Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225487 |
Resumo: | Macrophages are myeloid cells that play an essential role in inflammation and host defense, regulating immune responses and maintaining tissue homeostasis. Depending on the microenvironment, macrophages can polarize to two distinct phenotypes. The M1 phenotype is activated by IFN-c and bacterial products, and displays an inflammatory profile, while M2 macrophages are activated by IL-4 and tend to be anti-inflammatory or immunosupressive. It was observed that DnaK from Mycobacterium tuberculosis has immunosuppressive properties, inducing a tolerogenic phenotype in dendritic cells and MDSCs, contributing to graft acceptance and tumor growth. However, its role in macrophage polarization remains to be elucidated. We asked whether DnaK was able to modulate macrophage phenotype. Murine macrophages, derived from bone marrow, or from the peritoneum, were incubated with DnaK and their phenotype compared to M1 or M2 polarized macrophages. Treatment with DnaK leads macrophages to present higher arginase I activity, IL-10 production and FIZZ1 and Ym1 expression. Furthermore, DnaK increased surface levels of CD206. Importantly, DnaK-treated macrophages were able to promote tumor growth in an allogeneic melanoma model. Our results suggest that DnaK polarizes macrophages to the M2-like phenotype and could constitute a virulence factor and is an important immunomodulator of macrophage responses. |
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Lopes, Rafael LisboaBorges, Thiago de JesusAraujo, Jessica Fonseca dePinho, Nathana GustavoBergamin, Letícia ScusselBattastini, Ana Maria OliveiraMuraro, Stéfanie PrimonSouza, Ana Paula deZanin, Rafael FernandesBonorino, Cristina Beatriz Cazabuena2021-08-10T04:32:17Z20141932-6203http://hdl.handle.net/10183/225487000947331Macrophages are myeloid cells that play an essential role in inflammation and host defense, regulating immune responses and maintaining tissue homeostasis. Depending on the microenvironment, macrophages can polarize to two distinct phenotypes. The M1 phenotype is activated by IFN-c and bacterial products, and displays an inflammatory profile, while M2 macrophages are activated by IL-4 and tend to be anti-inflammatory or immunosupressive. It was observed that DnaK from Mycobacterium tuberculosis has immunosuppressive properties, inducing a tolerogenic phenotype in dendritic cells and MDSCs, contributing to graft acceptance and tumor growth. However, its role in macrophage polarization remains to be elucidated. We asked whether DnaK was able to modulate macrophage phenotype. Murine macrophages, derived from bone marrow, or from the peritoneum, were incubated with DnaK and their phenotype compared to M1 or M2 polarized macrophages. Treatment with DnaK leads macrophages to present higher arginase I activity, IL-10 production and FIZZ1 and Ym1 expression. Furthermore, DnaK increased surface levels of CD206. Importantly, DnaK-treated macrophages were able to promote tumor growth in an allogeneic melanoma model. Our results suggest that DnaK polarizes macrophages to the M2-like phenotype and could constitute a virulence factor and is an important immunomodulator of macrophage responses.application/pdfengPLoS ONE. San Francisco. Vol. 9, no. 11 (Nov. 2014), e113441, 16 p.MacrófagosMycobacterium tuberculosisFenótipoProteínas de choque térmico HSP70Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotypeEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000947331.pdf.txt000947331.pdf.txtExtracted Texttext/plain40567http://www.lume.ufrgs.br/bitstream/10183/225487/2/000947331.pdf.txt1c682e8834cc1a4cd762b31a1997cf71MD52ORIGINAL000947331.pdfTexto completo (inglês)application/pdf2550657http://www.lume.ufrgs.br/bitstream/10183/225487/1/000947331.pdf5142770ed0dfd23c51445bba159a786eMD5110183/2254872023-09-23 03:38:27.783878oai:www.lume.ufrgs.br:10183/225487Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-23T06:38:27Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
title |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
spellingShingle |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype Lopes, Rafael Lisboa Macrófagos Mycobacterium tuberculosis Fenótipo Proteínas de choque térmico HSP70 |
title_short |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
title_full |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
title_fullStr |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
title_full_unstemmed |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
title_sort |
Extracellular mycobacterial DnaK polarizes macrophages to the M2-like phenotype |
author |
Lopes, Rafael Lisboa |
author_facet |
Lopes, Rafael Lisboa Borges, Thiago de Jesus Araujo, Jessica Fonseca de Pinho, Nathana Gustavo Bergamin, Letícia Scussel Battastini, Ana Maria Oliveira Muraro, Stéfanie Primon Souza, Ana Paula de Zanin, Rafael Fernandes Bonorino, Cristina Beatriz Cazabuena |
author_role |
author |
author2 |
Borges, Thiago de Jesus Araujo, Jessica Fonseca de Pinho, Nathana Gustavo Bergamin, Letícia Scussel Battastini, Ana Maria Oliveira Muraro, Stéfanie Primon Souza, Ana Paula de Zanin, Rafael Fernandes Bonorino, Cristina Beatriz Cazabuena |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Rafael Lisboa Borges, Thiago de Jesus Araujo, Jessica Fonseca de Pinho, Nathana Gustavo Bergamin, Letícia Scussel Battastini, Ana Maria Oliveira Muraro, Stéfanie Primon Souza, Ana Paula de Zanin, Rafael Fernandes Bonorino, Cristina Beatriz Cazabuena |
dc.subject.por.fl_str_mv |
Macrófagos Mycobacterium tuberculosis Fenótipo Proteínas de choque térmico HSP70 |
topic |
Macrófagos Mycobacterium tuberculosis Fenótipo Proteínas de choque térmico HSP70 |
description |
Macrophages are myeloid cells that play an essential role in inflammation and host defense, regulating immune responses and maintaining tissue homeostasis. Depending on the microenvironment, macrophages can polarize to two distinct phenotypes. The M1 phenotype is activated by IFN-c and bacterial products, and displays an inflammatory profile, while M2 macrophages are activated by IL-4 and tend to be anti-inflammatory or immunosupressive. It was observed that DnaK from Mycobacterium tuberculosis has immunosuppressive properties, inducing a tolerogenic phenotype in dendritic cells and MDSCs, contributing to graft acceptance and tumor growth. However, its role in macrophage polarization remains to be elucidated. We asked whether DnaK was able to modulate macrophage phenotype. Murine macrophages, derived from bone marrow, or from the peritoneum, were incubated with DnaK and their phenotype compared to M1 or M2 polarized macrophages. Treatment with DnaK leads macrophages to present higher arginase I activity, IL-10 production and FIZZ1 and Ym1 expression. Furthermore, DnaK increased surface levels of CD206. Importantly, DnaK-treated macrophages were able to promote tumor growth in an allogeneic melanoma model. Our results suggest that DnaK polarizes macrophages to the M2-like phenotype and could constitute a virulence factor and is an important immunomodulator of macrophage responses. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2021-08-10T04:32:17Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10183/225487 |
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1932-6203 |
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000947331 |
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1932-6203 000947331 |
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http://hdl.handle.net/10183/225487 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 9, no. 11 (Nov. 2014), e113441, 16 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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