Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101

Detalhes bibliográficos
Autor(a) principal: Borges, Caroline Dellinghausen
Data de Publicação: 2008
Outros Autores: Vendruscolo, Claire Tondo, Moreira, Angelita da Silveira, Ayub, Marco Antônio Záchia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/71711
Resumo: Production, viscosity, and chemical composition of xanthan synthesized by bacterium Xanthomonas campestris pv pruni strain 101 were evaluated in bioreactor systems. During the process, the volumetric oxygen mass transfer coefficient (kLa) and the biomass were determined and the pH was monitored. The cultures were grown in a 3 l bioreactor, with aeration and agitation varying as follows: conditions (A) 300 rpm, 3 vvm and (B) 200 rpm, 2 vvm, at 28 °C. Our results showed that gum production was dependent on kLa, with a maximum yield of 8.15 g/l at 300 rpm, 3 vvm, 54 h of fermentation, kLa 21.4/h, while biomass was not affected. All aqueous solutions of 3% (w/v) xanthans synthesized showed a pseudoplastic behavior. The highest viscosity was reached under the strongest aeration/agitation conditions. All xanthan samples contained glucose, mannose, rhamnose, and glucuronic acid as their main components. The highest agitation and aeration rates used under condition A (300 rpm and 3 vvm) favorably influenced the yield and viscosity of the xanthan produced by bacterium X. campestris pv pruni 101 at different fermentation times.
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spelling Borges, Caroline DellinghausenVendruscolo, Claire TondoMoreira, Angelita da SilveiraAyub, Marco Antônio Záchia2013-05-21T01:50:02Z20080325-7541http://hdl.handle.net/10183/71711000881343Production, viscosity, and chemical composition of xanthan synthesized by bacterium Xanthomonas campestris pv pruni strain 101 were evaluated in bioreactor systems. During the process, the volumetric oxygen mass transfer coefficient (kLa) and the biomass were determined and the pH was monitored. The cultures were grown in a 3 l bioreactor, with aeration and agitation varying as follows: conditions (A) 300 rpm, 3 vvm and (B) 200 rpm, 2 vvm, at 28 °C. Our results showed that gum production was dependent on kLa, with a maximum yield of 8.15 g/l at 300 rpm, 3 vvm, 54 h of fermentation, kLa 21.4/h, while biomass was not affected. All aqueous solutions of 3% (w/v) xanthans synthesized showed a pseudoplastic behavior. The highest viscosity was reached under the strongest aeration/agitation conditions. All xanthan samples contained glucose, mannose, rhamnose, and glucuronic acid as their main components. The highest agitation and aeration rates used under condition A (300 rpm and 3 vvm) favorably influenced the yield and viscosity of the xanthan produced by bacterium X. campestris pv pruni 101 at different fermentation times.Se evaluó la producción, viscosidad y composición química del xantano sintetizado por la bacteria Xanthomonas campestris pv. pruni cepa 101 en un fermentador. Durante el proceso se controló el pH y se determinaron el coeficiente de transferencia de masa de oxígeno (kLa) y la producción de masa celular seca. Los cultivos se realizaron en un fermentador de 3 l variando la aireación y la agitación, en las siguientes condiciones: (A) 300 rpm, 3 vvm y (B) 200 rpm, 2 vvm; a 28 °C. Nuestros resultados mostraron que la producción de goma fue dependiente del kLa, con un rendimiento máximo de 8,15 g/l a 300 rpm y 3 vvm a las 54 h de fermentación, kLa de 21,4/h, mientras que la producción de biomasa no se afectó. Todas las soluciones acuosas de xantano al 3% (m/v) sintetizadas presentaron comportamiento pseudoplástico. La mayor viscosidad se alcanzó en la condición de aireación/agitación más intensa. Todas las muestras de xantano contenían glucosa, manosa, ramnosa y ácido glucurónico como constituyentes principales. La mayor tasa de agitación y aireación utilizada en la condición A (300 rpm y 3 vvm) influyó favorablemente en el rendimiento y la viscosidad del xantano producido por la bacteria X. campestris pv. pruni 101 a diferentes tiempos de fermentación.application/pdfengRevista Argentina de Microbiología. Buenos Aires. Vol. 40, n. 2 (abr./jun. 2008), p. 81-85Goma xantanaXanthomonas campestrisXanthan gumAeration/agitationXanthomonas campestris pv pruniGoma xantanoAireación/agitaciónInfluence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101Influencia de la agitación y la aireación en la producción de xantano por Xanthomonas campestris pv. pruni cepa 101 Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000881343.pdf.txt000881343.pdf.txtExtracted Texttext/plain25921http://www.lume.ufrgs.br/bitstream/10183/71711/2/000881343.pdf.txtb016b2ba300aaee36e665f3738b5be11MD52ORIGINAL000881343.pdf000881343.pdfTexto completo (inglês)application/pdf81305http://www.lume.ufrgs.br/bitstream/10183/71711/1/000881343.pdf953aff0e7f371f55d8f2b4c05119c992MD51THUMBNAIL000881343.pdf.jpg000881343.pdf.jpgGenerated Thumbnailimage/jpeg1967http://www.lume.ufrgs.br/bitstream/10183/71711/3/000881343.pdf.jpg06baaa061ae6a30416067f458fbccf5fMD5310183/717112018-10-17 07:49:52.688oai:www.lume.ufrgs.br:10183/71711Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-17T10:49:52Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
dc.title.alternative.es.fl_str_mv Influencia de la agitación y la aireación en la producción de xantano por Xanthomonas campestris pv. pruni cepa 101
title Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
spellingShingle Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
Borges, Caroline Dellinghausen
Goma xantana
Xanthomonas campestris
Xanthan gum
Aeration/agitation
Xanthomonas campestris pv pruni
Goma xantano
Aireación/agitación
title_short Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
title_full Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
title_fullStr Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
title_full_unstemmed Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
title_sort Influence of agitation and aeration in xanthan production by Xanthomonas campestris pv pruni strain 101
author Borges, Caroline Dellinghausen
author_facet Borges, Caroline Dellinghausen
Vendruscolo, Claire Tondo
Moreira, Angelita da Silveira
Ayub, Marco Antônio Záchia
author_role author
author2 Vendruscolo, Claire Tondo
Moreira, Angelita da Silveira
Ayub, Marco Antônio Záchia
author2_role author
author
author
dc.contributor.author.fl_str_mv Borges, Caroline Dellinghausen
Vendruscolo, Claire Tondo
Moreira, Angelita da Silveira
Ayub, Marco Antônio Záchia
dc.subject.por.fl_str_mv Goma xantana
Xanthomonas campestris
topic Goma xantana
Xanthomonas campestris
Xanthan gum
Aeration/agitation
Xanthomonas campestris pv pruni
Goma xantano
Aireación/agitación
dc.subject.eng.fl_str_mv Xanthan gum
Aeration/agitation
dc.subject.spa.fl_str_mv Xanthomonas campestris pv pruni
Goma xantano
Aireación/agitación
description Production, viscosity, and chemical composition of xanthan synthesized by bacterium Xanthomonas campestris pv pruni strain 101 were evaluated in bioreactor systems. During the process, the volumetric oxygen mass transfer coefficient (kLa) and the biomass were determined and the pH was monitored. The cultures were grown in a 3 l bioreactor, with aeration and agitation varying as follows: conditions (A) 300 rpm, 3 vvm and (B) 200 rpm, 2 vvm, at 28 °C. Our results showed that gum production was dependent on kLa, with a maximum yield of 8.15 g/l at 300 rpm, 3 vvm, 54 h of fermentation, kLa 21.4/h, while biomass was not affected. All aqueous solutions of 3% (w/v) xanthans synthesized showed a pseudoplastic behavior. The highest viscosity was reached under the strongest aeration/agitation conditions. All xanthan samples contained glucose, mannose, rhamnose, and glucuronic acid as their main components. The highest agitation and aeration rates used under condition A (300 rpm and 3 vvm) favorably influenced the yield and viscosity of the xanthan produced by bacterium X. campestris pv pruni 101 at different fermentation times.
publishDate 2008
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dc.relation.ispartof.pt_BR.fl_str_mv Revista Argentina de Microbiología. Buenos Aires. Vol. 40, n. 2 (abr./jun. 2008), p. 81-85
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