Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/253346 |
Resumo: | Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether tran- scriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild-type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4-D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in REPRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly in- creased susceptibility to all applied herbicides, and imazethapyr in particular. Transcriptomic analysis of the imazethapyr-treated wild type and ros1 plants revealed a relation of the herbicide upregu- lated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid biosynthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1-dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment. |
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Markus, CatarinePecinka, AlesMerotto Junior, Aldo2023-01-02T05:09:15Z20211422-0067http://hdl.handle.net/10183/253346001153255Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether tran- scriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild-type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4-D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in REPRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly in- creased susceptibility to all applied herbicides, and imazethapyr in particular. Transcriptomic analysis of the imazethapyr-treated wild type and ros1 plants revealed a relation of the herbicide upregu- lated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid biosynthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1-dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment.application/pdfengInternational journal of molecular sciences. Basel. Vol. 22, no. 7 (2021), 3314, 20 p.Genética vegetalResistência à pesticidaErva daninhaArabidopsis thalianaEpigeneticsHerbicide resistanceChromatin mutantsROS1ImazethapyrGlyphosate2,4-DInsights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thalianaEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001153255.pdf.txt001153255.pdf.txtExtracted Texttext/plain87989http://www.lume.ufrgs.br/bitstream/10183/253346/2/001153255.pdf.txtf5a4b765993b97449377355bff5e92d2MD52ORIGINAL001153255.pdfTexto completo (inglês)application/pdf2641056http://www.lume.ufrgs.br/bitstream/10183/253346/1/001153255.pdf11223335a210d828f56ac1e96ea875caMD5110183/2533462023-01-04 06:10:23.754454oai:www.lume.ufrgs.br:10183/253346Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-04T08:10:23Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
title |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
spellingShingle |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana Markus, Catarine Genética vegetal Resistência à pesticida Erva daninha Arabidopsis thaliana Epigenetics Herbicide resistance Chromatin mutants ROS1 Imazethapyr Glyphosate 2,4-D |
title_short |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
title_full |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
title_fullStr |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
title_full_unstemmed |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
title_sort |
Insights into the role of transcriptional gene silencing in response to herbicide-Treatments in Arabidopsis thaliana |
author |
Markus, Catarine |
author_facet |
Markus, Catarine Pecinka, Ales Merotto Junior, Aldo |
author_role |
author |
author2 |
Pecinka, Ales Merotto Junior, Aldo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Markus, Catarine Pecinka, Ales Merotto Junior, Aldo |
dc.subject.por.fl_str_mv |
Genética vegetal Resistência à pesticida Erva daninha Arabidopsis thaliana |
topic |
Genética vegetal Resistência à pesticida Erva daninha Arabidopsis thaliana Epigenetics Herbicide resistance Chromatin mutants ROS1 Imazethapyr Glyphosate 2,4-D |
dc.subject.eng.fl_str_mv |
Epigenetics Herbicide resistance Chromatin mutants ROS1 Imazethapyr Glyphosate 2,4-D |
description |
Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether tran- scriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild-type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4-D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in REPRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly in- creased susceptibility to all applied herbicides, and imazethapyr in particular. Transcriptomic analysis of the imazethapyr-treated wild type and ros1 plants revealed a relation of the herbicide upregu- lated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid biosynthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1-dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2023-01-02T05:09:15Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/253346 |
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1422-0067 |
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001153255 |
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1422-0067 001153255 |
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http://hdl.handle.net/10183/253346 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
International journal of molecular sciences. Basel. Vol. 22, no. 7 (2021), 3314, 20 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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