Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/1404 |
Resumo: | Leishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of extracellular adenosine, in the establishment of Leishmania infection. Our results show that the more virulent parasitedL. Amazonensisdhydrolyzes higher amounts of ATP, ADP and AMP than the two other species, probably due to the higher expression of membrane NTPDase. Corroborating the idea that increased production of adenosine is important to lesion development and establishment of tissue parasitism, we observed that increased 50-nucleotidase activity in L. braziliensis or addition of adenosine at the moment of infection with this parasite resulted in an increase in lesion size and parasitism as well as a delay in lesion healing. Furthermore, inhibition of adenosine receptor A2B led to decreased lesion size and parasitism. Thus, our results suggest that the conversion of ATP, a molecule with pro-inflammatory activity, into adenosine, which possesses immunomodulatory properties, may contribute to the establishment of infection by Leishmania. |
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Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection.Ecto-nucleotidaseAdenosineLeishmaniaLeishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of extracellular adenosine, in the establishment of Leishmania infection. Our results show that the more virulent parasitedL. Amazonensisdhydrolyzes higher amounts of ATP, ADP and AMP than the two other species, probably due to the higher expression of membrane NTPDase. Corroborating the idea that increased production of adenosine is important to lesion development and establishment of tissue parasitism, we observed that increased 50-nucleotidase activity in L. braziliensis or addition of adenosine at the moment of infection with this parasite resulted in an increase in lesion size and parasitism as well as a delay in lesion healing. Furthermore, inhibition of adenosine receptor A2B led to decreased lesion size and parasitism. Thus, our results suggest that the conversion of ATP, a molecule with pro-inflammatory activity, into adenosine, which possesses immunomodulatory properties, may contribute to the establishment of infection by Leishmania.2012-09-19T15:38:09Z2012-09-19T15:38:09Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, E. de A. M. da et al. Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Microbes and Infection, v. 10, n. 8, p. 850-857, jul. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1286457908001202>. Acesso em: 19 ago. 2012.12864579http://www.repositorio.ufop.br/handle/123456789/1404O periódico Microbes and Infection concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281990186750.info:eu-repo/semantics/openAccessSilva, Eduardo de Almeida Marques daOliveira, Jamile Camargos deFigueiredo, Amanda Braga deLima Júnior, Djalma de SouzaCarneiro, Cláudia MartinsFietto, Juliana Lopes RangelAfonso, Luís Carlos Croccoengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-03-08T16:33:02Zoai:repositorio.ufop.br:123456789/1404Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-08T16:33:02Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
title |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
spellingShingle |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Silva, Eduardo de Almeida Marques da Ecto-nucleotidase Adenosine Leishmania |
title_short |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
title_full |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
title_fullStr |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
title_full_unstemmed |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
title_sort |
Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. |
author |
Silva, Eduardo de Almeida Marques da |
author_facet |
Silva, Eduardo de Almeida Marques da Oliveira, Jamile Camargos de Figueiredo, Amanda Braga de Lima Júnior, Djalma de Souza Carneiro, Cláudia Martins Fietto, Juliana Lopes Rangel Afonso, Luís Carlos Crocco |
author_role |
author |
author2 |
Oliveira, Jamile Camargos de Figueiredo, Amanda Braga de Lima Júnior, Djalma de Souza Carneiro, Cláudia Martins Fietto, Juliana Lopes Rangel Afonso, Luís Carlos Crocco |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Eduardo de Almeida Marques da Oliveira, Jamile Camargos de Figueiredo, Amanda Braga de Lima Júnior, Djalma de Souza Carneiro, Cláudia Martins Fietto, Juliana Lopes Rangel Afonso, Luís Carlos Crocco |
dc.subject.por.fl_str_mv |
Ecto-nucleotidase Adenosine Leishmania |
topic |
Ecto-nucleotidase Adenosine Leishmania |
description |
Leishmaniasis is a parasitic disease with a variety of clinical forms, which are related to the Leishmania species involved. In the murine model, Leishmania amazonensis causes chronic non-healing lesions in Leishmania braziliensis- or Leishmania major-resistant mouse strains. In this study, we investigated the involvement of the pathway of extracellular nucleotide hydrolysis, with special focus on the role of extracellular adenosine, in the establishment of Leishmania infection. Our results show that the more virulent parasitedL. Amazonensisdhydrolyzes higher amounts of ATP, ADP and AMP than the two other species, probably due to the higher expression of membrane NTPDase. Corroborating the idea that increased production of adenosine is important to lesion development and establishment of tissue parasitism, we observed that increased 50-nucleotidase activity in L. braziliensis or addition of adenosine at the moment of infection with this parasite resulted in an increase in lesion size and parasitism as well as a delay in lesion healing. Furthermore, inhibition of adenosine receptor A2B led to decreased lesion size and parasitism. Thus, our results suggest that the conversion of ATP, a molecule with pro-inflammatory activity, into adenosine, which possesses immunomodulatory properties, may contribute to the establishment of infection by Leishmania. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2012-09-19T15:38:09Z 2012-09-19T15:38:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, E. de A. M. da et al. Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Microbes and Infection, v. 10, n. 8, p. 850-857, jul. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1286457908001202>. Acesso em: 19 ago. 2012. 12864579 http://www.repositorio.ufop.br/handle/123456789/1404 |
identifier_str_mv |
SILVA, E. de A. M. da et al. Extracellular nucleotide metabolism in Leishmania : influence of adenosine in the establishment of infection. Microbes and Infection, v. 10, n. 8, p. 850-857, jul. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1286457908001202>. Acesso em: 19 ago. 2012. 12864579 |
url |
http://www.repositorio.ufop.br/handle/123456789/1404 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002865220780032 |