Serological evidence of orthopoxvirus infection in neotropical primates in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/250481 |
Resumo: | The genus Orthopoxvirus (OPXV) of the family Poxviridae comprises several viruses that are capable of infecting a wide range of hosts. One of the most widespread OPXVs is the Vaccinia virus (VACV), which circulates in zoonotic cycles in South America, especially in Brazil, infecting domestic and wild animals and humans and causing economic losses as well as impacting public health. Despite this, little is known about the presence and/or exposure of neotropical primates to orthopoxviruses in the country. In this study, we report the results of a search for evidence of OPVX infections in neotropical free-living primates in the state of Minas Gerais, southeast Brazil. The sera or liver tissues of 63 neotropical primates were examined through plaque reduction neutralization tests (PRNT) and real-time PCR. OPXV-specific neutralizing antibodies were detected in two sera (4.5%) from Callithrix penicillata, showing 55% and 85% reduction in plaque counts, evidencing their previous exposure to the virus. Both individuals were collected in urban areas. All real-time PCR assays were negative. This is the first time that evidence of OPXV exposure has been detected in C. penicillata, a species that usually lives at the interface between cities and forests, increasing risks of zoonotic transmissions through spillover/spillback events. In this way, studies on the circulation of OPXV in neotropical free-living primates are necessary, especially now, with the monkeypox virus being detected in new regions of the planet. |
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Campos, Fabrício SouzaFranco, Ana ClaudiaRoehe, Paulo MichelAbreu, Filipe Vieira Santos deOliveira, Danilo Bretas de2022-10-27T04:52:22Z20222076-0817http://hdl.handle.net/10183/250481001151981The genus Orthopoxvirus (OPXV) of the family Poxviridae comprises several viruses that are capable of infecting a wide range of hosts. One of the most widespread OPXVs is the Vaccinia virus (VACV), which circulates in zoonotic cycles in South America, especially in Brazil, infecting domestic and wild animals and humans and causing economic losses as well as impacting public health. Despite this, little is known about the presence and/or exposure of neotropical primates to orthopoxviruses in the country. In this study, we report the results of a search for evidence of OPVX infections in neotropical free-living primates in the state of Minas Gerais, southeast Brazil. The sera or liver tissues of 63 neotropical primates were examined through plaque reduction neutralization tests (PRNT) and real-time PCR. OPXV-specific neutralizing antibodies were detected in two sera (4.5%) from Callithrix penicillata, showing 55% and 85% reduction in plaque counts, evidencing their previous exposure to the virus. Both individuals were collected in urban areas. All real-time PCR assays were negative. This is the first time that evidence of OPXV exposure has been detected in C. penicillata, a species that usually lives at the interface between cities and forests, increasing risks of zoonotic transmissions through spillover/spillback events. In this way, studies on the circulation of OPXV in neotropical free-living primates are necessary, especially now, with the monkeypox virus being detected in new regions of the planet.application/pdfengPathogens. Basel. Vol. 11, no. 10 (Oct. 2022), 1167, 7 p.Infecções por poxviridaeOrthopoxvirusVírus VacciniaPoxviridaeNon-human primatesVaccinia virusPlaque reduction neutralization testSerological evidence of orthopoxvirus infection in neotropical primates in BrazilEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001151981.pdf.txt001151981.pdf.txtExtracted Texttext/plain33363http://www.lume.ufrgs.br/bitstream/10183/250481/2/001151981.pdf.txteb9024ae98aea8c69cf5c378154be718MD52ORIGINAL001151981.pdfTexto completo (inglês)application/pdf1203374http://www.lume.ufrgs.br/bitstream/10183/250481/1/001151981.pdf0e12eec6c22f8457d9fb7fea9dc2a042MD5110183/2504812022-10-28 04:47:45.782505oai:www.lume.ufrgs.br:10183/250481Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-10-28T07:47:45Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
title |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
spellingShingle |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil Campos, Fabrício Souza Infecções por poxviridae Orthopoxvirus Vírus Vaccinia Poxviridae Non-human primates Vaccinia virus Plaque reduction neutralization test |
title_short |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
title_full |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
title_fullStr |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
title_full_unstemmed |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
title_sort |
Serological evidence of orthopoxvirus infection in neotropical primates in Brazil |
author |
Campos, Fabrício Souza |
author_facet |
Campos, Fabrício Souza Franco, Ana Claudia Roehe, Paulo Michel Abreu, Filipe Vieira Santos de Oliveira, Danilo Bretas de |
author_role |
author |
author2 |
Franco, Ana Claudia Roehe, Paulo Michel Abreu, Filipe Vieira Santos de Oliveira, Danilo Bretas de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Campos, Fabrício Souza Franco, Ana Claudia Roehe, Paulo Michel Abreu, Filipe Vieira Santos de Oliveira, Danilo Bretas de |
dc.subject.por.fl_str_mv |
Infecções por poxviridae Orthopoxvirus Vírus Vaccinia |
topic |
Infecções por poxviridae Orthopoxvirus Vírus Vaccinia Poxviridae Non-human primates Vaccinia virus Plaque reduction neutralization test |
dc.subject.eng.fl_str_mv |
Poxviridae Non-human primates Vaccinia virus Plaque reduction neutralization test |
description |
The genus Orthopoxvirus (OPXV) of the family Poxviridae comprises several viruses that are capable of infecting a wide range of hosts. One of the most widespread OPXVs is the Vaccinia virus (VACV), which circulates in zoonotic cycles in South America, especially in Brazil, infecting domestic and wild animals and humans and causing economic losses as well as impacting public health. Despite this, little is known about the presence and/or exposure of neotropical primates to orthopoxviruses in the country. In this study, we report the results of a search for evidence of OPVX infections in neotropical free-living primates in the state of Minas Gerais, southeast Brazil. The sera or liver tissues of 63 neotropical primates were examined through plaque reduction neutralization tests (PRNT) and real-time PCR. OPXV-specific neutralizing antibodies were detected in two sera (4.5%) from Callithrix penicillata, showing 55% and 85% reduction in plaque counts, evidencing their previous exposure to the virus. Both individuals were collected in urban areas. All real-time PCR assays were negative. This is the first time that evidence of OPXV exposure has been detected in C. penicillata, a species that usually lives at the interface between cities and forests, increasing risks of zoonotic transmissions through spillover/spillback events. In this way, studies on the circulation of OPXV in neotropical free-living primates are necessary, especially now, with the monkeypox virus being detected in new regions of the planet. |
publishDate |
2022 |
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2022-10-27T04:52:22Z |
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2022 |
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http://hdl.handle.net/10183/250481 |
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2076-0817 |
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001151981 |
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http://hdl.handle.net/10183/250481 |
dc.language.iso.fl_str_mv |
eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Pathogens. Basel. Vol. 11, no. 10 (Oct. 2022), 1167, 7 p. |
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