Radiolabeling of cidofovir with technetium-99m and biodistribution studies

Detalhes bibliográficos
Autor(a) principal: Raquel Gregorio Arribada
Data de Publicação: 2020
Outros Autores: Nara Caroline Pereira, Valbert Nascimento Cardoso, Armando da Silva Cunha Júnior, André Luís Branco de Barros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1590/s2175-97902019000418511
http://hdl.handle.net/1843/47146
Resumo: Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.
id UFMG_639a17d071ff0879a93a81176b5e77a5
oai_identifier_str oai:repositorio.ufmg.br:1843/47146
network_acronym_str UFMG
network_name_str Repositório Institucional da UFMG
repository_id_str
spelling 2022-11-10T20:10:03Z2022-11-10T20:10:03Z2020-12-0956e1851119https://doi.org/10.1590/s2175-979020190004185112175-9790http://hdl.handle.net/1843/47146Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.A radiomarcação do cidofovir com tecnécio-99m (99mTc-CDV) é um procedimento inovador que permite o monitoramento em tempo real do medicamento. Os ensaios foram realizados in vitro, mostrando alta estabilidade do radiomarcador em 24 h. A depuração sanguínea, estudos de biodistribuição e imagens cintilográficas foram realizados em camundongos saudáveis para avaliar o perfil da droga in vivo. O 99mTc-CDV apresentou tempo de circulação sanguínea bifásico e captação renal significativa, indicando que o 99mTc-CDV é eliminado preferencialmente pela via renal. Bones também mostrou absorção importante ao longo do experimento. Em resumo, o cidofovir foi marcado com sucesso com tecnécio-99m e pode ser usado em estudos posteriores para rastrear a droga.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoengUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSBrazilian Journal of Pharmaceutical SciencesCidofovirTecnécioCintilografiaCidofovirRadiotracerTechnetium-99mBiodistributionScintigraphic imagingRadiolabeling of cidofovir with technetium-99m and biodistribution studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.revistas.usp.br/bjps/article/view/181834Raquel Gregorio ArribadaNara Caroline PereiraValbert Nascimento CardosoArmando da Silva Cunha JúniorAndré Luís Branco de Barrosapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/47146/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALRadiolabeling of cidofovir with technetium-99m and biodistribution studies.pdfRadiolabeling of cidofovir with technetium-99m and biodistribution studies.pdfapplication/pdf736274https://repositorio.ufmg.br/bitstream/1843/47146/2/Radiolabeling%20of%20cidofovir%20with%20technetium-99m%20and%20biodistribution%20studies.pdf8a57428b33dfff3e395888d001e66b5aMD521843/471462022-11-10 17:10:03.458oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-11-10T20:10:03Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Radiolabeling of cidofovir with technetium-99m and biodistribution studies
title Radiolabeling of cidofovir with technetium-99m and biodistribution studies
spellingShingle Radiolabeling of cidofovir with technetium-99m and biodistribution studies
Raquel Gregorio Arribada
Cidofovir
Radiotracer
Technetium-99m
Biodistribution
Scintigraphic imaging
Cidofovir
Tecnécio
Cintilografia
title_short Radiolabeling of cidofovir with technetium-99m and biodistribution studies
title_full Radiolabeling of cidofovir with technetium-99m and biodistribution studies
title_fullStr Radiolabeling of cidofovir with technetium-99m and biodistribution studies
title_full_unstemmed Radiolabeling of cidofovir with technetium-99m and biodistribution studies
title_sort Radiolabeling of cidofovir with technetium-99m and biodistribution studies
author Raquel Gregorio Arribada
author_facet Raquel Gregorio Arribada
Nara Caroline Pereira
Valbert Nascimento Cardoso
Armando da Silva Cunha Júnior
André Luís Branco de Barros
author_role author
author2 Nara Caroline Pereira
Valbert Nascimento Cardoso
Armando da Silva Cunha Júnior
André Luís Branco de Barros
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Raquel Gregorio Arribada
Nara Caroline Pereira
Valbert Nascimento Cardoso
Armando da Silva Cunha Júnior
André Luís Branco de Barros
dc.subject.por.fl_str_mv Cidofovir
Radiotracer
Technetium-99m
Biodistribution
Scintigraphic imaging
topic Cidofovir
Radiotracer
Technetium-99m
Biodistribution
Scintigraphic imaging
Cidofovir
Tecnécio
Cintilografia
dc.subject.other.pt_BR.fl_str_mv Cidofovir
Tecnécio
Cintilografia
description Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.
publishDate 2020
dc.date.issued.fl_str_mv 2020-12-09
dc.date.accessioned.fl_str_mv 2022-11-10T20:10:03Z
dc.date.available.fl_str_mv 2022-11-10T20:10:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/47146
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1590/s2175-97902019000418511
dc.identifier.issn.pt_BR.fl_str_mv 2175-9790
url https://doi.org/10.1590/s2175-97902019000418511
http://hdl.handle.net/1843/47146
identifier_str_mv 2175-9790
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Pharmaceutical Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/47146/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/47146/2/Radiolabeling%20of%20cidofovir%20with%20technetium-99m%20and%20biodistribution%20studies.pdf
bitstream.checksum.fl_str_mv fa505098d172de0bc8864fc1287ffe22
8a57428b33dfff3e395888d001e66b5a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv
_version_ 1801676794737197056

Registros relacionados