Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.

Detalhes bibliográficos
Autor(a) principal: Silva, Eduardo de Almeida Marques da
Data de Publicação: 2008
Outros Autores: Oliveira, Jamile Camargos de, Figueiredo, Amanda Braga de, Lima Júnior, Djalma de Souza, Carneiro, Cláudia Martins, Fietto, Juliana Lopes Rangel, Afonso, Luís Carlos Crocco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/1404
Resumo: Leishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of extracellular adenosine, in the establishment of Leishmania infection. Our results show that the more virulent parasitedL. Amazonensisdhydrolyzes higher amounts of ATP, ADP and AMP than the two other species, probably due to the higher expression of membrane NTPDase. Corroborating the idea that increased production of adenosine is important to lesion development and establishment of tissue parasitism, we observed that increased 50-nucleotidase activity in L. braziliensis or addition of adenosine at the moment of infection with this parasite resulted in an increase in lesion size and parasitism as well as a delay in lesion healing. Furthermore, inhibition of adenosine receptor A2B led to decreased lesion size and parasitism. Thus, our results suggest that the conversion of ATP, a molecule with pro-inflammatory activity, into adenosine, which possesses immunomodulatory properties, may contribute to the establishment of infection by Leishmania.
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spelling Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.Ecto-nucleotidaseAdenosineLeishmaniaLeishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of extracellular adenosine, in the establishment of Leishmania infection. Our results show that the more virulent parasitedL. Amazonensisdhydrolyzes higher amounts of ATP, ADP and AMP than the two other species, probably due to the higher expression of membrane NTPDase. Corroborating the idea that increased production of adenosine is important to lesion development and establishment of tissue parasitism, we observed that increased 50-nucleotidase activity in L. braziliensis or addition of adenosine at the moment of infection with this parasite resulted in an increase in lesion size and parasitism as well as a delay in lesion healing. Furthermore, inhibition of adenosine receptor A2B led to decreased lesion size and parasitism. Thus, our results suggest that the conversion of ATP, a molecule with pro-inflammatory activity, into adenosine, which possesses immunomodulatory properties, may contribute to the establishment of infection by Leishmania.2012-09-19T15:38:09Z2012-09-19T15:38:09Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, E. de A. M. da et al. Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Microbes and Infection, v. 10, n. 8, p. 850-857, jul. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1286457908001202>. Acesso em: 19 ago. 2012.12864579http://www.repositorio.ufop.br/handle/123456789/1404O periódico Microbes and Infection concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281990186750.info:eu-repo/semantics/openAccessSilva, Eduardo de Almeida Marques daOliveira, Jamile Camargos deFigueiredo, Amanda Braga deLima Júnior, Djalma de SouzaCarneiro, Cláudia MartinsFietto, Juliana Lopes RangelAfonso, Luís Carlos Croccoengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-03-08T16:33:02Zoai:repositorio.ufop.br:123456789/1404Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-08T16:33:02Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
title Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
spellingShingle Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
Silva, Eduardo de Almeida Marques da
Ecto-nucleotidase
Adenosine
Leishmania
title_short Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
title_full Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
title_fullStr Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
title_full_unstemmed Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
title_sort Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
author Silva, Eduardo de Almeida Marques da
author_facet Silva, Eduardo de Almeida Marques da
Oliveira, Jamile Camargos de
Figueiredo, Amanda Braga de
Lima Júnior, Djalma de Souza
Carneiro, Cláudia Martins
Fietto, Juliana Lopes Rangel
Afonso, Luís Carlos Crocco
author_role author
author2 Oliveira, Jamile Camargos de
Figueiredo, Amanda Braga de
Lima Júnior, Djalma de Souza
Carneiro, Cláudia Martins
Fietto, Juliana Lopes Rangel
Afonso, Luís Carlos Crocco
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Eduardo de Almeida Marques da
Oliveira, Jamile Camargos de
Figueiredo, Amanda Braga de
Lima Júnior, Djalma de Souza
Carneiro, Cláudia Martins
Fietto, Juliana Lopes Rangel
Afonso, Luís Carlos Crocco
dc.subject.por.fl_str_mv Ecto-nucleotidase
Adenosine
Leishmania
topic Ecto-nucleotidase
Adenosine
Leishmania
description Leishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of extracellular adenosine, in the establishment of Leishmania infection. Our results show that the more virulent parasitedL. Amazonensisdhydrolyzes higher amounts of ATP, ADP and AMP than the two other species, probably due to the higher expression of membrane NTPDase. Corroborating the idea that increased production of adenosine is important to lesion development and establishment of tissue parasitism, we observed that increased 50-nucleotidase activity in L. braziliensis or addition of adenosine at the moment of infection with this parasite resulted in an increase in lesion size and parasitism as well as a delay in lesion healing. Furthermore, inhibition of adenosine receptor A2B led to decreased lesion size and parasitism. Thus, our results suggest that the conversion of ATP, a molecule with pro-inflammatory activity, into adenosine, which possesses immunomodulatory properties, may contribute to the establishment of infection by Leishmania.
publishDate 2008
dc.date.none.fl_str_mv 2008
2012-09-19T15:38:09Z
2012-09-19T15:38:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, E. de A. M. da et al. Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Microbes and Infection, v. 10, n. 8, p. 850-857, jul. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1286457908001202>. Acesso em: 19 ago. 2012.
12864579
http://www.repositorio.ufop.br/handle/123456789/1404
identifier_str_mv SILVA, E. de A. M. da et al. Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Microbes and Infection, v. 10, n. 8, p. 850-857, jul. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1286457908001202>. Acesso em: 19 ago. 2012.
12864579
url http://www.repositorio.ufop.br/handle/123456789/1404
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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