Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection

Detalhes bibliográficos
Autor(a) principal: Azevedo,Paulo Hernane Rabelo
Data de Publicação: 2018
Outros Autores: Xavier,Marcelo Antônio Pascoal, Silva,Glenda Nicioli da, Costa,Priscilla Almeida da, Carneiro,Cláudia Martins, Brasileiro Filho,Geraldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467
Resumo: Abstract INTRODUCTION: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. METHODS: We used 1) positive controls (PCs) - 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls - a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases - 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. RESULTS: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. CONCLUSIONS: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
id SBMT-1_efe0cfa715264d568ad446b00880f12a
oai_identifier_str oai:scielo:S0037-86822018000400467
network_acronym_str SBMT-1
network_name_str Revista da Sociedade Brasileira de Medicina Tropical
repository_id_str
spelling Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detectionChagas diseaseHeart transplantationImmunohistochemistryTrypanosoma cruziAbstract INTRODUCTION: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. METHODS: We used 1) positive controls (PCs) - 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls - a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases - 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. RESULTS: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. CONCLUSIONS: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.Sociedade Brasileira de Medicina Tropical - SBMT2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467Revista da Sociedade Brasileira de Medicina Tropical v.51 n.4 2018reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0011-2018info:eu-repo/semantics/openAccessAzevedo,Paulo Hernane RabeloXavier,Marcelo Antônio PascoalSilva,Glenda Nicioli daCosta,Priscilla Almeida daCarneiro,Cláudia MartinsBrasileiro Filho,Geraldoeng2018-08-16T00:00:00Zoai:scielo:S0037-86822018000400467Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2018-08-16T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
title Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
spellingShingle Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
Azevedo,Paulo Hernane Rabelo
Chagas disease
Heart transplantation
Immunohistochemistry
Trypanosoma cruzi
title_short Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
title_full Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
title_fullStr Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
title_full_unstemmed Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
title_sort Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
author Azevedo,Paulo Hernane Rabelo
author_facet Azevedo,Paulo Hernane Rabelo
Xavier,Marcelo Antônio Pascoal
Silva,Glenda Nicioli da
Costa,Priscilla Almeida da
Carneiro,Cláudia Martins
Brasileiro Filho,Geraldo
author_role author
author2 Xavier,Marcelo Antônio Pascoal
Silva,Glenda Nicioli da
Costa,Priscilla Almeida da
Carneiro,Cláudia Martins
Brasileiro Filho,Geraldo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Azevedo,Paulo Hernane Rabelo
Xavier,Marcelo Antônio Pascoal
Silva,Glenda Nicioli da
Costa,Priscilla Almeida da
Carneiro,Cláudia Martins
Brasileiro Filho,Geraldo
dc.subject.por.fl_str_mv Chagas disease
Heart transplantation
Immunohistochemistry
Trypanosoma cruzi
topic Chagas disease
Heart transplantation
Immunohistochemistry
Trypanosoma cruzi
description Abstract INTRODUCTION: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. METHODS: We used 1) positive controls (PCs) - 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls - a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases - 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. RESULTS: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. CONCLUSIONS: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400467
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0011-2018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.51 n.4 2018
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
_version_ 1752122161182539776