Criopreservação de sêmen de galos

Detalhes bibliográficos
Autor(a) principal: Laan, Guilherme Martino Van Der
Data de Publicação: 2007
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFPel - Guaiaca
Texto Completo: http://guaiaca.ufpel.edu.br/handle/123456789/1253
Resumo: Adding extenders to poultry semen is currently used in artificial insemination programs to optimize the management of genetically superior males. Fresh semen fertility usually declines after 1 hour of collection, raising the need to use diluents and low temperatures to store semen for longer periods. Low density lipoprotein (LDL), extracted from the egg yolk, is used as a component of various mammalian semen diluents, however its application on poultry semen has not been evaluated. Regarding cryopreservation, different methodologies have been developed during the past years. One alternative is to use dimethylacetamide (DMA) as a cryoprotectant. The best results with DMA are obtained when semen is subjected to ultra-fast freezing, in pellets, and to a fast thawing at 60ºC. The objective of this work was to establish protocols to preserve rooster sperm, focusing on the use of LDL as a component of the refrigeration diluent, and on DMA as a internal cryoprotectant for freezing. To reach these objectives, the effect of adding different levels of LDL liposomes to the cooling diluent, upon the quality characteristics of semen refrigerated at 5 °C, was evaluated. The quality of semen frozen with DMA, packed in straws or pellets, and thawed in three different temperatures was also evaluated. The results obtained allow to conclude that: adding 6% of LDL liposomes to the cooling diluent preserves the general sperm quality, suggesting that improvements on fertility could be obtained if a limit in lipoprotein supplementation is imposed; body temperature (40°C) is most suitable for thawing sperm frozem with DMA; and packing sperm in straws is more efficient than packing in pellets. This last observation is of great value, since storing semen in straws is more appropriate due to sanitary reasons, and more convenient for ejaculates identification, especially for field application of genetic banks.
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spelling 2014-08-20T13:32:51Z2008-04-172014-08-20T13:32:51Z2007-11-30LAAN, Guilherme Martino Van Der. Roosters sperm cryopreservation. 2007. 56 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007.http://guaiaca.ufpel.edu.br/handle/123456789/1253Adding extenders to poultry semen is currently used in artificial insemination programs to optimize the management of genetically superior males. Fresh semen fertility usually declines after 1 hour of collection, raising the need to use diluents and low temperatures to store semen for longer periods. Low density lipoprotein (LDL), extracted from the egg yolk, is used as a component of various mammalian semen diluents, however its application on poultry semen has not been evaluated. Regarding cryopreservation, different methodologies have been developed during the past years. One alternative is to use dimethylacetamide (DMA) as a cryoprotectant. The best results with DMA are obtained when semen is subjected to ultra-fast freezing, in pellets, and to a fast thawing at 60ºC. The objective of this work was to establish protocols to preserve rooster sperm, focusing on the use of LDL as a component of the refrigeration diluent, and on DMA as a internal cryoprotectant for freezing. To reach these objectives, the effect of adding different levels of LDL liposomes to the cooling diluent, upon the quality characteristics of semen refrigerated at 5 °C, was evaluated. The quality of semen frozen with DMA, packed in straws or pellets, and thawed in three different temperatures was also evaluated. The results obtained allow to conclude that: adding 6% of LDL liposomes to the cooling diluent preserves the general sperm quality, suggesting that improvements on fertility could be obtained if a limit in lipoprotein supplementation is imposed; body temperature (40°C) is most suitable for thawing sperm frozem with DMA; and packing sperm in straws is more efficient than packing in pellets. This last observation is of great value, since storing semen in straws is more appropriate due to sanitary reasons, and more convenient for ejaculates identification, especially for field application of genetic banks.A adição de diluentes ao sêmen de aves é uma prática rotineiramente empregada em programas de inseminação artificial para melhorar o manejo de machos geneticamente superiores. A fertilidade do sêmen fresco normalmente decai 1 hora após a coleta, por isso a necessidade do uso de diluentes e temperaturas hipotérmicas para o armazenamento do sêmen por períodos mais prolongados. A Lipoproteína de Baixa Densidade (LDL), extraída da gema de ovo, tem sido utilizada na composição de diversos diluentes de sêmen para mamíferos, porém, ainda não tinha sido avaliada a sua aplicação em diluentes de resfriamento para sêmen de aves. Em relação ao congelamento, diversos métodos foram desenvolvidos ao longo dos anos. Uma das alternativas é o uso da Dimetilacetamida (DMA) como crioprotetor. Os melhores resultados obtidos com DMA ocorrem quando o sêmen é submetido ao congelamento ultra-rápido na forma de pellets e a um rápido descongelamento à 60ºC. O objetivo deste trabalho foi o estabelecimento de protocolos de preservação de sêmen de galos, enfocando o uso de Lipoproteínas de Baixa Densidade (LDL) como um componente do diluente para resfriamento, e a Dimetilacetamida (DMA) como crioprotetor interno para o congelamento. Para isto, foi verificado o efeito da adição de diferentes níveis de lipossomas de LDL na composição do diluente de resfriamento, sobre as características de qualidade do sêmen resfriado à 5 °C. Também foi avaliada a qualidade do sêmen congelado utilizando DMA como crioprotetor, envasado em palhetas ou pellets, e descongelados em três diferentes temperaturas. Os resultados obtidos nestes estudos nos permitiram concluir que: a adição de lipossomas de LDL ao diluente de resfriamento mantém a qualidade geral dos espermatozóides quando adicionado na proporção de 6%; sugerindo que melhorias na fertilidade podem ser obtidas desde que seja respeitado um limite de adição desta lipoproteína ao diluente; a temperatura corporal (40°C) foi a mais apropriada para descongelamento de sêmen criopreservado com DMA; e ainda, o envase em palhetas é mais eficiente em relação ao pellet. Esta última observação é de grande valor, visto que o armazenamento do sêmen em palhetas é mais adequado por razões sanitárias, e mais conveniente para a identificação dos ejaculados, especialmente para a aplicação a campo de bancos genéticos.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaBiotechnologyCryopreservationDimethylacetamideResfrigerationLiposomesLDLDiluentsPoultryRoostersBiotecnologiaCriopreservaçãoDimetilacetamidaResfriamentoLipossomasLDLDiluentesAvesGalosCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMALCriopreservação de sêmen de galosRoosters sperm cryopreservationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783390T8Lucia Júnior, Thomazhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793234U8Corrêa, Márcio Nuneshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796653E9Deschamps, João CarlosLaan, Guilherme Martino Van Derinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_guilherme_van_der_laan.pdfapplication/pdf723829http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1253/1/dissertacao_guilherme_van_der_laan.pdf399e8e1ec5f5ec9b7f77f1ffa1d0af80MD51open accessTEXTdissertacao_guilherme_van_der_laan.pdf.txtdissertacao_guilherme_van_der_laan.pdf.txtExtracted Texttext/plain88579http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1253/2/dissertacao_guilherme_van_der_laan.pdf.txt61994bda8e22c45e2dd45ce8b9299be1MD52open accessTHUMBNAILdissertacao_guilherme_van_der_laan.pdf.jpgdissertacao_guilherme_van_der_laan.pdf.jpgGenerated Thumbnailimage/jpeg1742http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1253/3/dissertacao_guilherme_van_der_laan.pdf.jpgdbc8805730284991782be1b5855c3a4eMD53open access123456789/12532019-08-23 10:09:38.516open accessoai:guaiaca.ufpel.edu.br:123456789/1253Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:09:38Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false
dc.title.por.fl_str_mv Criopreservação de sêmen de galos
dc.title.alternative.eng.fl_str_mv Roosters sperm cryopreservation
title Criopreservação de sêmen de galos
spellingShingle Criopreservação de sêmen de galos
Laan, Guilherme Martino Van Der
Biotechnology
Cryopreservation
Dimethylacetamide
Resfrigeration
Liposomes
LDL
Diluents
Poultry
Roosters
Biotecnologia
Criopreservação
Dimetilacetamida
Resfriamento
Lipossomas
LDL
Diluentes
Aves
Galos
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMAL
title_short Criopreservação de sêmen de galos
title_full Criopreservação de sêmen de galos
title_fullStr Criopreservação de sêmen de galos
title_full_unstemmed Criopreservação de sêmen de galos
title_sort Criopreservação de sêmen de galos
author Laan, Guilherme Martino Van Der
author_facet Laan, Guilherme Martino Van Der
author_role author
dc.contributor.advisorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783390T8
dc.contributor.referees1.pt_BR.fl_str_mv Lucia Júnior, Thomaz
dc.contributor.referees1ID.por.fl_str_mv
dc.contributor.referees1Lattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793234U8
dc.contributor.referees2.pt_BR.fl_str_mv Corrêa, Márcio Nunes
dc.contributor.referees2ID.por.fl_str_mv
dc.contributor.referees2Lattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796653E9
dc.contributor.advisor1.fl_str_mv Deschamps, João Carlos
dc.contributor.author.fl_str_mv Laan, Guilherme Martino Van Der
contributor_str_mv Deschamps, João Carlos
dc.subject.eng.fl_str_mv Biotechnology
Cryopreservation
Dimethylacetamide
Resfrigeration
Liposomes
LDL
Diluents
Poultry
Roosters
topic Biotechnology
Cryopreservation
Dimethylacetamide
Resfrigeration
Liposomes
LDL
Diluents
Poultry
Roosters
Biotecnologia
Criopreservação
Dimetilacetamida
Resfriamento
Lipossomas
LDL
Diluentes
Aves
Galos
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMAL
dc.subject.por.fl_str_mv Biotecnologia
Criopreservação
Dimetilacetamida
Resfriamento
Lipossomas
LDL
Diluentes
Aves
Galos
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL::INSEMINACAO ARTIFICIAL ANIMAL
description Adding extenders to poultry semen is currently used in artificial insemination programs to optimize the management of genetically superior males. Fresh semen fertility usually declines after 1 hour of collection, raising the need to use diluents and low temperatures to store semen for longer periods. Low density lipoprotein (LDL), extracted from the egg yolk, is used as a component of various mammalian semen diluents, however its application on poultry semen has not been evaluated. Regarding cryopreservation, different methodologies have been developed during the past years. One alternative is to use dimethylacetamide (DMA) as a cryoprotectant. The best results with DMA are obtained when semen is subjected to ultra-fast freezing, in pellets, and to a fast thawing at 60ºC. The objective of this work was to establish protocols to preserve rooster sperm, focusing on the use of LDL as a component of the refrigeration diluent, and on DMA as a internal cryoprotectant for freezing. To reach these objectives, the effect of adding different levels of LDL liposomes to the cooling diluent, upon the quality characteristics of semen refrigerated at 5 °C, was evaluated. The quality of semen frozen with DMA, packed in straws or pellets, and thawed in three different temperatures was also evaluated. The results obtained allow to conclude that: adding 6% of LDL liposomes to the cooling diluent preserves the general sperm quality, suggesting that improvements on fertility could be obtained if a limit in lipoprotein supplementation is imposed; body temperature (40°C) is most suitable for thawing sperm frozem with DMA; and packing sperm in straws is more efficient than packing in pellets. This last observation is of great value, since storing semen in straws is more appropriate due to sanitary reasons, and more convenient for ejaculates identification, especially for field application of genetic banks.
publishDate 2007
dc.date.issued.fl_str_mv 2007-11-30
dc.date.available.fl_str_mv 2008-04-17
2014-08-20T13:32:51Z
dc.date.accessioned.fl_str_mv 2014-08-20T13:32:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv LAAN, Guilherme Martino Van Der. Roosters sperm cryopreservation. 2007. 56 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007.
dc.identifier.uri.fl_str_mv http://guaiaca.ufpel.edu.br/handle/123456789/1253
identifier_str_mv LAAN, Guilherme Martino Van Der. Roosters sperm cryopreservation. 2007. 56 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007.
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dc.publisher.initials.fl_str_mv UFPel
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