Screening for antimicrobial activity among bacteria isolated from the amazon basin

Detalhes bibliográficos
Autor(a) principal: Motta, Amanda de Souza da
Data de Publicação: 2004
Outros Autores: Olivera, Florencia Cladera, Brandelli, Adriano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/20219
Resumo: Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml-1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels revealed that the molecular weight of the compounds was lower than 14 kDa. Several strains showed antibacterial activity, which in some cases appear to be related to antimicrobial peptides. The potential of these microorganisms to produce useful antimicrobial compounds is great and must be better explored.
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spelling Motta, Amanda de Souza daOlivera, Florencia CladeraBrandelli, Adriano2010-04-16T09:13:35Z20041517-8382http://hdl.handle.net/10183/20219000553653Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml-1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels revealed that the molecular weight of the compounds was lower than 14 kDa. Several strains showed antibacterial activity, which in some cases appear to be related to antimicrobial peptides. The potential of these microorganisms to produce useful antimicrobial compounds is great and must be better explored.application/pdfengBrazilian journal of microbiology. São Paulo. vol. 35, n.4 (2004), p.307-310Atividade antimicrobianaListeria monocytogenesBacteriocinaAntimicrobial activityListeria monocytogenesBacteriocinsAmazon basinScreening for antimicrobial activity among bacteria isolated from the amazon basininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000553653.pdf000553653.pdfTexto completo (inglês)application/pdf108857http://www.lume.ufrgs.br/bitstream/10183/20219/1/000553653.pdf59743cb5f45e2292e9d6b09c9287fb3cMD51TEXT000553653.pdf.txt000553653.pdf.txtExtracted Texttext/plain19997http://www.lume.ufrgs.br/bitstream/10183/20219/2/000553653.pdf.txtb5bb3b1fed3691f9d2749d78d7147cf5MD52THUMBNAIL000553653.pdf.jpg000553653.pdf.jpgGenerated Thumbnailimage/jpeg1633http://www.lume.ufrgs.br/bitstream/10183/20219/3/000553653.pdf.jpgd252e719cf6612c377c0ed3cc471eccdMD5310183/202192018-10-08 09:12:51.188oai:www.lume.ufrgs.br:10183/20219Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-08T12:12:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Screening for antimicrobial activity among bacteria isolated from the amazon basin
title Screening for antimicrobial activity among bacteria isolated from the amazon basin
spellingShingle Screening for antimicrobial activity among bacteria isolated from the amazon basin
Motta, Amanda de Souza da
Atividade antimicrobiana
Listeria monocytogenes
Bacteriocina
Antimicrobial activity
Listeria monocytogenes
Bacteriocins
Amazon basin
title_short Screening for antimicrobial activity among bacteria isolated from the amazon basin
title_full Screening for antimicrobial activity among bacteria isolated from the amazon basin
title_fullStr Screening for antimicrobial activity among bacteria isolated from the amazon basin
title_full_unstemmed Screening for antimicrobial activity among bacteria isolated from the amazon basin
title_sort Screening for antimicrobial activity among bacteria isolated from the amazon basin
author Motta, Amanda de Souza da
author_facet Motta, Amanda de Souza da
Olivera, Florencia Cladera
Brandelli, Adriano
author_role author
author2 Olivera, Florencia Cladera
Brandelli, Adriano
author2_role author
author
dc.contributor.author.fl_str_mv Motta, Amanda de Souza da
Olivera, Florencia Cladera
Brandelli, Adriano
dc.subject.por.fl_str_mv Atividade antimicrobiana
Listeria monocytogenes
Bacteriocina
topic Atividade antimicrobiana
Listeria monocytogenes
Bacteriocina
Antimicrobial activity
Listeria monocytogenes
Bacteriocins
Amazon basin
dc.subject.eng.fl_str_mv Antimicrobial activity
Listeria monocytogenes
Bacteriocins
Amazon basin
description Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml-1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels revealed that the molecular weight of the compounds was lower than 14 kDa. Several strains showed antibacterial activity, which in some cases appear to be related to antimicrobial peptides. The potential of these microorganisms to produce useful antimicrobial compounds is great and must be better explored.
publishDate 2004
dc.date.issued.fl_str_mv 2004
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of microbiology. São Paulo. vol. 35, n.4 (2004), p.307-310
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