Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/147023 |
Resumo: | Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5’-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis. Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalis isolates from female and male patients were evaluated. Iron from different sources sustained T. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5’-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron. |
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Vieira, Patrícia de BrumSilva, Nícolas Luiz FeijóKist, Luiza WilgesOliveira, Giovanna Medeiros Tavares deBogo, Mauricio ReisCarli, Geraldo Attilio deMacedo, Alexandre JoséTasca, Tiana2016-08-10T02:16:14Z20150074-0276http://hdl.handle.net/10183/147023000986733Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5’-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis. Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalis isolates from female and male patients were evaluated. Iron from different sources sustained T. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5’-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron.application/pdfengMemórias do Instituto Oswaldo Cruz. Vol. 110, n. 2 (2015), p. 201-208FarmáciaTrichomonas vaginalisIronNucleoside triphosphate diphosphohydrolaseEcto-5’NucleotidaseNucleotide hydrolysisIron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000986733.pdf000986733.pdfTexto completo (inglês)application/pdf770361http://www.lume.ufrgs.br/bitstream/10183/147023/1/000986733.pdfeb84497374e94a3031e3a2b26ea0f2bcMD51TEXT000986733.pdf.txt000986733.pdf.txtExtracted Texttext/plain32211http://www.lume.ufrgs.br/bitstream/10183/147023/2/000986733.pdf.txtceb8f4572722ee26e05d69b49edf7c82MD52THUMBNAIL000986733.pdf.jpg000986733.pdf.jpgGenerated Thumbnailimage/jpeg2184http://www.lume.ufrgs.br/bitstream/10183/147023/3/000986733.pdf.jpg6bf6d6937b418a64aff53331ca7f3435MD5310183/1470232024-03-20 04:48:18.552902oai:www.lume.ufrgs.br:10183/147023Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-03-20T07:48:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
title |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
spellingShingle |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis Vieira, Patrícia de Brum Farmácia Trichomonas vaginalis Iron Nucleoside triphosphate diphosphohydrolase Ecto-5’ Nucleotidase Nucleotide hydrolysis |
title_short |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
title_full |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
title_fullStr |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
title_full_unstemmed |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
title_sort |
Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis |
author |
Vieira, Patrícia de Brum |
author_facet |
Vieira, Patrícia de Brum Silva, Nícolas Luiz Feijó Kist, Luiza Wilges Oliveira, Giovanna Medeiros Tavares de Bogo, Mauricio Reis Carli, Geraldo Attilio de Macedo, Alexandre José Tasca, Tiana |
author_role |
author |
author2 |
Silva, Nícolas Luiz Feijó Kist, Luiza Wilges Oliveira, Giovanna Medeiros Tavares de Bogo, Mauricio Reis Carli, Geraldo Attilio de Macedo, Alexandre José Tasca, Tiana |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Patrícia de Brum Silva, Nícolas Luiz Feijó Kist, Luiza Wilges Oliveira, Giovanna Medeiros Tavares de Bogo, Mauricio Reis Carli, Geraldo Attilio de Macedo, Alexandre José Tasca, Tiana |
dc.subject.por.fl_str_mv |
Farmácia |
topic |
Farmácia Trichomonas vaginalis Iron Nucleoside triphosphate diphosphohydrolase Ecto-5’ Nucleotidase Nucleotide hydrolysis |
dc.subject.eng.fl_str_mv |
Trichomonas vaginalis Iron Nucleoside triphosphate diphosphohydrolase Ecto-5’ Nucleotidase Nucleotide hydrolysis |
description |
Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5’-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis. Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalis isolates from female and male patients were evaluated. Iron from different sources sustained T. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5’-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-08-10T02:16:14Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/147023 |
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0074-0276 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000986733 |
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url |
http://hdl.handle.net/10183/147023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Memórias do Instituto Oswaldo Cruz. Vol. 110, n. 2 (2015), p. 201-208 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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