Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain

Detalhes bibliográficos
Autor(a) principal: Morais Junior, Marcos Antonio de
Data de Publicação: 2003
Outros Autores: Santos, Jose Ferreira dos, Henriques, João Antonio Pêgas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/23381
Resumo: The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.
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spelling Morais Junior, Marcos Antonio deSantos, Jose Ferreira dosHenriques, João Antonio Pêgas2010-06-05T04:17:23Z20031415-4757http://hdl.handle.net/10183/23381000476059The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 26, no. 2 (June 2003), p.213-220GenéticaSaccharomyces cerevisiaeDNADNA repairGenetic recombinationRecA geneSporulationYeastExpression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid straininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000476059.pdf000476059.pdfTexto completo (inglês)application/pdf228261http://www.lume.ufrgs.br/bitstream/10183/23381/1/000476059.pdf8b023175a697fc3dbea90b560a19c904MD51TEXT000476059.pdf.txt000476059.pdf.txtExtracted Texttext/plain29074http://www.lume.ufrgs.br/bitstream/10183/23381/2/000476059.pdf.txteb8f01757214fdc5b2475b0a56e61a24MD52THUMBNAIL000476059.pdf.jpg000476059.pdf.jpgGenerated Thumbnailimage/jpeg1821http://www.lume.ufrgs.br/bitstream/10183/23381/3/000476059.pdf.jpga88ec6fc86c18041ef97f92ea4a69c4cMD5310183/233812019-06-20 02:36:22.229281oai:www.lume.ufrgs.br:10183/23381Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-06-20T05:36:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
title Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
spellingShingle Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
Morais Junior, Marcos Antonio de
Genética
Saccharomyces cerevisiae
DNA
DNA repair
Genetic recombination
RecA gene
Sporulation
Yeast
title_short Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
title_full Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
title_fullStr Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
title_full_unstemmed Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
title_sort Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
author Morais Junior, Marcos Antonio de
author_facet Morais Junior, Marcos Antonio de
Santos, Jose Ferreira dos
Henriques, João Antonio Pêgas
author_role author
author2 Santos, Jose Ferreira dos
Henriques, João Antonio Pêgas
author2_role author
author
dc.contributor.author.fl_str_mv Morais Junior, Marcos Antonio de
Santos, Jose Ferreira dos
Henriques, João Antonio Pêgas
dc.subject.por.fl_str_mv Genética
Saccharomyces cerevisiae
DNA
topic Genética
Saccharomyces cerevisiae
DNA
DNA repair
Genetic recombination
RecA gene
Sporulation
Yeast
dc.subject.eng.fl_str_mv DNA repair
Genetic recombination
RecA gene
Sporulation
Yeast
description The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2010-06-05T04:17:23Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/23381
dc.identifier.issn.pt_BR.fl_str_mv 1415-4757
dc.identifier.nrb.pt_BR.fl_str_mv 000476059
identifier_str_mv 1415-4757
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url http://hdl.handle.net/10183/23381
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Genetics and molecular biology. Ribeirão Preto. Vol. 26, no. 2 (June 2003), p.213-220
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