Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/206376 |
Resumo: | Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 µM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 µM even in the presence of H2O2. In a concentration of 0.1 µM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 µM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide. |
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Costa, Flávia Póvoa daGomes, Bruna Puty SilvaNogueira, Lygia SegaMitre, Geovanni PereiraSantos, Sávio Monteiro dosTeixeira, Bruno José BritoKataoka, Maria Sueli da SilvaMartins, Manoela DominguesBarboza, Carlos Augusto GalvãoMonteiro, Marta ChagasRogez, Herve Louis GhislainOliveira, Edivaldo Herculano CorrêaLima, Rafael Rodrigues2020-03-04T04:17:31Z20202076-3921http://hdl.handle.net/10183/206376001110460Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 µM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 µM even in the presence of H2O2. In a concentration of 0.1 µM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 µM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide.application/pdfengAntioxidants. Basel. Vol. 9, no. 1 (Jan. 2020), p. 1-14, artigo 16EstilbenosPeróxido de hidrogênioLigamento periodontalEstresse oxidativoStilbenesHydrogen PeroxidePeriodontal LigamentOxidative StressViabilitypiceatannolPiceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative StressEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001110460.pdf.txt001110460.pdf.txtExtracted Texttext/plain53259http://www.lume.ufrgs.br/bitstream/10183/206376/2/001110460.pdf.txtc61e93e86310c8946cb7d750ff9d4352MD52ORIGINAL001110460.pdfTexto completo (inglês)application/pdf1944101http://www.lume.ufrgs.br/bitstream/10183/206376/1/001110460.pdf52f7659e494216a5034f0e82791c52c1MD5110183/2063762020-03-05 04:15:12.024303oai:www.lume.ufrgs.br:10183/206376Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-03-05T07:15:12Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
spellingShingle |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress Costa, Flávia Póvoa da Estilbenos Peróxido de hidrogênio Ligamento periodontal Estresse oxidativo Stilbenes Hydrogen Peroxide Periodontal Ligament Oxidative Stress Viability piceatannol |
title_short |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_full |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_fullStr |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_full_unstemmed |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
title_sort |
Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress |
author |
Costa, Flávia Póvoa da |
author_facet |
Costa, Flávia Póvoa da Gomes, Bruna Puty Silva Nogueira, Lygia Sega Mitre, Geovanni Pereira Santos, Sávio Monteiro dos Teixeira, Bruno José Brito Kataoka, Maria Sueli da Silva Martins, Manoela Domingues Barboza, Carlos Augusto Galvão Monteiro, Marta Chagas Rogez, Herve Louis Ghislain Oliveira, Edivaldo Herculano Corrêa Lima, Rafael Rodrigues |
author_role |
author |
author2 |
Gomes, Bruna Puty Silva Nogueira, Lygia Sega Mitre, Geovanni Pereira Santos, Sávio Monteiro dos Teixeira, Bruno José Brito Kataoka, Maria Sueli da Silva Martins, Manoela Domingues Barboza, Carlos Augusto Galvão Monteiro, Marta Chagas Rogez, Herve Louis Ghislain Oliveira, Edivaldo Herculano Corrêa Lima, Rafael Rodrigues |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Flávia Póvoa da Gomes, Bruna Puty Silva Nogueira, Lygia Sega Mitre, Geovanni Pereira Santos, Sávio Monteiro dos Teixeira, Bruno José Brito Kataoka, Maria Sueli da Silva Martins, Manoela Domingues Barboza, Carlos Augusto Galvão Monteiro, Marta Chagas Rogez, Herve Louis Ghislain Oliveira, Edivaldo Herculano Corrêa Lima, Rafael Rodrigues |
dc.subject.por.fl_str_mv |
Estilbenos Peróxido de hidrogênio Ligamento periodontal Estresse oxidativo |
topic |
Estilbenos Peróxido de hidrogênio Ligamento periodontal Estresse oxidativo Stilbenes Hydrogen Peroxide Periodontal Ligament Oxidative Stress Viability piceatannol |
dc.subject.eng.fl_str_mv |
Stilbenes Hydrogen Peroxide Periodontal Ligament Oxidative Stress Viability piceatannol |
description |
Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 µM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 µM even in the presence of H2O2. In a concentration of 0.1 µM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 µM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-03-04T04:17:31Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/206376 |
dc.identifier.issn.pt_BR.fl_str_mv |
2076-3921 |
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001110460 |
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2076-3921 001110460 |
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http://hdl.handle.net/10183/206376 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Antioxidants. Basel. Vol. 9, no. 1 (Jan. 2020), p. 1-14, artigo 16 |
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