Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/72914 |
Resumo: | This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions. |
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Bruxel, FernandaGuterres, Silvia StanisçuaskiTeixeira, Helder Ferreira2013-07-02T01:43:36Z20110100-4042http://hdl.handle.net/10183/72914000820772This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions.application/pdfengQuímica nova. São Paulo. Vol. 34, n. 9 (2011), p. 1643-1646NanoemulsõesEspectrofotometriaOligonucleotídeosValidação : Métodos analíticosAntimalarial oligonucleotidesNanoemulsionsUV analysisValidation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotideinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000820772.pdf000820772.pdfTexto completo (inglês)application/pdf291716http://www.lume.ufrgs.br/bitstream/10183/72914/1/000820772.pdf8d53308381510f5c32115a4826250feeMD51TEXT000820772.pdf.txt000820772.pdf.txtExtracted Texttext/plain20375http://www.lume.ufrgs.br/bitstream/10183/72914/2/000820772.pdf.txt32b7ec4c61e1a3626d33612d1a06083aMD52THUMBNAIL000820772.pdf.jpg000820772.pdf.jpgGenerated Thumbnailimage/jpeg1830http://www.lume.ufrgs.br/bitstream/10183/72914/3/000820772.pdf.jpg5643bc8be964ce89d839f7584171a64bMD5310183/729142018-10-15 08:04:57.483oai:www.lume.ufrgs.br:10183/72914Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-15T11:04:57Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
title |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
spellingShingle |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide Bruxel, Fernanda Nanoemulsões Espectrofotometria Oligonucleotídeos Validação : Métodos analíticos Antimalarial oligonucleotides Nanoemulsions UV analysis |
title_short |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
title_full |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
title_fullStr |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
title_full_unstemmed |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
title_sort |
Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide |
author |
Bruxel, Fernanda |
author_facet |
Bruxel, Fernanda Guterres, Silvia Stanisçuaski Teixeira, Helder Ferreira |
author_role |
author |
author2 |
Guterres, Silvia Stanisçuaski Teixeira, Helder Ferreira |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bruxel, Fernanda Guterres, Silvia Stanisçuaski Teixeira, Helder Ferreira |
dc.subject.por.fl_str_mv |
Nanoemulsões Espectrofotometria Oligonucleotídeos Validação : Métodos analíticos |
topic |
Nanoemulsões Espectrofotometria Oligonucleotídeos Validação : Métodos analíticos Antimalarial oligonucleotides Nanoemulsions UV analysis |
dc.subject.eng.fl_str_mv |
Antimalarial oligonucleotides Nanoemulsions UV analysis |
description |
This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2013-07-02T01:43:36Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/72914 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-4042 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000820772 |
identifier_str_mv |
0100-4042 000820772 |
url |
http://hdl.handle.net/10183/72914 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Química nova. São Paulo. Vol. 34, n. 9 (2011), p. 1643-1646 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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