Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/3785 http://dx.doi.org/10.1590/S0074-02762008000200011 |
Resumo: | Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa pro¬tein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previ¬ously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil. |
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Oliveira, Karla Andrade deOliveira, Luciana Souza deDias, C. C. A.Silva Júnior, AbelardoLamëgo, Márcia Rogéria de AlmeidaAmalda, G.Bouyer, Donald H.Galvão, Márcio Antônio MoreiraSiqueira, Cláudio Lísias Mafra de2014-11-11T19:41:31Z2014-11-11T19:41:31Z2008OLIVEIRA, K. A. de et al. Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Memórias do Instituto Oswaldo Cruz, v. 103, p. 191-194, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011&lng=en&nrm=iso&tlng=en>. Acesso em: 03 set. 2014.0074-0276http://www.repositorio.ufop.br/handle/123456789/3785http://dx.doi.org/10.1590/S0074-02762008000200011Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa pro¬tein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previ¬ously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.Rickettsia felisRickettsia rickettsiiCtenocephalides felisBrazilian spotted feverTickMolecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Memórias do Instituto Oswaldo Cruz permite que o Repositório Institucional da Universidade Federal de Ouro Preto (UFOP) deposite uma cópia eletrônica dos artigos publicados por esse periódico em que ao menos um dos autores faça parte da comunidade cientifica da UFOP. Fonte: Licença concedida mediante prenchimento de formulário enviado no dia 12/12/2013.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3785/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_MolecularIdentificationRickettsia.pdfARTIGO_MolecularIdentificationRickettsia.pdfapplication/pdf361149http://www.repositorio.ufop.br/bitstream/123456789/3785/1/ARTIGO_MolecularIdentificationRickettsia.pdf82a20b55027deff04de0b6ab7fb1b81dMD51123456789/37852019-05-07 11:50:21.924oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-05-07T15:50:21Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
title |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
spellingShingle |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Oliveira, Karla Andrade de Rickettsia felis Rickettsia rickettsii Ctenocephalides felis Brazilian spotted fever Tick |
title_short |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
title_full |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
title_fullStr |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
title_full_unstemmed |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
title_sort |
Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. |
author |
Oliveira, Karla Andrade de |
author_facet |
Oliveira, Karla Andrade de Oliveira, Luciana Souza de Dias, C. C. A. Silva Júnior, Abelardo Lamëgo, Márcia Rogéria de Almeida Amalda, G. Bouyer, Donald H. Galvão, Márcio Antônio Moreira Siqueira, Cláudio Lísias Mafra de |
author_role |
author |
author2 |
Oliveira, Luciana Souza de Dias, C. C. A. Silva Júnior, Abelardo Lamëgo, Márcia Rogéria de Almeida Amalda, G. Bouyer, Donald H. Galvão, Márcio Antônio Moreira Siqueira, Cláudio Lísias Mafra de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Karla Andrade de Oliveira, Luciana Souza de Dias, C. C. A. Silva Júnior, Abelardo Lamëgo, Márcia Rogéria de Almeida Amalda, G. Bouyer, Donald H. Galvão, Márcio Antônio Moreira Siqueira, Cláudio Lísias Mafra de |
dc.subject.por.fl_str_mv |
Rickettsia felis Rickettsia rickettsii Ctenocephalides felis Brazilian spotted fever Tick |
topic |
Rickettsia felis Rickettsia rickettsii Ctenocephalides felis Brazilian spotted fever Tick |
description |
Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa pro¬tein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previ¬ously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2014-11-11T19:41:31Z |
dc.date.available.fl_str_mv |
2014-11-11T19:41:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, K. A. de et al. Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Memórias do Instituto Oswaldo Cruz, v. 103, p. 191-194, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011&lng=en&nrm=iso&tlng=en>. Acesso em: 03 set. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/3785 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762008000200011 |
identifier_str_mv |
OLIVEIRA, K. A. de et al. Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Memórias do Instituto Oswaldo Cruz, v. 103, p. 191-194, 2008. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011&lng=en&nrm=iso&tlng=en>. Acesso em: 03 set. 2014. 0074-0276 |
url |
http://www.repositorio.ufop.br/handle/123456789/3785 http://dx.doi.org/10.1590/S0074-02762008000200011 |
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eng |
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eng |
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UFOP |
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