Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces

• Autor(a) principal: Antonini, Leonardo Marasca
• Data de Publicação: 2018
• Outros Autores: Santos Júnior, Adilar Gonçalves dos, Reilly, Gwendolen, Malfatti, Célia de Fraga
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/187922
• topic: Células-tronco embrionárias; Eletropolimento; Ligas de titânio; Molhabilidade; hESCs-MP cells; Ti6Al4V; Electropolishing; Nanometric and micrometric roughness; Wettability

Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface.