Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/211602 |
Resumo: | Muscle satellite cells (MSCs) are myogenic stem cells that play a critical role in post-hatch skeletal muscle growth and regeneration. Activation of regeneration pathways to repair muscle fiber damage requires both the proliferation and differentiation of different MSC populations as well as the function of resident phagocytic cells such as anti-inflammatory and pro-inflammatory macrophages. The Wooden Breast (WB) phenotype in broiler chickens is characterized by myofiber degeneration and extensive fibrosis. Previous work indicates that the resident MSC populations expressing the myogenic regulatory factors, Myf-5 and Pax7 are larger and more proliferative in broilers severely affected with WB vs. unaffected broilers. To further characterize the cellular and molecular changes occurring in WB-affected muscles, samples from pectoralis major (PM) muscles with varying severity of WB (WB score 0 = normal; 1 = mildly affected; 2 = severely affected) were collected at 25 and 43 days post-hatch (n = 8 per score per age) and processed for cryohistological and protein expression analyses. Collagen per field and densities of macrophages and MyoDC, Myf-5C, and Pax7C MSC populations were quantified on immunofluorescence-stained cryosections. Relative collagen protein expression was quantified by fluorescent Western Blotting. In both 25 and 43-daysold broilers, the proportion of collagen per field (P _ 0.021) and macrophage density (P _ 0.074) were greater in PM exhibiting severe WB compared with normal. At day 43, populations of MyoDC, Myf-5C:MyoDC MSC were larger and relative collagen protein expression was greater in WB-affected vs. unaffected broilers (P _ 0.05). Pax7C MSC relative to total cells was also increased as WB severity increased in 43-days-old broilers (P _ 0.05). Densities of Myf-5C (P = 0.092), MyoDC (P = 0.030), Myf5C:MyoDC (P = 0.046), and Myf-5C:MyoDC:Pax7C (P = 0.048) MSC were greater in WB score 1 birds compared with WB score 0 and 2 birds. Overall, alterations in the resident MSC and macrophage populations and collagen protein content were observed in WBaffected muscle. Further investigation will be required to determine how these changes in cell population kinetics and local autocrine and paracrine signaling are involved in the apparent dysregulation of muscle maintenance in WB-affected broilers. |
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Ferreira, Tamara ZinnKindlein, LírisFlees, Joshua J.Shortnacy, Lauren K.Vieira, Sérgio LuizNascimento, Vladimir Pinheiro doMeloche, Kathryn J.Starkey, Jessica D.2020-07-09T03:41:51Z20201664-042Xhttp://hdl.handle.net/10183/211602001115058Muscle satellite cells (MSCs) are myogenic stem cells that play a critical role in post-hatch skeletal muscle growth and regeneration. Activation of regeneration pathways to repair muscle fiber damage requires both the proliferation and differentiation of different MSC populations as well as the function of resident phagocytic cells such as anti-inflammatory and pro-inflammatory macrophages. The Wooden Breast (WB) phenotype in broiler chickens is characterized by myofiber degeneration and extensive fibrosis. Previous work indicates that the resident MSC populations expressing the myogenic regulatory factors, Myf-5 and Pax7 are larger and more proliferative in broilers severely affected with WB vs. unaffected broilers. To further characterize the cellular and molecular changes occurring in WB-affected muscles, samples from pectoralis major (PM) muscles with varying severity of WB (WB score 0 = normal; 1 = mildly affected; 2 = severely affected) were collected at 25 and 43 days post-hatch (n = 8 per score per age) and processed for cryohistological and protein expression analyses. Collagen per field and densities of macrophages and MyoDC, Myf-5C, and Pax7C MSC populations were quantified on immunofluorescence-stained cryosections. Relative collagen protein expression was quantified by fluorescent Western Blotting. In both 25 and 43-daysold broilers, the proportion of collagen per field (P _ 0.021) and macrophage density (P _ 0.074) were greater in PM exhibiting severe WB compared with normal. At day 43, populations of MyoDC, Myf-5C:MyoDC MSC were larger and relative collagen protein expression was greater in WB-affected vs. unaffected broilers (P _ 0.05). Pax7C MSC relative to total cells was also increased as WB severity increased in 43-days-old broilers (P _ 0.05). Densities of Myf-5C (P = 0.092), MyoDC (P = 0.030), Myf5C:MyoDC (P = 0.046), and Myf-5C:MyoDC:Pax7C (P = 0.048) MSC were greater in WB score 1 birds compared with WB score 0 and 2 birds. Overall, alterations in the resident MSC and macrophage populations and collagen protein content were observed in WBaffected muscle. Further investigation will be required to determine how these changes in cell population kinetics and local autocrine and paracrine signaling are involved in the apparent dysregulation of muscle maintenance in WB-affected broilers.application/pdfengFrontiers in Physiology. Lausanne. Vol. 11 (May 2020), 529, 11 p.Células satélites de músculo esqueléticoFatores de regulação miogênicaMacrófagosColágenoMiopatiaFrangos de corteWooden breastMuscle satellite cellMyogenic stem cellMacrophageCollagen infiltrationBroiler chickenCharacterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breastEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001115058.pdf.txt001115058.pdf.txtExtracted Texttext/plain51282http://www.lume.ufrgs.br/bitstream/10183/211602/2/001115058.pdf.txt1a951dd230d66d1fa2e708ce57b4f555MD52ORIGINAL001115058.pdfTexto completo (inglês)application/pdf1657522http://www.lume.ufrgs.br/bitstream/10183/211602/1/001115058.pdf15548dd6ab27611d0c6da2e548f18284MD5110183/2116022020-07-10 03:42:22.850083oai:www.lume.ufrgs.br:10183/211602Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-07-10T06:42:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
title |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
spellingShingle |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast Ferreira, Tamara Zinn Células satélites de músculo esquelético Fatores de regulação miogênica Macrófagos Colágeno Miopatia Frangos de corte Wooden breast Muscle satellite cell Myogenic stem cell Macrophage Collagen infiltration Broiler chicken |
title_short |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
title_full |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
title_fullStr |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
title_full_unstemmed |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
title_sort |
Characterization of pectoralis major muscle satellite cell population heterogeneity, macrophage density, and collagen infiltration in broiler chickens affected by wooden breast |
author |
Ferreira, Tamara Zinn |
author_facet |
Ferreira, Tamara Zinn Kindlein, Líris Flees, Joshua J. Shortnacy, Lauren K. Vieira, Sérgio Luiz Nascimento, Vladimir Pinheiro do Meloche, Kathryn J. Starkey, Jessica D. |
author_role |
author |
author2 |
Kindlein, Líris Flees, Joshua J. Shortnacy, Lauren K. Vieira, Sérgio Luiz Nascimento, Vladimir Pinheiro do Meloche, Kathryn J. Starkey, Jessica D. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Tamara Zinn Kindlein, Líris Flees, Joshua J. Shortnacy, Lauren K. Vieira, Sérgio Luiz Nascimento, Vladimir Pinheiro do Meloche, Kathryn J. Starkey, Jessica D. |
dc.subject.por.fl_str_mv |
Células satélites de músculo esquelético Fatores de regulação miogênica Macrófagos Colágeno Miopatia Frangos de corte |
topic |
Células satélites de músculo esquelético Fatores de regulação miogênica Macrófagos Colágeno Miopatia Frangos de corte Wooden breast Muscle satellite cell Myogenic stem cell Macrophage Collagen infiltration Broiler chicken |
dc.subject.eng.fl_str_mv |
Wooden breast Muscle satellite cell Myogenic stem cell Macrophage Collagen infiltration Broiler chicken |
description |
Muscle satellite cells (MSCs) are myogenic stem cells that play a critical role in post-hatch skeletal muscle growth and regeneration. Activation of regeneration pathways to repair muscle fiber damage requires both the proliferation and differentiation of different MSC populations as well as the function of resident phagocytic cells such as anti-inflammatory and pro-inflammatory macrophages. The Wooden Breast (WB) phenotype in broiler chickens is characterized by myofiber degeneration and extensive fibrosis. Previous work indicates that the resident MSC populations expressing the myogenic regulatory factors, Myf-5 and Pax7 are larger and more proliferative in broilers severely affected with WB vs. unaffected broilers. To further characterize the cellular and molecular changes occurring in WB-affected muscles, samples from pectoralis major (PM) muscles with varying severity of WB (WB score 0 = normal; 1 = mildly affected; 2 = severely affected) were collected at 25 and 43 days post-hatch (n = 8 per score per age) and processed for cryohistological and protein expression analyses. Collagen per field and densities of macrophages and MyoDC, Myf-5C, and Pax7C MSC populations were quantified on immunofluorescence-stained cryosections. Relative collagen protein expression was quantified by fluorescent Western Blotting. In both 25 and 43-daysold broilers, the proportion of collagen per field (P _ 0.021) and macrophage density (P _ 0.074) were greater in PM exhibiting severe WB compared with normal. At day 43, populations of MyoDC, Myf-5C:MyoDC MSC were larger and relative collagen protein expression was greater in WB-affected vs. unaffected broilers (P _ 0.05). Pax7C MSC relative to total cells was also increased as WB severity increased in 43-days-old broilers (P _ 0.05). Densities of Myf-5C (P = 0.092), MyoDC (P = 0.030), Myf5C:MyoDC (P = 0.046), and Myf-5C:MyoDC:Pax7C (P = 0.048) MSC were greater in WB score 1 birds compared with WB score 0 and 2 birds. Overall, alterations in the resident MSC and macrophage populations and collagen protein content were observed in WBaffected muscle. Further investigation will be required to determine how these changes in cell population kinetics and local autocrine and paracrine signaling are involved in the apparent dysregulation of muscle maintenance in WB-affected broilers. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-07-09T03:41:51Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
format |
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publishedVersion |
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http://hdl.handle.net/10183/211602 |
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1664-042X |
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001115058 |
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http://hdl.handle.net/10183/211602 |
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eng |
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Frontiers in Physiology. Lausanne. Vol. 11 (May 2020), 529, 11 p. |
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openAccess |
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