Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/728 |
Resumo: | Viticulture in Brazil has a great economic importance and an extension of approximately 80,000 hectares, generating employment and income for several families, however it is quite prone to infectious processes, which drastically reduces its productivity. Among these agents is Glomerella cingulata, which is controlled by fungicides, such as mancozeb. However, the use of this commercial product is related to contamination of the environment, in addition to a known toxic effect for humans. In this context the use of a naturally occurring substance, such as eugenol, could be a promising alternative. However, eugenol, which has an antimicrobial action, presents high volatilization and low solubility in water, thus impairing its administration and efficacy. One possibility to minimize toxicity of the fungicide, and the instability of eugenol, is nanoencapsulation. Therefore, the aim of this work was to verify the feasibility of co-encapsulation of mancozeb and eugenol in nanoemulsions and to evaluate the potential antifungal activity of nanoemulsions containing these compounds isolated and associated with Glomerella cingulata. Nanoemulsions containing eugenol (NE-E), mancozeb and eugenol (NE-ME), nanoemulsions with mancozeb (NE-M) and white nanoemulsions, without the active ones (NE-B) were prepared by the spontaneous emulsification method. The formulations were characterized according to mean diameter, polydispersity index (PDI), zeta potential, pH, content and morphology. The stability of the formulations for 30 days under different storage conditions and the antifungal activity against G. cingulata were also evaluated. The nanoemulsions had a mean diameter of approximately 200 nm, PDI of approximately 0.170, zeta potential of -11.0 mV, pH close to 5.0 and eugenol content, for NE-E and NE-ME, around 40%. Transmission electron microscopy showed spherical shape. The antifungal activity assay was performed by the macrodilution technique, and the active compounds were analyzed: free eugenol, mancozeb, mancozeb and eugenol and the same nanoencapsulated ones. The NE-M obtained a minimum inhibitory activity of 0.009 μg / mL, a satisfactory result, followed by NE-ME 0.02 μg / mL mancozeb + 2 μg/mL eugenol compared to its free forms. The best condition for storage of the formulations was under refrigeration and antifungal activity was potentiated after nanoencapsulation for all formulations. This study presents an innovative alternative for the treatment of G. cingulata through the use of nanotechnology. |
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Ourique, Aline FerreiraSantos, Roberto Christ ViannCodevilla, Cristiane FrancoSantos, Claudia Lange dosNascimento, Daniela Alves de Oliveira2019-04-23T13:44:12Z2017-09-26Nascimento, Daniela Alves de Oliveira. Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol. 2017. 52f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/728Viticulture in Brazil has a great economic importance and an extension of approximately 80,000 hectares, generating employment and income for several families, however it is quite prone to infectious processes, which drastically reduces its productivity. Among these agents is Glomerella cingulata, which is controlled by fungicides, such as mancozeb. However, the use of this commercial product is related to contamination of the environment, in addition to a known toxic effect for humans. In this context the use of a naturally occurring substance, such as eugenol, could be a promising alternative. However, eugenol, which has an antimicrobial action, presents high volatilization and low solubility in water, thus impairing its administration and efficacy. One possibility to minimize toxicity of the fungicide, and the instability of eugenol, is nanoencapsulation. Therefore, the aim of this work was to verify the feasibility of co-encapsulation of mancozeb and eugenol in nanoemulsions and to evaluate the potential antifungal activity of nanoemulsions containing these compounds isolated and associated with Glomerella cingulata. Nanoemulsions containing eugenol (NE-E), mancozeb and eugenol (NE-ME), nanoemulsions with mancozeb (NE-M) and white nanoemulsions, without the active ones (NE-B) were prepared by the spontaneous emulsification method. The formulations were characterized according to mean diameter, polydispersity index (PDI), zeta potential, pH, content and morphology. The stability of the formulations for 30 days under different storage conditions and the antifungal activity against G. cingulata were also evaluated. The nanoemulsions had a mean diameter of approximately 200 nm, PDI of approximately 0.170, zeta potential of -11.0 mV, pH close to 5.0 and eugenol content, for NE-E and NE-ME, around 40%. Transmission electron microscopy showed spherical shape. The antifungal activity assay was performed by the macrodilution technique, and the active compounds were analyzed: free eugenol, mancozeb, mancozeb and eugenol and the same nanoencapsulated ones. The NE-M obtained a minimum inhibitory activity of 0.009 μg / mL, a satisfactory result, followed by NE-ME 0.02 μg / mL mancozeb + 2 μg/mL eugenol compared to its free forms. The best condition for storage of the formulations was under refrigeration and antifungal activity was potentiated after nanoencapsulation for all formulations. This study presents an innovative alternative for the treatment of G. cingulata through the use of nanotechnology.A viticultura no Brasil apresenta uma grande importância econômica e uma extensão de aproximadamente 80.000 hectares, gerando emprego e renda para várias famílias, entretanto é bastante propensa a processos infecciosos, o que diminui drasticamente sua produtividade. Dentre estes agentes está a Glomerella cingulata, cujo controle é realizado por fungicidas, como o mancozebe. Entretanto o uso deste produto comercial está relacionado com contaminação do ambiente, além de conhecido efeito tóxico para os seres humanos. Neste contexto o uso de substância de origem natural, como o eugenol, poderia ser uma alternativa promissora. Entretanto, o eugenol, que possui ação antimicrobiana, apresenta alta volatilização e baixa solubilidade em água, prejudicando assim sua administração e eficácia. Uma possibilidade para minimizar a toxicidade do fungicida, e a instabilidade do eugenol, é a nanoencapsulação. Sendo assim, a proposta deste trabalho foi verificar a viabilidade de co-encapsulação do mancozebe e do eugenol em nanoemulsões e avaliar a potencial atividade antifúngica de nanoemulsões contendo estes compostos isolados e associados frente à Glomerella cingulata. Foram preparadas nanoemulsões contendo eugenol (NE-E), mancozebe e eugenol (NE- ME), nanoemulsões com mancozebe (NE-M) e nanoemulsões brancas, sem os ativos (NE-B) através do método emulsificação espontânea. As formulações, foram caracterizadas de acordo com diâmetro médio, índice de polidispersão (PDI), potencial zeta, pH, teor e morfologia. Também foi avaliada a estabilidade das formulações durante 30 dias em diferentes condições de armazenamento e a atividade antifúngica frente à G. cingulata. As nanoemulsões apresentaram diâmetro médio de aproximadamente 200 nm, PDI de aproximadamente 0,170, potencial zeta de -11,0 mV, pH próximo à 5,0 e teor de eugenol, para NE-E e NE-ME, em torno de 40%. A microscopia eletrônica de transmissão demonstrou formato esférico. O ensaio da atividade antifúngica foi realizado pela técnica de macrodiluição, sendo analisados os ativos: eugenol, mancozebe, mancozebe e eugenol livres e os mesmos nanoencapsulados. A NE-M, obteve uma atividade inibitória mínima de 0,009 μg/mL, resultado satisfatório, seguida da NE-ME 0,02 μg/mL de mancozebe e 2 μg/mL de eugenol, comparada a suas formas livres. A melhor condição para armazenamento das formulações foi sob refrigeração e a atividade antifúngica foi potencializada após nanoencapsulação para todas as formulações. Este estudo apresenta uma alternativa inovadora para o tratamento da G. cingulata através do emprego da nanotecnologia.Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2019-04-23T13:44:12Z No. of bitstreams: 2 Dissertacao_DanielaAlvesDeOliveira.pdf: 1704710 bytes, checksum: 2a7d863c9c37b44daa7f6af4648c5cd2 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-04-23T13:44:12Z (GMT). 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dc.title.por.fl_str_mv |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
title |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
spellingShingle |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol Nascimento, Daniela Alves de Oliveira emulsificação espontânea, fungicida, óleo, CLAE, nanotecnologia Spontaneous emulsification, fungicide, oil, HPLC, nanotechnology. Nanociências |
title_short |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
title_full |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
title_fullStr |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
title_full_unstemmed |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
title_sort |
Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol |
author |
Nascimento, Daniela Alves de Oliveira |
author_facet |
Nascimento, Daniela Alves de Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ourique, Aline Ferreira |
dc.contributor.advisor-co1.fl_str_mv |
Santos, Roberto Christ Viann |
dc.contributor.referee1.fl_str_mv |
Codevilla, Cristiane Franco |
dc.contributor.referee2.fl_str_mv |
Santos, Claudia Lange dos |
dc.contributor.author.fl_str_mv |
Nascimento, Daniela Alves de Oliveira |
contributor_str_mv |
Ourique, Aline Ferreira Santos, Roberto Christ Viann Codevilla, Cristiane Franco Santos, Claudia Lange dos |
dc.subject.por.fl_str_mv |
emulsificação espontânea, fungicida, óleo, CLAE, nanotecnologia |
topic |
emulsificação espontânea, fungicida, óleo, CLAE, nanotecnologia Spontaneous emulsification, fungicide, oil, HPLC, nanotechnology. Nanociências |
dc.subject.eng.fl_str_mv |
Spontaneous emulsification, fungicide, oil, HPLC, nanotechnology. |
dc.subject.cnpq.fl_str_mv |
Nanociências |
description |
Viticulture in Brazil has a great economic importance and an extension of approximately 80,000 hectares, generating employment and income for several families, however it is quite prone to infectious processes, which drastically reduces its productivity. Among these agents is Glomerella cingulata, which is controlled by fungicides, such as mancozeb. However, the use of this commercial product is related to contamination of the environment, in addition to a known toxic effect for humans. In this context the use of a naturally occurring substance, such as eugenol, could be a promising alternative. However, eugenol, which has an antimicrobial action, presents high volatilization and low solubility in water, thus impairing its administration and efficacy. One possibility to minimize toxicity of the fungicide, and the instability of eugenol, is nanoencapsulation. Therefore, the aim of this work was to verify the feasibility of co-encapsulation of mancozeb and eugenol in nanoemulsions and to evaluate the potential antifungal activity of nanoemulsions containing these compounds isolated and associated with Glomerella cingulata. Nanoemulsions containing eugenol (NE-E), mancozeb and eugenol (NE-ME), nanoemulsions with mancozeb (NE-M) and white nanoemulsions, without the active ones (NE-B) were prepared by the spontaneous emulsification method. The formulations were characterized according to mean diameter, polydispersity index (PDI), zeta potential, pH, content and morphology. The stability of the formulations for 30 days under different storage conditions and the antifungal activity against G. cingulata were also evaluated. The nanoemulsions had a mean diameter of approximately 200 nm, PDI of approximately 0.170, zeta potential of -11.0 mV, pH close to 5.0 and eugenol content, for NE-E and NE-ME, around 40%. Transmission electron microscopy showed spherical shape. The antifungal activity assay was performed by the macrodilution technique, and the active compounds were analyzed: free eugenol, mancozeb, mancozeb and eugenol and the same nanoencapsulated ones. The NE-M obtained a minimum inhibitory activity of 0.009 μg / mL, a satisfactory result, followed by NE-ME 0.02 μg / mL mancozeb + 2 μg/mL eugenol compared to its free forms. The best condition for storage of the formulations was under refrigeration and antifungal activity was potentiated after nanoencapsulation for all formulations. This study presents an innovative alternative for the treatment of G. cingulata through the use of nanotechnology. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-09-26 |
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2019-04-23T13:44:12Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
Nascimento, Daniela Alves de Oliveira. Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol. 2017. 52f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
dc.identifier.uri.fl_str_mv |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/728 |
identifier_str_mv |
Nascimento, Daniela Alves de Oliveira. Desenvolvimento, caracterização e atividade antifúngica frente à Glomerella cingulata de nanoemulsões contendo mancozebe e eugenol. 2017. 52f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
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UFN |
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Brasil |
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Biociências e Nanomateriais |
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Universidade Franciscana |
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