Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/10839 |
Resumo: | Introduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease. |
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Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.Chagas diseaseHeart transplantationIntroduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.2019-03-27T16:46:29Z2019-03-27T16:46:29Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAZEVEDO, P. H. R. et al. Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 4, p. 467-474, jul./ago. 2018. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467&lng=en&nrm=iso>. Acesso em: 21 fev. 2019.00378682http://www.repositorio.ufop.br/handle/123456789/10839A Revista da Sociedade Brasileira de Medicina Tropical permite o depósito das versões pré-print e pós-print de um artigo. Permite remixagem, adaptação e nova criação a partir da obra para fins não comerciais desde que seja atribuído o crédito ao autor (CC BY-NC). Fonte: Diadorim <http://diadorim.ibict.br/handle/1/127>. Acesso em: 10 fev. 2020.info:eu-repo/semantics/openAccessAzevedo, Paulo Hernane RabeloXavier, Marcelo Antônio PascoalSilva, Glenda Nicioli daCosta, Priscilla Almeida daCarneiro, Cláudia MartinsBrasileiro Filho, Geraldoengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2020-03-10T13:42:36Zoai:repositorio.ufop.br:123456789/10839Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-03-10T13:42:36Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
title |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
spellingShingle |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Azevedo, Paulo Hernane Rabelo Chagas disease Heart transplantation |
title_short |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
title_full |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
title_fullStr |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
title_full_unstemmed |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
title_sort |
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. |
author |
Azevedo, Paulo Hernane Rabelo |
author_facet |
Azevedo, Paulo Hernane Rabelo Xavier, Marcelo Antônio Pascoal Silva, Glenda Nicioli da Costa, Priscilla Almeida da Carneiro, Cláudia Martins Brasileiro Filho, Geraldo |
author_role |
author |
author2 |
Xavier, Marcelo Antônio Pascoal Silva, Glenda Nicioli da Costa, Priscilla Almeida da Carneiro, Cláudia Martins Brasileiro Filho, Geraldo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Azevedo, Paulo Hernane Rabelo Xavier, Marcelo Antônio Pascoal Silva, Glenda Nicioli da Costa, Priscilla Almeida da Carneiro, Cláudia Martins Brasileiro Filho, Geraldo |
dc.subject.por.fl_str_mv |
Chagas disease Heart transplantation |
topic |
Chagas disease Heart transplantation |
description |
Introduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2019-03-27T16:46:29Z 2019-03-27T16:46:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
AZEVEDO, P. H. R. et al. Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 4, p. 467-474, jul./ago. 2018. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467&lng=en&nrm=iso>. Acesso em: 21 fev. 2019. 00378682 http://www.repositorio.ufop.br/handle/123456789/10839 |
identifier_str_mv |
AZEVEDO, P. H. R. et al. Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 4, p. 467-474, jul./ago. 2018. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467&lng=en&nrm=iso>. Acesso em: 21 fev. 2019. 00378682 |
url |
http://www.repositorio.ufop.br/handle/123456789/10839 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002820711874560 |