Hepatits C incidence and prevalence in kidney transplant patients
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Clinical and Biomedical Research |
Texto Completo: | https://seer.ufrgs.br/index.php/hcpa/article/view/124810 |
Resumo: | OBJECTIVE: To detect the prevalence and the seroconversion of the anti-HCV in renal transplants, while evaluating the presence of this antibody at the time of thetransplant, and during a 1-year follow-up, as well as the possibility of transmitting the disease to the recipient of the contaminated organ.PATIENTS AND METHODS: We investigated the prevalence of anti-HCV infection in 48 kidney transplant recipients, and also in their respective donors. Serumspecimens were collected from the organ recipients right before kidney transplant, and 6 and 12 months after transplant; serum specimens were collected from donors at the time of nephroctomy. The 192 samples were stored at -20º C. The anti-HCV tests used were commercial kits based on synthetic HCV peptides (UBI), enzygnost anti-HCV (Boehringer), and Abbot HCV EIA 2nd generation. In patients with a positive anti-hepatitis C UBI test, the presence of HCV-RNA was verified by polymerase chain reaction .RESULTS: Eleven of 40 patients had positive UBI results, and 12 of 48 had positive EIA anti-HCV results before the transplant. Sixteen patients were anti-HCV positive during the 1-year follow-up. Two patients became positive after 6 months, and one after 12 months. One of these patients was also HCV-RNA positive. No transplant recipient patient with positive anti-HCV before transplant seroconverted after 1 year. Fifty percent of the patients who received a kidney were HCV-RNA positive. Three of 40 donors indicated a positive anti-HCV antibody in the UBI test, and 4 of 48 donors indicated a positive anti-HCV antibody in the Boehringer and EIA tests. Two donors were HCV-RNA positive.CONCLUSIONS: The prevalence of anti-HCV before transplant was high, and the serconversion to positive was low during the follow-up; none of the anti-HCV positive patients seroconverted; the HCV-RNA positive patients did not change to negative after transplant, which indicates the persistence of viral replication even after immunosupression; anti-HCV positive donors, even in the presence of HCV-RNA, did not transmit the infection during 1 year after transplant. |
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Hepatits C incidence and prevalence in kidney transplant patientsPrevalência e incidência da hepatite C em pacientes submetidos a transplante renalKidney transplanthepatitis Cserological testsTransplantado renalhepatite Cavaliação serológicaOBJECTIVE: To detect the prevalence and the seroconversion of the anti-HCV in renal transplants, while evaluating the presence of this antibody at the time of thetransplant, and during a 1-year follow-up, as well as the possibility of transmitting the disease to the recipient of the contaminated organ.PATIENTS AND METHODS: We investigated the prevalence of anti-HCV infection in 48 kidney transplant recipients, and also in their respective donors. Serumspecimens were collected from the organ recipients right before kidney transplant, and 6 and 12 months after transplant; serum specimens were collected from donors at the time of nephroctomy. The 192 samples were stored at -20º C. The anti-HCV tests used were commercial kits based on synthetic HCV peptides (UBI), enzygnost anti-HCV (Boehringer), and Abbot HCV EIA 2nd generation. In patients with a positive anti-hepatitis C UBI test, the presence of HCV-RNA was verified by polymerase chain reaction .RESULTS: Eleven of 40 patients had positive UBI results, and 12 of 48 had positive EIA anti-HCV results before the transplant. Sixteen patients were anti-HCV positive during the 1-year follow-up. Two patients became positive after 6 months, and one after 12 months. One of these patients was also HCV-RNA positive. No transplant recipient patient with positive anti-HCV before transplant seroconverted after 1 year. Fifty percent of the patients who received a kidney were HCV-RNA positive. Three of 40 donors indicated a positive anti-HCV antibody in the UBI test, and 4 of 48 donors indicated a positive anti-HCV antibody in the Boehringer and EIA tests. Two donors were HCV-RNA positive.CONCLUSIONS: The prevalence of anti-HCV before transplant was high, and the serconversion to positive was low during the follow-up; none of the anti-HCV positive patients seroconverted; the HCV-RNA positive patients did not change to negative after transplant, which indicates the persistence of viral replication even after immunosupression; anti-HCV positive donors, even in the presence of HCV-RNA, did not transmit the infection during 1 year after transplant.OBJETIVO: Investigar a prevalência do anti-VHC em 48 receptores renais e seusrespectivos doadores.PACIENTES E MÉTODOS: Foi coletado sangue dos receptores pré-transplante, 6meses e 1 ano pós-transplante; e dos doadores, no momento da nefrectomia. As192 amostras foram conservadas a -20 °C. Os testes anti-VHC utilizados forampeptídeos sintéticos (UBI) e ELISA de segunda geração (Abbott). Nos pacientescom positividade ao anti-VHC pelo teste UBI, foi pesquisado o VHC-ARN por reaçãoem cadeia da polimerase.RESULTADOS: Onze de 40 receptores foram anti-VHC positivos pelo teste da UBIe 12 de 48 pelo teste da Abbott pré-transplante. Dezesseis pacientes apresentarampositividade ao anti-VHC no período de 1 ano pós-operatório. Dois positivaram aos6 meses e um em 1 ano. Um deles apresentou positividade também ao VHC-ARN.Nenhum paciente anti-VHC positivo seroconverteu com 1 ano de seguimento.Verificou-se a presença do VHC-ARN em 50% dos receptores renais. Três de 40doadores foram anti-VHC positivos pelo teste UBI e 4 de 48 pelo teste Abbott. Doisdoadores apresentaram positividade ao VHC-ARN.CONCLUSÕES: 1) A prevalência do anti-VHC pré-transplante foi alta, porém aseroconversão para anti-VHC positivo no seguimento de 1 ano foi baixa; 2) nenhumpaciente anti-VHC positivo seroconverteu; 3) houve manutenção da positividadeao VHC-ARN demonstrando persistência da replicação viral apesar daimunossupressão; 4) os doadores anti-VHC positivos, mesmo com a presença doVHC-ARN não transmitiram a infecção através do enxerto renal no seguimento de1 ano pós-operatório.HCPA/FAMED/UFRGS2022-06-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/124810Clinical & Biomedical Research; Vol. 18 No. 3 (1998): Periodical HCPAClinical and Biomedical Research; v. 18 n. 3 (1998): Revista HCPA2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSporhttps://seer.ufrgs.br/index.php/hcpa/article/view/124810/84976http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessZanotelli, Maria Lucia R. da Silveira, ThemisM. Correa, Maria C.Chersky, Marisa P.C. Cantisani, Guido 2022-09-16T16:32:29Zoai:seer.ufrgs.br:article/124810Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2022-09-16T16:32:29Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.none.fl_str_mv |
Hepatits C incidence and prevalence in kidney transplant patients Prevalência e incidência da hepatite C em pacientes submetidos a transplante renal |
title |
Hepatits C incidence and prevalence in kidney transplant patients |
spellingShingle |
Hepatits C incidence and prevalence in kidney transplant patients Zanotelli, Maria Lucia Kidney transplant hepatitis C serological tests Transplantado renal hepatite C avaliação serológica |
title_short |
Hepatits C incidence and prevalence in kidney transplant patients |
title_full |
Hepatits C incidence and prevalence in kidney transplant patients |
title_fullStr |
Hepatits C incidence and prevalence in kidney transplant patients |
title_full_unstemmed |
Hepatits C incidence and prevalence in kidney transplant patients |
title_sort |
Hepatits C incidence and prevalence in kidney transplant patients |
author |
Zanotelli, Maria Lucia |
author_facet |
Zanotelli, Maria Lucia R. da Silveira, Themis M. Correa, Maria C. Chersky, Marisa P.C. Cantisani, Guido |
author_role |
author |
author2 |
R. da Silveira, Themis M. Correa, Maria C. Chersky, Marisa P.C. Cantisani, Guido |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Zanotelli, Maria Lucia R. da Silveira, Themis M. Correa, Maria C. Chersky, Marisa P.C. Cantisani, Guido |
dc.subject.por.fl_str_mv |
Kidney transplant hepatitis C serological tests Transplantado renal hepatite C avaliação serológica |
topic |
Kidney transplant hepatitis C serological tests Transplantado renal hepatite C avaliação serológica |
description |
OBJECTIVE: To detect the prevalence and the seroconversion of the anti-HCV in renal transplants, while evaluating the presence of this antibody at the time of thetransplant, and during a 1-year follow-up, as well as the possibility of transmitting the disease to the recipient of the contaminated organ.PATIENTS AND METHODS: We investigated the prevalence of anti-HCV infection in 48 kidney transplant recipients, and also in their respective donors. Serumspecimens were collected from the organ recipients right before kidney transplant, and 6 and 12 months after transplant; serum specimens were collected from donors at the time of nephroctomy. The 192 samples were stored at -20º C. The anti-HCV tests used were commercial kits based on synthetic HCV peptides (UBI), enzygnost anti-HCV (Boehringer), and Abbot HCV EIA 2nd generation. In patients with a positive anti-hepatitis C UBI test, the presence of HCV-RNA was verified by polymerase chain reaction .RESULTS: Eleven of 40 patients had positive UBI results, and 12 of 48 had positive EIA anti-HCV results before the transplant. Sixteen patients were anti-HCV positive during the 1-year follow-up. Two patients became positive after 6 months, and one after 12 months. One of these patients was also HCV-RNA positive. No transplant recipient patient with positive anti-HCV before transplant seroconverted after 1 year. Fifty percent of the patients who received a kidney were HCV-RNA positive. Three of 40 donors indicated a positive anti-HCV antibody in the UBI test, and 4 of 48 donors indicated a positive anti-HCV antibody in the Boehringer and EIA tests. Two donors were HCV-RNA positive.CONCLUSIONS: The prevalence of anti-HCV before transplant was high, and the serconversion to positive was low during the follow-up; none of the anti-HCV positive patients seroconverted; the HCV-RNA positive patients did not change to negative after transplant, which indicates the persistence of viral replication even after immunosupression; anti-HCV positive donors, even in the presence of HCV-RNA, did not transmit the infection during 1 year after transplant. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/124810 |
url |
https://seer.ufrgs.br/index.php/hcpa/article/view/124810 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/124810/84976 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
dc.source.none.fl_str_mv |
Clinical & Biomedical Research; Vol. 18 No. 3 (1998): Periodical HCPA Clinical and Biomedical Research; v. 18 n. 3 (1998): Revista HCPA 2357-9730 reponame:Clinical and Biomedical Research instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Clinical and Biomedical Research |
collection |
Clinical and Biomedical Research |
repository.name.fl_str_mv |
Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
||cbr@hcpa.edu.br |
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1799767056797663232 |