DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1007 |
Resumo: | With the increase in life expectancy and growth in the number of elderly people in the world, it is estimated that there is a high prevalence of neurodegenerative diseases, especially Alzheimer's disease. Treatment for this population can be through the use of drugs or non-pharmacological therapy. The effects of drugs are limited to delaying or halting the evolution of the disease, allowing only a temporary improvement in the patient. In relation to non-pharmacological therapy, aromatherapy stands out, which is part of phytotherapy and uses essential oils as therapeutics, but its disadvantage is high volatilization. As a way to protect its active compounds and ensure the effectiveness of these oils, the association of memos with nanotechnology stands out, which due to its size and characteristics can increase the mechanisms of cellular absorption, as well as its effectiveness. Thus, the aim of this study was to evaluate the feasibility of associating essential oils from two species of lavender in nanotechnology-based formulations and verify their safety profile, as well as their efficacy in an in vivo model of neuroprotection. This is an experimental study, where nanoemulsions containing L. angustifolia essential oil (NELA) and nanoemulsions containing L. dentata essential oil (NELD) were prepared and characterized, followed by their incorporation in hydrogels, as well as physical characterization -chemistry and stability evaluation. Afterwards, the safety and efficacy of the formulations was evaluated using the C. elegans model (CL2006). The results showed that liquid and semi-solid nanoformulations demonstrated adequate nanometric properties. The major components present in the essential oils, of each lavender species, were preserved in the nanoformulations, during the stability study at the refrigeration temperature. In the in vivo analysis we concluded that the doses of 0.1 and 2.5% of the nanoformulations did not change the survival rate of C. elegans after 1 hour of treatment, in addition, the treatment with 0.1% of both essential oils free did not alter the survival rate of C. elegans. In contrast, doses of 2.5 and 10% of both free L. angustifolia and L. dentata essential oils decreased the survival rate of C. elegans compared to the control group. When comparing the effectiveness of 0.1% and 2.5% nanoformulations, we observed that the responses for all formulations were better at the dose of 0.1% and that the nanoformulation that showed better responses in relation to paralysis was the one with GNELD, that is, with the essential oil of L. dentata. Thus, we can conclude that nanoformulations containing L. angustifolia and L. dentata essential oil at a concentration of 0.1% were safe and effective in a C. elegans model (CL2006). |
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Ourique, Aline FerreiraBoeck, Carina RodriguesHidalgo, Maria Pilar Vinardell MartínezSilva, Cristiane de Bona daPeroza, Luis RicardoSilva, William Leonardo daNedel, Sheila Spohr2021-09-21T20:02:52Z2022-08-242021-07-14Nedel, Sheila Spohr. DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO. 2021. 81f. Tese( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1007With the increase in life expectancy and growth in the number of elderly people in the world, it is estimated that there is a high prevalence of neurodegenerative diseases, especially Alzheimer's disease. Treatment for this population can be through the use of drugs or non-pharmacological therapy. The effects of drugs are limited to delaying or halting the evolution of the disease, allowing only a temporary improvement in the patient. In relation to non-pharmacological therapy, aromatherapy stands out, which is part of phytotherapy and uses essential oils as therapeutics, but its disadvantage is high volatilization. As a way to protect its active compounds and ensure the effectiveness of these oils, the association of memos with nanotechnology stands out, which due to its size and characteristics can increase the mechanisms of cellular absorption, as well as its effectiveness. Thus, the aim of this study was to evaluate the feasibility of associating essential oils from two species of lavender in nanotechnology-based formulations and verify their safety profile, as well as their efficacy in an in vivo model of neuroprotection. This is an experimental study, where nanoemulsions containing L. angustifolia essential oil (NELA) and nanoemulsions containing L. dentata essential oil (NELD) were prepared and characterized, followed by their incorporation in hydrogels, as well as physical characterization -chemistry and stability evaluation. Afterwards, the safety and efficacy of the formulations was evaluated using the C. elegans model (CL2006). The results showed that liquid and semi-solid nanoformulations demonstrated adequate nanometric properties. The major components present in the essential oils, of each lavender species, were preserved in the nanoformulations, during the stability study at the refrigeration temperature. In the in vivo analysis we concluded that the doses of 0.1 and 2.5% of the nanoformulations did not change the survival rate of C. elegans after 1 hour of treatment, in addition, the treatment with 0.1% of both essential oils free did not alter the survival rate of C. elegans. In contrast, doses of 2.5 and 10% of both free L. angustifolia and L. dentata essential oils decreased the survival rate of C. elegans compared to the control group. When comparing the effectiveness of 0.1% and 2.5% nanoformulations, we observed that the responses for all formulations were better at the dose of 0.1% and that the nanoformulation that showed better responses in relation to paralysis was the one with GNELD, that is, with the essential oil of L. dentata. Thus, we can conclude that nanoformulations containing L. angustifolia and L. dentata essential oil at a concentration of 0.1% were safe and effective in a C. elegans model (CL2006).Com o aumento da expectativa de vida e crescimento do número de idosos no mundo, estima-se que se tenha em consequência, uma alta prevalência das doenças neurodegenerativas, com destaque para a doença de Alzheimer. O tratamento para essa população, pode ser através do uso de fármacos ou de terapia não farmacológica. Os efeitos dos fármacos limitam-se ao retardo ou estacionam a evolução da doença, permitindo apenas uma melhora temporária do paciente. Em relação a terapia não farmacológica, destaca- se a aromaterapia, a qual faz parte da fitoterapia e utiliza óleos essenciais como terapêutica, porém apresentam como desvantagem, a alta volatilização. Como forma de proteger seus compostos ativos e garantir a eficácia desses óleos, destaca-se a associação dos mesmos com a nanotecnologia, a qual devido a seu tamanho e características pode aumentar os mecanismos de absorção celular, assim como, eficácia. Dessa forma, o objetivo desse estudo foi avaliar a viabilidade de associar óleos essenciais de duas espécies de lavanda em formulações de base nanotecnológica e verificar seu perfil de segurança, bem como, sua eficácia em modelo in vivo de neuroproteção. Trata-se de um estudo experimental, onde foram preparadas e caracterizadas nanoemulsões contendo óleo essencial de L. angustifolia (NELA) e nanoemulsões contendo óleo essencial de L. dentata (NELD) seguido da sua incorporação em hidrogéis, bem como, caracterização físico-química e avaliação de estabilidade. Após, foi avaliada a segurança e eficácia das formulações através de modelo de C. elegans (CL2006). Os resultados evidenciaram que as nanoformulações líquidas e semissólidas demonstraram propriedades nanométricas adequadas. Os componentes majoritários presentes nos óleos essenciais, de cada espécie de lavanda, mantiveram-se preservados nas nanoformulações, durante o estudo de estabilidade na temperatura de refrigeração. Na análise in vivo concluímos que as doses de 0,1 e 2,5% das nanoformulações não alteraram a taxa de sobrevivência de C. elegans após 1 hora de tratamento, além disso, o tratamento com 0,1 % de ambos os óleos essenciais livres não alterou a taxa de sobrevivência de C. elegans. Em contrapartida, as doses de 2,5 e 10% de ambos os óleos essenciais de L. angustifolia e L. dentata livres diminuíram a taxa de sobrevivência de C. elegans em comparação com o grupo de controle. Ao compararmos a eficácia das nanoformulações de 0,1% e 2,5% observamos que as respostas para todas as formulações foram melhores na dose de 0,1% e que a nanoformulação que apresentou melhores respostas em relação a paralisia foi a com GNELD, ou seja, a com o óleo essencial de L. dentata. Assim, podemos concluir que as nanoformulações contendo óleo essencial de L. angustifolia e L. dentata na concentração de 0,1 % foram seguras e ficazes em modelo de C. elegans (CL2006).Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2021-09-21T20:02:52Z No. of bitstreams: 3 Tese_SheilaSpohrNedel_VersaoParcial.pdf: 2030398 bytes, checksum: a4f46fe35dd56ce2555b2b0ce8f2724d (MD5) Tese_SheilaSpohrNedel.pdf: 3656070 bytes, checksum: 39ceac9409b01667d1a4e6cb047cbb61 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2021-09-21T20:02:52Z (GMT). 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dc.title.por.fl_str_mv |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
title |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
spellingShingle |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO Nedel, Sheila Spohr Lavandula angustifolia; Lavandula dentata, nanoemulsão, nanogél, hidrogél, doenças neurodegenerativas. Lavandula angustifolia; Lavandula dentata, nanoemulsion, nanogel, hydrogel, neurodegenerative diseases. Nanociências |
title_short |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
title_full |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
title_fullStr |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
title_full_unstemmed |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
title_sort |
DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO |
author |
Nedel, Sheila Spohr |
author_facet |
Nedel, Sheila Spohr |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ourique, Aline Ferreira |
dc.contributor.advisor-co1.fl_str_mv |
Boeck, Carina Rodrigues |
dc.contributor.referee1.fl_str_mv |
Hidalgo, Maria Pilar Vinardell Martínez |
dc.contributor.referee2.fl_str_mv |
Silva, Cristiane de Bona da |
dc.contributor.referee3.fl_str_mv |
Peroza, Luis Ricardo |
dc.contributor.referee4.fl_str_mv |
Silva, William Leonardo da |
dc.contributor.author.fl_str_mv |
Nedel, Sheila Spohr |
contributor_str_mv |
Ourique, Aline Ferreira Boeck, Carina Rodrigues Hidalgo, Maria Pilar Vinardell Martínez Silva, Cristiane de Bona da Peroza, Luis Ricardo Silva, William Leonardo da |
dc.subject.por.fl_str_mv |
Lavandula angustifolia; Lavandula dentata, nanoemulsão, nanogél, hidrogél, doenças neurodegenerativas. |
topic |
Lavandula angustifolia; Lavandula dentata, nanoemulsão, nanogél, hidrogél, doenças neurodegenerativas. Lavandula angustifolia; Lavandula dentata, nanoemulsion, nanogel, hydrogel, neurodegenerative diseases. Nanociências |
dc.subject.eng.fl_str_mv |
Lavandula angustifolia; Lavandula dentata, nanoemulsion, nanogel, hydrogel, neurodegenerative diseases. |
dc.subject.cnpq.fl_str_mv |
Nanociências |
description |
With the increase in life expectancy and growth in the number of elderly people in the world, it is estimated that there is a high prevalence of neurodegenerative diseases, especially Alzheimer's disease. Treatment for this population can be through the use of drugs or non-pharmacological therapy. The effects of drugs are limited to delaying or halting the evolution of the disease, allowing only a temporary improvement in the patient. In relation to non-pharmacological therapy, aromatherapy stands out, which is part of phytotherapy and uses essential oils as therapeutics, but its disadvantage is high volatilization. As a way to protect its active compounds and ensure the effectiveness of these oils, the association of memos with nanotechnology stands out, which due to its size and characteristics can increase the mechanisms of cellular absorption, as well as its effectiveness. Thus, the aim of this study was to evaluate the feasibility of associating essential oils from two species of lavender in nanotechnology-based formulations and verify their safety profile, as well as their efficacy in an in vivo model of neuroprotection. This is an experimental study, where nanoemulsions containing L. angustifolia essential oil (NELA) and nanoemulsions containing L. dentata essential oil (NELD) were prepared and characterized, followed by their incorporation in hydrogels, as well as physical characterization -chemistry and stability evaluation. Afterwards, the safety and efficacy of the formulations was evaluated using the C. elegans model (CL2006). The results showed that liquid and semi-solid nanoformulations demonstrated adequate nanometric properties. The major components present in the essential oils, of each lavender species, were preserved in the nanoformulations, during the stability study at the refrigeration temperature. In the in vivo analysis we concluded that the doses of 0.1 and 2.5% of the nanoformulations did not change the survival rate of C. elegans after 1 hour of treatment, in addition, the treatment with 0.1% of both essential oils free did not alter the survival rate of C. elegans. In contrast, doses of 2.5 and 10% of both free L. angustifolia and L. dentata essential oils decreased the survival rate of C. elegans compared to the control group. When comparing the effectiveness of 0.1% and 2.5% nanoformulations, we observed that the responses for all formulations were better at the dose of 0.1% and that the nanoformulation that showed better responses in relation to paralysis was the one with GNELD, that is, with the essential oil of L. dentata. Thus, we can conclude that nanoformulations containing L. angustifolia and L. dentata essential oil at a concentration of 0.1% were safe and effective in a C. elegans model (CL2006). |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-09-21T20:02:52Z |
dc.date.issued.fl_str_mv |
2021-07-14 |
dc.date.available.fl_str_mv |
2022-08-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Nedel, Sheila Spohr. DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO. 2021. 81f. Tese( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS . |
dc.identifier.uri.fl_str_mv |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1007 |
identifier_str_mv |
Nedel, Sheila Spohr. DESENVOLVIMENTO, AVALIAÇÃO DO PERFIL DE SEGURANÇA E EFICÁCIA DE SISTEMAS NANOESTRUTURADOS CONTENDO ÓLEO ESSENCIAL DE LAVANDA NA NEUROPROTEÇÃO. 2021. 81f. Tese( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS . |
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http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1007 |
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por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidade Franciscana |
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Programa de Pós-Graduação em Nanociências |
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UFN |
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Brasil |
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Biociências e Nanomateriais |
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Universidade Franciscana |
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reponame:Repositório Institucional Universidade Franciscana instname:Universidade Franciscana (UFN) instacron:UFN |
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Universidade Franciscana (UFN) |
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UFN |
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Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN) |
repository.mail.fl_str_mv |
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1801046210806546432 |