PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/3771 |
Resumo: | The present work describes the preparation, characterization and labelling of conventional and surface-modified nanocapsules (NC) with 99mTc-HMPAO. The size, size distribution and homogeneity were determined by photon correlation spectroscopy (PCS) and zeta potential by laser doppler anemometry. The morphology and the structural organization were evaluated by atomic force microscopy (AFM). The stability and release profile of the NC were determined in vitro in plasma. The results showed that the use of methylene blue induces significant increase in the encapsulation efficiency of 99mTc-HMPAO, from 24.4 to 49.8% in PLANC and 22.37 to 52.93% in the case of PLA-PEGNC(P < 0.05) by improving the complex stabilization. The average diameter of NC calculated by PCS varied from 216 to 323 nm, while the average diameter determined by AFM varied from 238 to 426 nm. The AFM analysis of diameter/height ratios suggested a greater homogeneity of the surface-modified PLAPEG nanocapsules compared to PLA NC concerning their flattening properties. The in vitro release of the 99mTc-HMPAO in plasma medium was faster for the conventional PLA NC than for the surface-modified NC. For the latter, 60% of the radioactivity remained associated with NC, even after 12h of incubation. The results suggest that the surface-modified 99mTc-HMPAO-PLA PEG NCwas more stable against label leakage in the presence of proteins and could present better performance as radiotracer in vivo. |
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Pereira, Maira AlvesMosqueira, Vanessa Carla FurtadoVilela, José Mário CarneiroAndrade, Margareth SpanglerRamaldes, Gilson AndradeCardoso, Valbert Nascimento2014-11-11T18:17:34Z2014-11-11T18:17:34Z2008PEREIRA, M. A. et al. PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. European Journal of Pharmaceutical Sciences. V. 33, p. 42-51, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0928098707003636>. Acesso em: 20 ago. 2014.0928-0987http://www.repositorio.ufop.br/handle/123456789/3771The present work describes the preparation, characterization and labelling of conventional and surface-modified nanocapsules (NC) with 99mTc-HMPAO. The size, size distribution and homogeneity were determined by photon correlation spectroscopy (PCS) and zeta potential by laser doppler anemometry. The morphology and the structural organization were evaluated by atomic force microscopy (AFM). The stability and release profile of the NC were determined in vitro in plasma. The results showed that the use of methylene blue induces significant increase in the encapsulation efficiency of 99mTc-HMPAO, from 24.4 to 49.8% in PLANC and 22.37 to 52.93% in the case of PLA-PEGNC(P < 0.05) by improving the complex stabilization. The average diameter of NC calculated by PCS varied from 216 to 323 nm, while the average diameter determined by AFM varied from 238 to 426 nm. The AFM analysis of diameter/height ratios suggested a greater homogeneity of the surface-modified PLAPEG nanocapsules compared to PLA NC concerning their flattening properties. The in vitro release of the 99mTc-HMPAO in plasma medium was faster for the conventional PLA NC than for the surface-modified NC. For the latter, 60% of the radioactivity remained associated with NC, even after 12h of incubation. The results suggest that the surface-modified 99mTc-HMPAO-PLA PEG NCwas more stable against label leakage in the presence of proteins and could present better performance as radiotracer in vivo.NanocapsulesAtomic force microscopyPhysicochemical characterizationPhoton correlation spectroscopyRadiotracerPLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Advanced Drug Delivery Reviews concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3460320800963.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3771/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_PLA-PEGnanocapsules.pdfARTIGO_PLA-PEGnanocapsules.pdfapplication/pdf839442http://www.repositorio.ufop.br/bitstream/123456789/3771/1/ARTIGO_PLA-PEGnanocapsules.pdff30530d804da696e612169211f141cfaMD51123456789/37712019-05-07 10:41:38.784oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-05-07T14:41:38Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
title |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
spellingShingle |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. Pereira, Maira Alves Nanocapsules Atomic force microscopy Physicochemical characterization Photon correlation spectroscopy Radiotracer |
title_short |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
title_full |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
title_fullStr |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
title_full_unstemmed |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
title_sort |
PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. |
author |
Pereira, Maira Alves |
author_facet |
Pereira, Maira Alves Mosqueira, Vanessa Carla Furtado Vilela, José Mário Carneiro Andrade, Margareth Spangler Ramaldes, Gilson Andrade Cardoso, Valbert Nascimento |
author_role |
author |
author2 |
Mosqueira, Vanessa Carla Furtado Vilela, José Mário Carneiro Andrade, Margareth Spangler Ramaldes, Gilson Andrade Cardoso, Valbert Nascimento |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Maira Alves Mosqueira, Vanessa Carla Furtado Vilela, José Mário Carneiro Andrade, Margareth Spangler Ramaldes, Gilson Andrade Cardoso, Valbert Nascimento |
dc.subject.por.fl_str_mv |
Nanocapsules Atomic force microscopy Physicochemical characterization Photon correlation spectroscopy Radiotracer |
topic |
Nanocapsules Atomic force microscopy Physicochemical characterization Photon correlation spectroscopy Radiotracer |
description |
The present work describes the preparation, characterization and labelling of conventional and surface-modified nanocapsules (NC) with 99mTc-HMPAO. The size, size distribution and homogeneity were determined by photon correlation spectroscopy (PCS) and zeta potential by laser doppler anemometry. The morphology and the structural organization were evaluated by atomic force microscopy (AFM). The stability and release profile of the NC were determined in vitro in plasma. The results showed that the use of methylene blue induces significant increase in the encapsulation efficiency of 99mTc-HMPAO, from 24.4 to 49.8% in PLANC and 22.37 to 52.93% in the case of PLA-PEGNC(P < 0.05) by improving the complex stabilization. The average diameter of NC calculated by PCS varied from 216 to 323 nm, while the average diameter determined by AFM varied from 238 to 426 nm. The AFM analysis of diameter/height ratios suggested a greater homogeneity of the surface-modified PLAPEG nanocapsules compared to PLA NC concerning their flattening properties. The in vitro release of the 99mTc-HMPAO in plasma medium was faster for the conventional PLA NC than for the surface-modified NC. For the latter, 60% of the radioactivity remained associated with NC, even after 12h of incubation. The results suggest that the surface-modified 99mTc-HMPAO-PLA PEG NCwas more stable against label leakage in the presence of proteins and could present better performance as radiotracer in vivo. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2014-11-11T18:17:34Z |
dc.date.available.fl_str_mv |
2014-11-11T18:17:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, M. A. et al. PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. European Journal of Pharmaceutical Sciences. V. 33, p. 42-51, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0928098707003636>. Acesso em: 20 ago. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/3771 |
dc.identifier.issn.none.fl_str_mv |
0928-0987 |
identifier_str_mv |
PEREIRA, M. A. et al. PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. European Journal of Pharmaceutical Sciences. V. 33, p. 42-51, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0928098707003636>. Acesso em: 20 ago. 2014. 0928-0987 |
url |
http://www.repositorio.ufop.br/handle/123456789/3771 |
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eng |
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eng |
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UFOP |
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