Ações de bactérias láticas de duas marcas comerciais de leites fermentados sobre o ganho de peso e parâmetros hematológicos e histopatológicos de ratos wistar fazendo uso de indometacina

• Autor(a) principal: Oliveira, Cybelle Pereira de
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/tede/4023
• topic: Leite fermentado; Bactérias láticas; Experimentação animal; Hematologia; Histopatologia; Fermented milk; Lactic acid bacteria; Animal experimentation; Hematology; Histopathology; CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS

Researches demonstrate that the consumption of fermented milk is beneficial to the health due to the presence of lactic acid bacteria and of the products of the metabolism produced by them during the fermentation of the milk. The objective of this work was to evaluate the action of the lactic bacterias of two commercial marks of fermented milks, one cultivated with Lactobacillus paracasei (L1) and other with Lactobacillus casei (L2), on the weight gain and hematologic and histopathologic parameters of rats Wistar making the indomethacin use. The choice of the drug based on the hypothesis of the existence of the protection of the digestive system and of the histology of the organs of the animals against the possible aggression of the nonsteroidal anti-inflammatory for the concomitant administration of the fermented milks. Three lots of each fermented milk were used, taking place the count of the viability of the lactic bacterias, gram coloration, catalase and morphologic identification. Simultaneously, 60 rats Wistar males with 90 days, consuming commercial ration and water ad libitum, were divided in 6 groups of 10, being the group LP added of L1; LP + D of L1 + drug; C (control) of water; D, of drug; LC, of L2; and LC + D, of L2 + drug. The animals received the fermented milks (5mL/Kg/day) and/or the drug indomethacin (2mg/Kg/day) for gavage for 40 days, enrolling the weekly weights. After the sacrifice, blood samples were collected for the accomplishment of hemograms and it was verified the weight of the kidneys, spleens and livers, taking place the histopathology of these organs and of the stomachs and intestines. The obtained results were treated with ANOVA, Tukey, Friedman, Duncan, Kruskall-wallis and Wilcoxon (p<0,05), being used the statistical package R. The strains of L1 and L2 resulted in catalase negative, gram positive and morphology of bacilli, presenting final counts of bacteria with superior values to 106 UFC. The fermented milks added to the diets of LP and LC and the addition of the drug to D didn't influence in the weekly weight gain of the animals, but LP+D and LC+D statistically differed of C and D, suggesting there to be interaction among drug-food. In the difference among the initial and final weight of the animals, the groups that received fermented milk resembled each other to the control, except the group LP whose weight gain was inferior; D also presented deficit of weight gain in relation to the group C. The diets didn't influence in the weights of the kidneys of the experimental groups, LP just presented significant difference in terms of spleen weight in relation to the other groups and the weights of the livers of the groups LP, D and LC+D differed in relation to the control group, where D resulted in superior weight of C and LP and LC+D obtained inferior weights to the control group. In the blood parameters, the groups didn't differ to each other in the red and white series, nor in the differential leucocyte count, if not verifying immunostimulatory effects; already in the counts of the platelets, some groups presented statistical difference, however the obtained results were inside of the allowed. The histopathological analysis had not evidenced histology alterations in the stomachs, livers, kidneys and spleens, meeting discreet infiltrated of lymphoid cells in the own sheet of the intestine of the experimental groups.