange Maria 015.899.719-09 http://lattes.cnpq.br/3703629752105309 Andrighetti, Carla Regina 851.533.549-20 http://lattes.cnpq.br/8724014228425230 015.899.719-09 649.899.763-04 Kummer, Raquel Kummer 056.541.089-05 http://lattes.cnpq.br/5423995543057678 |
dc.contributor.author.fl_str_mv |
Lima, Airton |
dc.subject.por.fl_str_mv |
Agrotóxicos Medidas alternativas Compostos bioativos Atividade antifúngica CNPQ::OUTROS::CIENCIAS Agrochemicals Alternative measures Bioactive compounds Antifungal activity |
topic |
Agrotóxicos Medidas alternativas Compostos bioativos Atividade antifúngica CNPQ::OUTROS::CIENCIAS Agrochemicals Alternative measures Bioactive compounds Antifungal activity |
description |
The Brazilian biodiversity has been studied by several researches that are looking for determine the effect of plant extracts against plant pathogens, as an alternative to the use of chemicals products. Face to this, the present research aimed to study part of the brasilian flora in order to verify the effect of extracts of sibipiruna leaves (Caesalpinia peltophoroides) in three Colletotrichum sp using two methodologies: evaluate the fungitoxic effect of different extracts concentrations of C. peltophoroides and white pitch (Protium heptaphyllum) against Colletotrichum truncatum, Colletotrichum sp., Fusarium equiseti, F. prolifetarum and F. solani. First, tests were performed with plant pathogens Colletotrichum sp. and C. truncatum isolated of soy beans from three different locations, through the methods of disk diffusion and spreading. It was used the ethereal fraction of leaves of C. peltophoroides and ethanolic extract of leaves of P. heptaphyllum against isolates of C. truncatum, Colletotrichum sp., F. equiseti, F. solani and F. prolifetarum tested in various concentrations for disk diffusion method. It was found the percentages in inhibition of mycelial growth, mycelial growth rate index, inhibition of sporulation, germination, and training appressoria. The ethanolic extract from the leaves of C. peltophoroides shows antifungal activity against three kinds of Colletotrichum, it was observed that the reduced speed of mycelial growth, which was similar to the fungicide. Inhibit sporulation, in some concentrations, above 60%; and the appressorium formation to 92.30%, proving to be effective. The methodology scattering when compared to disk diffusion showed better results for the evaluation of antifungal activity in the inhibition of sporulation. The C. peltophoroides extract was effective in all tested variables, with satisfactory results in at least one of the isolated and evaluated species. The C. peltophoroides extract has antifungal activity and can be an efficient and environmentally friendly alternative for the control of plant pathogens. The P. heptaphyllum extract did not inhibit mycelial growth and sporulation of the evaluated plant pathogens; however, inhibit the germination and the formation of appressoria, especially species of Fusarium. From the results obtained in this study, one can conclude that the ethanolic extracts of leaves of C. peltophoroides and P. heptaphyllum have antimicrobial potential on some of the variables tested. Therefore, further studies should be performed to confirm the in vivo bioactivity of antifungal in vivo species of C. peltophoroides and P. heptaphyllum and identify specific bioactive compounds and their mode of action, so that they can eventually be used as an alternative to fungicides. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-29 2017-03-09 2020-10-07T13:38:31Z 2020-10-07T13:38:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LIMA, Airton. Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos. 2016. 38 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências Naturais, Humanas e Sociais, Sinop, 2016. http://ri.ufmt.br/handle/1/2117 |
identifier_str_mv |
LIMA, Airton. Potencial antimicrobiano de extratos etanólicos de folhas de sibipiruna (Caesalpinia peltophoroides) e breu branco (Protium heptaphyllum) em fitopatógenos. 2016. 38 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências Naturais, Humanas e Sociais, Sinop, 2016. |
url |
http://ri.ufmt.br/handle/1/2117 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Agrárias e Ambientais (ICAA) – Sinop UFMT CUS - Sinop Programa de Pós-Graduação em Ciências Ambientais |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Instituto de Ciências Agrárias e Ambientais (ICAA) – Sinop UFMT CUS - Sinop Programa de Pós-Graduação em Ciências Ambientais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
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Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
institution |
UFMT |
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Repositório Institucional da UFMT |
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Repositório Institucional da UFMT |
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Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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jordanbiblio@gmail.com |
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1804648503732862976 |