Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.

Detalhes bibliográficos
Autor(a) principal: Azevedo, Paulo Hernane Rabelo
Data de Publicação: 2018
Outros Autores: Xavier, Marcelo Antônio Pascoal, Silva, Glenda Nicioli da, Costa, Priscilla Almeida da, Carneiro, Cláudia Martins, Brasileiro Filho, Geraldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/10839
Resumo: Introduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
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spelling Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.Chagas diseaseHeart transplantationIntroduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.2019-03-27T16:46:29Z2019-03-27T16:46:29Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAZEVEDO, P. H. R. et al. Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 4, p. 467-474, jul./ago. 2018. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467&lng=en&nrm=iso>. Acesso em: 21 fev. 2019.00378682http://www.repositorio.ufop.br/handle/123456789/10839A Revista da Sociedade Brasileira de Medicina Tropical permite o depósito das versões pré-print e pós-print de um artigo. Permite remixagem, adaptação e nova criação a partir da obra para fins não comerciais desde que seja atribuído o crédito ao autor (CC BY-NC). Fonte: Diadorim <http://diadorim.ibict.br/handle/1/127>. Acesso em: 10 fev. 2020.info:eu-repo/semantics/openAccessAzevedo, Paulo Hernane RabeloXavier, Marcelo Antônio PascoalSilva, Glenda Nicioli daCosta, Priscilla Almeida daCarneiro, Cláudia MartinsBrasileiro Filho, Geraldoengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2020-03-10T13:42:36Zoai:repositorio.ufop.br:123456789/10839Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-03-10T13:42:36Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
title Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
spellingShingle Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
Azevedo, Paulo Hernane Rabelo
Chagas disease
Heart transplantation
title_short Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
title_full Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
title_fullStr Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
title_full_unstemmed Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
title_sort Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection.
author Azevedo, Paulo Hernane Rabelo
author_facet Azevedo, Paulo Hernane Rabelo
Xavier, Marcelo Antônio Pascoal
Silva, Glenda Nicioli da
Costa, Priscilla Almeida da
Carneiro, Cláudia Martins
Brasileiro Filho, Geraldo
author_role author
author2 Xavier, Marcelo Antônio Pascoal
Silva, Glenda Nicioli da
Costa, Priscilla Almeida da
Carneiro, Cláudia Martins
Brasileiro Filho, Geraldo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Azevedo, Paulo Hernane Rabelo
Xavier, Marcelo Antônio Pascoal
Silva, Glenda Nicioli da
Costa, Priscilla Almeida da
Carneiro, Cláudia Martins
Brasileiro Filho, Geraldo
dc.subject.por.fl_str_mv Chagas disease
Heart transplantation
topic Chagas disease
Heart transplantation
description Introduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-03-27T16:46:29Z
2019-03-27T16:46:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv AZEVEDO, P. H. R. et al. Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 4, p. 467-474, jul./ago. 2018. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467&lng=en&nrm=iso>. Acesso em: 21 fev. 2019.
00378682
http://www.repositorio.ufop.br/handle/123456789/10839
identifier_str_mv AZEVEDO, P. H. R. et al. Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 4, p. 467-474, jul./ago. 2018. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467&lng=en&nrm=iso>. Acesso em: 21 fev. 2019.
00378682
url http://www.repositorio.ufop.br/handle/123456789/10839
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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