Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética

Detalhes bibliográficos
Autor(a) principal: Assis, Caroline Severo de
Data de Publicação: 2023
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/26999
Resumo: Diabetic Retinopathy is a microvascular complication of Diabetes Mellitus and is one of the main causes of blindness in the working-age population. Retinopathy is closely related to the control of glycemic targets, the duration of diabetes and other complex clinical and genetic aspects that are still not fully understood. Vitamin D, the adipokine Adiponectin and their respective genetic variants, namely VDR, ADIPOQ and ADIPOR1, play important roles in the context of Type 2 Diabetes Mellitus and its complications and, for this reason, are the subject of interest and study. The objective of this work was to analyze the association between the genotypes of the polymorphisms located in the VDR (rs1544410), Adiponectin (rs266729) and adiponectin receptor 1 (rs7539542) genes and Diabetic Retinopathy with the clinical stages of the disease, clinical, biochemical, anthropometric, physical activity and food consumption. Patients with type 2 Diabetes Mellitus, with at least 5 years of diagnosis were recruited and submitted to retinal examination for clinical classification (with or without retinopathy and disease stage) generating the following experimental groups: without retinopathy (n= 139), with retinopathy (n=221) the second group being subdivided into non-proliferative retinopathy (RDNP) n=144 and proliferative retinopathy (RDP) n=77. Biochemical parameters (blood glucose, glycated hemoglobin, total cholesterol, HDL cholesterol, triglycerides, creatinine), anthropometric data, food intake and physical activity were collected and molecular analyzes were performed to determine the genotypes of the polymorphisms of interest. The genotypic and allelic distribution of the polymorphisms studied did not differ between groups without retinopathy compared to those with retinopathy, RDNP and RDP for all genes. However, the presence of the b allele of rs1544410 (Bb+bb) of the VDR gene was associated with higher levels of fasting glucose (p=0.04) and use of insulin therapy (p=0.01) in the group of patients with retinopathy suggesting that it is indicative of a less effective glycemic control than in carriers of the BB genotype. With regard to adiponectin, the presence of the G allele of the rs266729 polymorphism was a risk factor for limb amputation (p=0.009), and the CC genotype for the rs7539542 polymorphism of the adiponectin receptor 1 was a protective factor against amputation (p=0.045). The results of this work suggest that the genotypes of the polymorphisms rs1544410, rs266729 and rs7539542 in the genes of the Vitamin D receptor, adiponectin and Adiponectin Receptor 1 influence glycemic control and the occurrence of amputation in a population in Northeast Brazil.
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spelling Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabéticaDiabetes mellitus - AmputaçãoRetinopatia diabéticaAdiponectinaPolimorfismo genéticoVitamina D - ReceptoresAlimentação habitual - ConsumoDiabetes mellitus - AmputationDiabetic retinopathyAdiponectinGenetic polymorphismVitamin D - ReceptorsUsual diet - ConsumptionCNPQ::CIENCIAS DA SAUDE::NUTRICAODiabetic Retinopathy is a microvascular complication of Diabetes Mellitus and is one of the main causes of blindness in the working-age population. Retinopathy is closely related to the control of glycemic targets, the duration of diabetes and other complex clinical and genetic aspects that are still not fully understood. Vitamin D, the adipokine Adiponectin and their respective genetic variants, namely VDR, ADIPOQ and ADIPOR1, play important roles in the context of Type 2 Diabetes Mellitus and its complications and, for this reason, are the subject of interest and study. The objective of this work was to analyze the association between the genotypes of the polymorphisms located in the VDR (rs1544410), Adiponectin (rs266729) and adiponectin receptor 1 (rs7539542) genes and Diabetic Retinopathy with the clinical stages of the disease, clinical, biochemical, anthropometric, physical activity and food consumption. Patients with type 2 Diabetes Mellitus, with at least 5 years of diagnosis were recruited and submitted to retinal examination for clinical classification (with or without retinopathy and disease stage) generating the following experimental groups: without retinopathy (n= 139), with retinopathy (n=221) the second group being subdivided into non-proliferative retinopathy (RDNP) n=144 and proliferative retinopathy (RDP) n=77. Biochemical parameters (blood glucose, glycated hemoglobin, total cholesterol, HDL cholesterol, triglycerides, creatinine), anthropometric data, food intake and physical activity were collected and molecular analyzes were performed to determine the genotypes of the polymorphisms of interest. The genotypic and allelic distribution of the polymorphisms studied did not differ between groups without retinopathy compared to those with retinopathy, RDNP and RDP for all genes. However, the presence of the b allele of rs1544410 (Bb+bb) of the VDR gene was associated with higher levels of fasting glucose (p=0.04) and use of insulin therapy (p=0.01) in the group of patients with retinopathy suggesting that it is indicative of a less effective glycemic control than in carriers of the BB genotype. With regard to adiponectin, the presence of the G allele of the rs266729 polymorphism was a risk factor for limb amputation (p=0.009), and the CC genotype for the rs7539542 polymorphism of the adiponectin receptor 1 was a protective factor against amputation (p=0.045). The results of this work suggest that the genotypes of the polymorphisms rs1544410, rs266729 and rs7539542 in the genes of the Vitamin D receptor, adiponectin and Adiponectin Receptor 1 influence glycemic control and the occurrence of amputation in a population in Northeast Brazil.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Apoio à Pesquisa do Estado da Paraíba - FAPESQPró-Reitoria de Pós-graduação da UFPB (PRPG/UFPB)A Retinopatia Diabética é uma complicação microvascular do Diabetes Mellitus e é uma das principais causas de cegueira na população em idade ativa. A Retinopatia está intimamente relacionada ao controle de metas glicêmicas, ao tempo de duração do diabetes e a outros aspectos clínicos e genéticos complexos e ainda não compreendidos em sua totalidade. A vitamina D, a adipocina Adiponectina e suas respectivas variantes genéticas, a saber, VDR, ADIPOQ e ADIPOR1, apresentam papéis importantes no contexto de Diabetes Mellitus tipo 2 e suas complicações e por esse motivo são alvo de interesse e estudo. O objetivo deste trabalho foi analisar a associação entre os genótipos dos polimorfismos localizados nos genes VDR (rs1544410), Adiponectina (rs266729) e receptor 1 da adiponectina (rs7539542) e Retinopatia Diabética com os estágios clínicos da doença, aspectos clínicos, bioquímicos, antropométricos, de atividade física e de consumo alimentar. Pacientes com Diabetes Mellitus tipo 2, com no mínimo 5 anos de diagnóstico foram recrutados e submetidos à exame da retina para classificação clínica (com ou sem retinopatia e o estágio da doença) gerando os seguintes grupos experimentais: sem retinopatia (n= 139), com retinopatia (n=221) estando o segundo grupo subdividido em Retinopatia não proliferativa (RDNP) n= 144 e Retinopatia proliferativa (RDP) n=77. Parâmetros bioquímicos (glicemia, hemoglobina glicada, colesterol total, HDL colesterol, triglicerídeos, creatinina), dados antropométricos, de consumo alimentar e atividade física foram coletados e análises moleculares foram realizadas para determinação dos genótipos dos polimorfismos de interesse. A distribuição genotípica e alélica dos polimorfismos estudados não diferiu entre os grupos sem retinopatia comparado a com retinopatia, RDNP e RDP para todos os genes. Contudo, a presença do alelo b do rs1544410 (Bb+bb) do gene VDR foi associado a maiores níveis de glicemia de jejum (p=0,04) e uso de insulinoterapia (p=0,01) no grupo de pacientes com retinopatia sugerindo ser indicativo de um controle glicêmico menos efetivo do que em portadores do genótipo BB. Com relação a adiponectina, a presença do alelo G do polimorfismo rs266729 foi um fator de risco para amputação de membros (p=0,009), e o genótipo CC para o polimorfismo rs7539542 do receptor 1 de adiponectina foi um fator de proteção contra a amputação (p=0,045). Os resultados desse trabalho permitem sugerir que os genótipos dos polimorfismos rs1544410, rs266729 e rs7539542 nos genes do receptor de Vitamina D, adiponectina e Receptor 1 de adiponectina influenciam o controle da glicemia e ocorrência de amputação em uma população no Nordeste do Brasil.Universidade Federal da ParaíbaBrasilCiências da NutriçãoPrograma de Pós-Graduação em Ciências da NutriçãoUFPBPersuhn, Darlene Camatihttp://lattes.cnpq.br/0123092798297250Assis, Caroline Severo de2023-05-22T18:35:17Z2023-03-302023-05-22T18:35:17Z2023-02-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttps://repositorio.ufpb.br/jspui/handle/123456789/26999porAttribution-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2023-05-23T11:22:34Zoai:repositorio.ufpb.br:123456789/26999Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2023-05-23T11:22:34Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
title Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
spellingShingle Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
Assis, Caroline Severo de
Diabetes mellitus - Amputação
Retinopatia diabética
Adiponectina
Polimorfismo genético
Vitamina D - Receptores
Alimentação habitual - Consumo
Diabetes mellitus - Amputation
Diabetic retinopathy
Adiponectin
Genetic polymorphism
Vitamin D - Receptors
Usual diet - Consumption
CNPQ::CIENCIAS DA SAUDE::NUTRICAO
title_short Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
title_full Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
title_fullStr Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
title_full_unstemmed Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
title_sort Análise de polimorfismos nos genes do receptor de vitamina D, adiponectina e receptor 1 de adiponectina na retinopatia diabética
author Assis, Caroline Severo de
author_facet Assis, Caroline Severo de
author_role author
dc.contributor.none.fl_str_mv Persuhn, Darlene Camati
http://lattes.cnpq.br/0123092798297250
dc.contributor.author.fl_str_mv Assis, Caroline Severo de
dc.subject.por.fl_str_mv Diabetes mellitus - Amputação
Retinopatia diabética
Adiponectina
Polimorfismo genético
Vitamina D - Receptores
Alimentação habitual - Consumo
Diabetes mellitus - Amputation
Diabetic retinopathy
Adiponectin
Genetic polymorphism
Vitamin D - Receptors
Usual diet - Consumption
CNPQ::CIENCIAS DA SAUDE::NUTRICAO
topic Diabetes mellitus - Amputação
Retinopatia diabética
Adiponectina
Polimorfismo genético
Vitamina D - Receptores
Alimentação habitual - Consumo
Diabetes mellitus - Amputation
Diabetic retinopathy
Adiponectin
Genetic polymorphism
Vitamin D - Receptors
Usual diet - Consumption
CNPQ::CIENCIAS DA SAUDE::NUTRICAO
description Diabetic Retinopathy is a microvascular complication of Diabetes Mellitus and is one of the main causes of blindness in the working-age population. Retinopathy is closely related to the control of glycemic targets, the duration of diabetes and other complex clinical and genetic aspects that are still not fully understood. Vitamin D, the adipokine Adiponectin and their respective genetic variants, namely VDR, ADIPOQ and ADIPOR1, play important roles in the context of Type 2 Diabetes Mellitus and its complications and, for this reason, are the subject of interest and study. The objective of this work was to analyze the association between the genotypes of the polymorphisms located in the VDR (rs1544410), Adiponectin (rs266729) and adiponectin receptor 1 (rs7539542) genes and Diabetic Retinopathy with the clinical stages of the disease, clinical, biochemical, anthropometric, physical activity and food consumption. Patients with type 2 Diabetes Mellitus, with at least 5 years of diagnosis were recruited and submitted to retinal examination for clinical classification (with or without retinopathy and disease stage) generating the following experimental groups: without retinopathy (n= 139), with retinopathy (n=221) the second group being subdivided into non-proliferative retinopathy (RDNP) n=144 and proliferative retinopathy (RDP) n=77. Biochemical parameters (blood glucose, glycated hemoglobin, total cholesterol, HDL cholesterol, triglycerides, creatinine), anthropometric data, food intake and physical activity were collected and molecular analyzes were performed to determine the genotypes of the polymorphisms of interest. The genotypic and allelic distribution of the polymorphisms studied did not differ between groups without retinopathy compared to those with retinopathy, RDNP and RDP for all genes. However, the presence of the b allele of rs1544410 (Bb+bb) of the VDR gene was associated with higher levels of fasting glucose (p=0.04) and use of insulin therapy (p=0.01) in the group of patients with retinopathy suggesting that it is indicative of a less effective glycemic control than in carriers of the BB genotype. With regard to adiponectin, the presence of the G allele of the rs266729 polymorphism was a risk factor for limb amputation (p=0.009), and the CC genotype for the rs7539542 polymorphism of the adiponectin receptor 1 was a protective factor against amputation (p=0.045). The results of this work suggest that the genotypes of the polymorphisms rs1544410, rs266729 and rs7539542 in the genes of the Vitamin D receptor, adiponectin and Adiponectin Receptor 1 influence glycemic control and the occurrence of amputation in a population in Northeast Brazil.
publishDate 2023
dc.date.none.fl_str_mv 2023-05-22T18:35:17Z
2023-03-30
2023-05-22T18:35:17Z
2023-02-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/26999
url https://repositorio.ufpb.br/jspui/handle/123456789/26999
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Ciências da Nutrição
Programa de Pós-Graduação em Ciências da Nutrição
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Ciências da Nutrição
Programa de Pós-Graduação em Ciências da Nutrição
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
instacron_str UFPB
institution UFPB
reponame_str Biblioteca Digital de Teses e Dissertações da UFPB
collection Biblioteca Digital de Teses e Dissertações da UFPB
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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