Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/131988 |
Resumo: | Erythrina species are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves of E. falcata and E. crista-galli. The hypotensive potential of E. falcata and the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. For in vivo protocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v.The results of total phenolic contents for E. falcata and E. cristagalli were 1.3193–1.4989mgGAE/mL for maceration and 0.8771–0.9506mgGAE/mL for infusion. In total flavonoids, the content was 7.7829–8.1976mg RE/g for maceration and 9.3471–10.4765 REmg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11-methoxyglucoerysodine, erysothiopine, 11-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed for E. falcata, which may be related to the route of -adrenergic receptors. |
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Mergulo, LiaraSantos, Marí CastroSant'Anna, Liane SantarianoCordeiro, Everson Willian FialhoMendez, Andreas Sebastian LoureiroMoreira, Cleci MenezesGarcia, Cassia VirginiaMiotto, Silvia Teresinha SfoggiaBatista, Luiz Alcides das Chagas2016-01-20T02:39:35Z20151741-427Xhttp://hdl.handle.net/10183/131988000981141Erythrina species are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves of E. falcata and E. crista-galli. The hypotensive potential of E. falcata and the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. For in vivo protocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v.The results of total phenolic contents for E. falcata and E. cristagalli were 1.3193–1.4989mgGAE/mL for maceration and 0.8771–0.9506mgGAE/mL for infusion. In total flavonoids, the content was 7.7829–8.1976mg RE/g for maceration and 9.3471–10.4765 REmg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11-methoxyglucoerysodine, erysothiopine, 11-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed for E. falcata, which may be related to the route of -adrenergic receptors.application/pdfengEvidence-based complementary and alternative medicine : eCAM. [New York]. Vol. 2015, (2015), ID 959081, 12 p.AlcalóidesQuímicaFarmáciaAlkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparationsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000981141.pdf000981141.pdfTexto completo (inglês)application/pdf2638087http://www.lume.ufrgs.br/bitstream/10183/131988/1/000981141.pdf5fd54522b1ec6ce232cf0506a24bdaf7MD51TEXT000981141.pdf.txt000981141.pdf.txtExtracted Texttext/plain48631http://www.lume.ufrgs.br/bitstream/10183/131988/2/000981141.pdf.txt9520ba6aeb16e5d5dd408904173a44caMD52THUMBNAIL000981141.pdf.jpg000981141.pdf.jpgGenerated Thumbnailimage/jpeg1756http://www.lume.ufrgs.br/bitstream/10183/131988/3/000981141.pdf.jpg2f2321e2ee1ab5e11787a93f8087774eMD5310183/1319882021-09-18 04:41:18.420144oai:www.lume.ufrgs.br:10183/131988Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-18T07:41:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
title |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
spellingShingle |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations Mergulo, Liara Alcalóides Química Farmácia |
title_short |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
title_full |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
title_fullStr |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
title_full_unstemmed |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
title_sort |
Alkaloids in erythrina by UPLC-ESI-MS and in vivo hypotensive potential of extractive preparations |
author |
Mergulo, Liara |
author_facet |
Mergulo, Liara Santos, Marí Castro Sant'Anna, Liane Santariano Cordeiro, Everson Willian Fialho Mendez, Andreas Sebastian Loureiro Moreira, Cleci Menezes Garcia, Cassia Virginia Miotto, Silvia Teresinha Sfoggia Batista, Luiz Alcides das Chagas |
author_role |
author |
author2 |
Santos, Marí Castro Sant'Anna, Liane Santariano Cordeiro, Everson Willian Fialho Mendez, Andreas Sebastian Loureiro Moreira, Cleci Menezes Garcia, Cassia Virginia Miotto, Silvia Teresinha Sfoggia Batista, Luiz Alcides das Chagas |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mergulo, Liara Santos, Marí Castro Sant'Anna, Liane Santariano Cordeiro, Everson Willian Fialho Mendez, Andreas Sebastian Loureiro Moreira, Cleci Menezes Garcia, Cassia Virginia Miotto, Silvia Teresinha Sfoggia Batista, Luiz Alcides das Chagas |
dc.subject.por.fl_str_mv |
Alcalóides Química Farmácia |
topic |
Alcalóides Química Farmácia |
description |
Erythrina species are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves of E. falcata and E. crista-galli. The hypotensive potential of E. falcata and the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. For in vivo protocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v.The results of total phenolic contents for E. falcata and E. cristagalli were 1.3193–1.4989mgGAE/mL for maceration and 0.8771–0.9506mgGAE/mL for infusion. In total flavonoids, the content was 7.7829–8.1976mg RE/g for maceration and 9.3471–10.4765 REmg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11-methoxyglucoerysodine, erysothiopine, 11-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed for E. falcata, which may be related to the route of -adrenergic receptors. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-01-20T02:39:35Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/131988 |
dc.identifier.issn.pt_BR.fl_str_mv |
1741-427X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000981141 |
identifier_str_mv |
1741-427X 000981141 |
url |
http://hdl.handle.net/10183/131988 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Evidence-based complementary and alternative medicine : eCAM. [New York]. Vol. 2015, (2015), ID 959081, 12 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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