A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.

• Autor(a) principal: Santana, Marcos Aurélio de
• Data de Publicação: 2008
• Outros Autores: Santos, Alexandre Martins Costa, Oliveira, Marcelo Eurípedes, Oliveira, Jamil Silvano de, Babá, Élio Hideo, Santoro, Marcelo Matos, Andrade, Milton Hércules Guerra de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4576; https://doi.org/10.1016/j.ijbiomac.2008.07.011
• topic: Macrotyloma axillare; Lectin; Purification; Antigen

The N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.