Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis

• Autor(a) principal: Vieira, Patrícia de Brum
• Data de Publicação: 2015
• Outros Autores: Silva, Nícolas Luiz Feijó, Kist, Luiza Wilges, Oliveira, Giovanna Medeiros Tavares de, Bogo, Mauricio Reis, Carli, Geraldo Attilio de, Macedo, Alexandre José, Tasca, Tiana
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/147023
• topic: Farmácia; Trichomonas vaginalis; Iron; Nucleoside triphosphate diphosphohydrolase; Ecto-5’; Nucleotidase; Nucleotide hydrolysis

Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5’-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis. Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalis isolates from female and male patients were evaluated. Iron from different sources sustained T. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5’-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron.