Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/263607 |
Resumo: | Brain injuries such as trauma and stroke lead to glial scar formation by reactive astrocytes which produce and secret axonal outgrowth inhibitors. Chondroitin sulfate proteoglycans (CSPG) constitute a well-known class of extracellular matrix molecules produced at the glial scar and cause growth cone collapse. The CSPG glycosaminoglycan side chains composed of chondroitin sulfate (CS) are responsible for its inhibitory activity on neurite outgrowth and are dependent on RhoA activation. Here, we hypothesize that CSPG also impairs neural stem cell migration inhibiting their penetration into an injury site. We show that DCX+ neuroblasts do not penetrate a CSPG-rich injured area probably due to Nogo receptor activation and RhoA/ROCK signaling pathway as we demonstrate in vitro with neural stem cells cultured as neurospheres and pull-down for RhoA. Furthermore, CS-impaired cell migration in vitro induced the formation of large mature adhesions and altered cell protrusion dynamics. ROCK inhibition restored migration in vitro as well as decreased adhesion size. |
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Galindo, Layla TestaMundim, Mayara Terra Villela VieiraPinto, Agnes Araújo SardinhaChiarantin, Gabrielly Maria DenadaiAlmeida, Maíra Estanislau SoaresLamers, Marcelo LazzaronHorwitz, Alan RickSantos, Marinilce Fagundes dosPorcionatto, Marimélia2023-08-16T03:32:45Z20181559-1182http://hdl.handle.net/10183/263607001064747Brain injuries such as trauma and stroke lead to glial scar formation by reactive astrocytes which produce and secret axonal outgrowth inhibitors. Chondroitin sulfate proteoglycans (CSPG) constitute a well-known class of extracellular matrix molecules produced at the glial scar and cause growth cone collapse. The CSPG glycosaminoglycan side chains composed of chondroitin sulfate (CS) are responsible for its inhibitory activity on neurite outgrowth and are dependent on RhoA activation. Here, we hypothesize that CSPG also impairs neural stem cell migration inhibiting their penetration into an injury site. We show that DCX+ neuroblasts do not penetrate a CSPG-rich injured area probably due to Nogo receptor activation and RhoA/ROCK signaling pathway as we demonstrate in vitro with neural stem cells cultured as neurospheres and pull-down for RhoA. Furthermore, CS-impaired cell migration in vitro induced the formation of large mature adhesions and altered cell protrusion dynamics. ROCK inhibition restored migration in vitro as well as decreased adhesion size.application/pdfengMolecular neurobiology. New York. Vol. 55, no. 4 (Apr. 2018), p. 3185-3195Células tronco neuraisMovimento celularSulfatos de condroitinaLesões encefálicas traumáticasProteína rhoA de Ligação ao GTPNeural stem cellCell migrationChondroitin sulfateTraumatic brain injuryRhoARockChondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK ActivationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001064747.pdf.txt001064747.pdf.txtExtracted Texttext/plain48641http://www.lume.ufrgs.br/bitstream/10183/263607/2/001064747.pdf.txtb59df2d0763c6b2890770a60591c1a5dMD52ORIGINAL001064747.pdfTexto completo (inglês)application/pdf1761233http://www.lume.ufrgs.br/bitstream/10183/263607/1/001064747.pdf273e64f52f554f93f64ba226f8e0536cMD5110183/2636072023-08-17 03:35:25.939551oai:www.lume.ufrgs.br:10183/263607Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-17T06:35:25Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
title |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
spellingShingle |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation Galindo, Layla Testa Células tronco neurais Movimento celular Sulfatos de condroitina Lesões encefálicas traumáticas Proteína rhoA de Ligação ao GTP Neural stem cell Cell migration Chondroitin sulfate Traumatic brain injury RhoA Rock |
title_short |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
title_full |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
title_fullStr |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
title_full_unstemmed |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
title_sort |
Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation |
author |
Galindo, Layla Testa |
author_facet |
Galindo, Layla Testa Mundim, Mayara Terra Villela Vieira Pinto, Agnes Araújo Sardinha Chiarantin, Gabrielly Maria Denadai Almeida, Maíra Estanislau Soares Lamers, Marcelo Lazzaron Horwitz, Alan Rick Santos, Marinilce Fagundes dos Porcionatto, Marimélia |
author_role |
author |
author2 |
Mundim, Mayara Terra Villela Vieira Pinto, Agnes Araújo Sardinha Chiarantin, Gabrielly Maria Denadai Almeida, Maíra Estanislau Soares Lamers, Marcelo Lazzaron Horwitz, Alan Rick Santos, Marinilce Fagundes dos Porcionatto, Marimélia |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Galindo, Layla Testa Mundim, Mayara Terra Villela Vieira Pinto, Agnes Araújo Sardinha Chiarantin, Gabrielly Maria Denadai Almeida, Maíra Estanislau Soares Lamers, Marcelo Lazzaron Horwitz, Alan Rick Santos, Marinilce Fagundes dos Porcionatto, Marimélia |
dc.subject.por.fl_str_mv |
Células tronco neurais Movimento celular Sulfatos de condroitina Lesões encefálicas traumáticas Proteína rhoA de Ligação ao GTP |
topic |
Células tronco neurais Movimento celular Sulfatos de condroitina Lesões encefálicas traumáticas Proteína rhoA de Ligação ao GTP Neural stem cell Cell migration Chondroitin sulfate Traumatic brain injury RhoA Rock |
dc.subject.eng.fl_str_mv |
Neural stem cell Cell migration Chondroitin sulfate Traumatic brain injury RhoA Rock |
description |
Brain injuries such as trauma and stroke lead to glial scar formation by reactive astrocytes which produce and secret axonal outgrowth inhibitors. Chondroitin sulfate proteoglycans (CSPG) constitute a well-known class of extracellular matrix molecules produced at the glial scar and cause growth cone collapse. The CSPG glycosaminoglycan side chains composed of chondroitin sulfate (CS) are responsible for its inhibitory activity on neurite outgrowth and are dependent on RhoA activation. Here, we hypothesize that CSPG also impairs neural stem cell migration inhibiting their penetration into an injury site. We show that DCX+ neuroblasts do not penetrate a CSPG-rich injured area probably due to Nogo receptor activation and RhoA/ROCK signaling pathway as we demonstrate in vitro with neural stem cells cultured as neurospheres and pull-down for RhoA. Furthermore, CS-impaired cell migration in vitro induced the formation of large mature adhesions and altered cell protrusion dynamics. ROCK inhibition restored migration in vitro as well as decreased adhesion size. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2023-08-16T03:32:45Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/263607 |
dc.identifier.issn.pt_BR.fl_str_mv |
1559-1182 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001064747 |
identifier_str_mv |
1559-1182 001064747 |
url |
http://hdl.handle.net/10183/263607 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Molecular neurobiology. New York. Vol. 55, no. 4 (Apr. 2018), p. 3185-3195 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Repositório Institucional da UFRGS |
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Repositório Institucional da UFRGS |
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