Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/87969 |
Resumo: | Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were isolated from foods and fecal samples of victims of shigellosis outbreaks investigated by the Surveillance Service. Identification of the strains at specie level indicated that 71.1% of them were S. flexneri, 21.5% S. sonnei, and 0.7% S. dysenteriae. Ten strains (6.7%) were identified only as Shigella spp. An increasing occurrence of S. sonnei was observed after 2004. Most of the strains were resistant to streptomycin (88.6%), followed by ampicillin (84.6%), and sulfamethoxazole/trimethoprim (80.5 %). Resistant strains belonged to 73 patterns, and pattern A (resistance to ampicillin, sulfamethoxazole/trimethoprim, tetracycline, streptomycin, chloramphenicol, and intermediate resistance to kanamycin) grouped the largest number of isolates (n=36). PCR-ribotyping identified three banding patterns (SH1, SH2, and SH3). SH1 grouped all S. flexneri and SH2 grouped all S. sonnei. The S. dysenteriae strain belonged to group SH3. According to the results, several Shigella isolates shared the same PCR-rybotyping banding pattern and the same resistance profile, suggesting that closely related strains were responsible for the outbreaks. However, other molecular typing methods need to be applied to confirm the clonal relationship of these isolates. |
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Paula, Cheila Minéia Daniel deGeimba, Mercedes PassosAmaral, Patrícia Heidrich doTondo, Eduardo Cesar2014-02-28T01:50:45Z20101517-8382http://hdl.handle.net/10183/87969000903130Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were isolated from foods and fecal samples of victims of shigellosis outbreaks investigated by the Surveillance Service. Identification of the strains at specie level indicated that 71.1% of them were S. flexneri, 21.5% S. sonnei, and 0.7% S. dysenteriae. Ten strains (6.7%) were identified only as Shigella spp. An increasing occurrence of S. sonnei was observed after 2004. Most of the strains were resistant to streptomycin (88.6%), followed by ampicillin (84.6%), and sulfamethoxazole/trimethoprim (80.5 %). Resistant strains belonged to 73 patterns, and pattern A (resistance to ampicillin, sulfamethoxazole/trimethoprim, tetracycline, streptomycin, chloramphenicol, and intermediate resistance to kanamycin) grouped the largest number of isolates (n=36). PCR-ribotyping identified three banding patterns (SH1, SH2, and SH3). SH1 grouped all S. flexneri and SH2 grouped all S. sonnei. The S. dysenteriae strain belonged to group SH3. According to the results, several Shigella isolates shared the same PCR-rybotyping banding pattern and the same resistance profile, suggesting that closely related strains were responsible for the outbreaks. However, other molecular typing methods need to be applied to confirm the clonal relationship of these isolates.application/pdfengBrazilian journal of microbiology. São Paulo. Vol. 41, no. 4 (out./dez. 2010), p. 966-977Disenteria bacilarResistência antimicrobianaShigellaShigellaShigellosisantimicrobial,PCR-RibotypingRS/BrazilAntimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000903130.pdf000903130.pdfTexto completo (inglês)application/pdf833652http://www.lume.ufrgs.br/bitstream/10183/87969/1/000903130.pdf5dd1e0a1ae3058265106c2697c1ba59fMD51TEXT000903130.pdf.txt000903130.pdf.txtExtracted Texttext/plain42613http://www.lume.ufrgs.br/bitstream/10183/87969/2/000903130.pdf.txt2b4f95cf15e6bb49a4bf49ee30c74f40MD52THUMBNAIL000903130.pdf.jpg000903130.pdf.jpgGenerated Thumbnailimage/jpeg1761http://www.lume.ufrgs.br/bitstream/10183/87969/3/000903130.pdf.jpg6a71f9b406bc6aa8ebd2ae5ff306a917MD5310183/879692021-08-18 04:29:07.746099oai:www.lume.ufrgs.br:10183/87969Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:29:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
title |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
spellingShingle |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil Paula, Cheila Minéia Daniel de Disenteria bacilar Resistência antimicrobiana Shigella Shigella Shigellosis antimicrobial, PCR-Ribotyping RS/Brazil |
title_short |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
title_full |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
title_fullStr |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
title_full_unstemmed |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
title_sort |
Antimicrobial resistance and PCR-ribotyping of shigella responsible for foodborne outbreaks occurred in southern Brazil |
author |
Paula, Cheila Minéia Daniel de |
author_facet |
Paula, Cheila Minéia Daniel de Geimba, Mercedes Passos Amaral, Patrícia Heidrich do Tondo, Eduardo Cesar |
author_role |
author |
author2 |
Geimba, Mercedes Passos Amaral, Patrícia Heidrich do Tondo, Eduardo Cesar |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Paula, Cheila Minéia Daniel de Geimba, Mercedes Passos Amaral, Patrícia Heidrich do Tondo, Eduardo Cesar |
dc.subject.por.fl_str_mv |
Disenteria bacilar Resistência antimicrobiana Shigella |
topic |
Disenteria bacilar Resistência antimicrobiana Shigella Shigella Shigellosis antimicrobial, PCR-Ribotyping RS/Brazil |
dc.subject.eng.fl_str_mv |
Shigella Shigellosis antimicrobial, PCR-Ribotyping RS/Brazil |
description |
Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were isolated from foods and fecal samples of victims of shigellosis outbreaks investigated by the Surveillance Service. Identification of the strains at specie level indicated that 71.1% of them were S. flexneri, 21.5% S. sonnei, and 0.7% S. dysenteriae. Ten strains (6.7%) were identified only as Shigella spp. An increasing occurrence of S. sonnei was observed after 2004. Most of the strains were resistant to streptomycin (88.6%), followed by ampicillin (84.6%), and sulfamethoxazole/trimethoprim (80.5 %). Resistant strains belonged to 73 patterns, and pattern A (resistance to ampicillin, sulfamethoxazole/trimethoprim, tetracycline, streptomycin, chloramphenicol, and intermediate resistance to kanamycin) grouped the largest number of isolates (n=36). PCR-ribotyping identified three banding patterns (SH1, SH2, and SH3). SH1 grouped all S. flexneri and SH2 grouped all S. sonnei. The S. dysenteriae strain belonged to group SH3. According to the results, several Shigella isolates shared the same PCR-rybotyping banding pattern and the same resistance profile, suggesting that closely related strains were responsible for the outbreaks. However, other molecular typing methods need to be applied to confirm the clonal relationship of these isolates. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
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2014-02-28T01:50:45Z |
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http://hdl.handle.net/10183/87969 |
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1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000903130 |
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http://hdl.handle.net/10183/87969 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo. Vol. 41, no. 4 (out./dez. 2010), p. 966-977 |
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