Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/30563 |
Resumo: | We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis. |
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Maschmann, Raquel de AbreuVerza, MirelaSilva, Marcia Susana NunesSperhacke, Rosa DeaRibeiro, Marta OsórioSuffys, Philip NoelGomes, Harrison MagdinierTortoli, EnricoMarcelli, FiorellaZaha, ArnaldoRossetti, Maria Lucia Rosa2011-08-04T06:01:20Z20110074-0276http://hdl.handle.net/10183/30563000776671We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.application/pdfengMemorias do Instituto Oswaldo Cruz : an International Journal of Biological and Biomedical Research. Rio de Janeiro. Vol. 106, n. 2 (mar. 2011), p. 139-145Mycobacterium tuberculosisTuberculoseHibridizaçãoRifampicinaDrug resistanceRenverse dot blot hybridizationRifampinrpoBTuberculosisDetection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assayinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000776671.pdf.txt000776671.pdf.txtExtracted Texttext/plain34064http://www.lume.ufrgs.br/bitstream/10183/30563/2/000776671.pdf.txtaf5998b2d38cb8771fde08434a1195ebMD52ORIGINAL000776671.pdf000776671.pdfTexto completo (inglês)application/pdf449864http://www.lume.ufrgs.br/bitstream/10183/30563/1/000776671.pdff5226da900db449b01a57e643105d3e7MD51THUMBNAIL000776671.pdf.jpg000776671.pdf.jpgGenerated Thumbnailimage/jpeg2083http://www.lume.ufrgs.br/bitstream/10183/30563/3/000776671.pdf.jpgf4f992ece17c2882dd665db0e6e8ace8MD5310183/305632018-10-09 09:29:18.829oai:www.lume.ufrgs.br:10183/30563Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-09T12:29:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
spellingShingle |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay Maschmann, Raquel de Abreu Mycobacterium tuberculosis Tuberculose Hibridização Rifampicina Drug resistance Renverse dot blot hybridization Rifampin rpoB Tuberculosis |
title_short |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_full |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_fullStr |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_full_unstemmed |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
title_sort |
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay |
author |
Maschmann, Raquel de Abreu |
author_facet |
Maschmann, Raquel de Abreu Verza, Mirela Silva, Marcia Susana Nunes Sperhacke, Rosa Dea Ribeiro, Marta Osório Suffys, Philip Noel Gomes, Harrison Magdinier Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
author_role |
author |
author2 |
Verza, Mirela Silva, Marcia Susana Nunes Sperhacke, Rosa Dea Ribeiro, Marta Osório Suffys, Philip Noel Gomes, Harrison Magdinier Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maschmann, Raquel de Abreu Verza, Mirela Silva, Marcia Susana Nunes Sperhacke, Rosa Dea Ribeiro, Marta Osório Suffys, Philip Noel Gomes, Harrison Magdinier Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
dc.subject.por.fl_str_mv |
Mycobacterium tuberculosis Tuberculose Hibridização Rifampicina |
topic |
Mycobacterium tuberculosis Tuberculose Hibridização Rifampicina Drug resistance Renverse dot blot hybridization Rifampin rpoB Tuberculosis |
dc.subject.eng.fl_str_mv |
Drug resistance Renverse dot blot hybridization Rifampin rpoB Tuberculosis |
description |
We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis. |
publishDate |
2011 |
dc.date.accessioned.fl_str_mv |
2011-08-04T06:01:20Z |
dc.date.issued.fl_str_mv |
2011 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/30563 |
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0074-0276 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000776671 |
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http://hdl.handle.net/10183/30563 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Memorias do Instituto Oswaldo Cruz : an International Journal of Biological and Biomedical Research. Rio de Janeiro. Vol. 106, n. 2 (mar. 2011), p. 139-145 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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