Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/221803 |
Resumo: | Background: The described species from the Metarhizium genus are cosmopolitan fungi that infect arthropod hosts. Interestingly, while some species infect a wide range of hosts (host-generalists), other species infect only a few arthropods (host-specialists). This singular evolutionary trait permits unique comparisons to determine how pathogens and virulence determinants emerge. Among the several virulence determinants that have been described, secondary metabolites (SMs) are suggested to play essential roles during fungal infection. Despite progress in the study of pathogen-host relationships, the majority of genes related to SM production in Metarhizium spp. are uncharacterized, and little is known about their genomic organization, expression and regulation. To better understand how infection conditions may affect SM production in Metarhizium anisopliae, we have performed a deep survey and description of SM biosynthetic gene clusters (BGCs) in M. anisopliae, analyzed RNA-seq data from fungi grown on cattle-tick cuticles, evaluated the differential expression of BGCs, and assessed conservation among the Metarhizium genus. Furthermore, our analysis extended to the construction of a phylogeny for the following three BGCs: a tropolone/citrinin-related compound (MaPKS1), a pseurotin-related compound (MaNRPS-PKS2), and a putative helvolic acid (MaTERP1). Results: Among 73 BGCs identified in M. anisopliae, 20 % were up-regulated during initial tick cuticle infection and presumably possess virulence-related roles. These up-regulated BGCs include known clusters, such as destruxin, NG39x and ferricrocin, together with putative helvolic acid and, pseurotin and tropolone/citrinin-related compound clusters as well as uncharacterized clusters. Furthermore, several previously characterized and putative BGCs were silent or down-regulated in initial infection conditions, indicating minor participation over the course of infection. Interestingly, several up-regulated BGCs were not conserved in host-specialist species from the Metarhizium genus, indicating differences in the metabolic strategies employed by generalist and specialist species to overcome and kill their host. These differences in metabolic potential may have been partially shaped by horizontal gene transfer (HGT) events, as our phylogenetic analysis provided evidence that the putative helvolic acid cluster in Metarhizium spp. originated from an HGT event. Conclusions: Several unknown BGCs are described, and aspects of their organization, regulation and origin are discussed, providing further support for the impact of SM on the Metarhizium genus lifestyle and infection process. |
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Oliveira, Nicolau SbarainiGuedes, Rafael Lucas MunizAndreis, Fábio CarrerJunges, AngelaMorais, Guilherme Loss deVainstein, Marilene HenningVasconcelos, Ana Tereza Ribeiro deSchrank, Augusto2021-06-02T04:33:13Z20161471-2164http://hdl.handle.net/10183/221803001015304Background: The described species from the Metarhizium genus are cosmopolitan fungi that infect arthropod hosts. Interestingly, while some species infect a wide range of hosts (host-generalists), other species infect only a few arthropods (host-specialists). This singular evolutionary trait permits unique comparisons to determine how pathogens and virulence determinants emerge. Among the several virulence determinants that have been described, secondary metabolites (SMs) are suggested to play essential roles during fungal infection. Despite progress in the study of pathogen-host relationships, the majority of genes related to SM production in Metarhizium spp. are uncharacterized, and little is known about their genomic organization, expression and regulation. To better understand how infection conditions may affect SM production in Metarhizium anisopliae, we have performed a deep survey and description of SM biosynthetic gene clusters (BGCs) in M. anisopliae, analyzed RNA-seq data from fungi grown on cattle-tick cuticles, evaluated the differential expression of BGCs, and assessed conservation among the Metarhizium genus. Furthermore, our analysis extended to the construction of a phylogeny for the following three BGCs: a tropolone/citrinin-related compound (MaPKS1), a pseurotin-related compound (MaNRPS-PKS2), and a putative helvolic acid (MaTERP1). Results: Among 73 BGCs identified in M. anisopliae, 20 % were up-regulated during initial tick cuticle infection and presumably possess virulence-related roles. These up-regulated BGCs include known clusters, such as destruxin, NG39x and ferricrocin, together with putative helvolic acid and, pseurotin and tropolone/citrinin-related compound clusters as well as uncharacterized clusters. Furthermore, several previously characterized and putative BGCs were silent or down-regulated in initial infection conditions, indicating minor participation over the course of infection. Interestingly, several up-regulated BGCs were not conserved in host-specialist species from the Metarhizium genus, indicating differences in the metabolic strategies employed by generalist and specialist species to overcome and kill their host. These differences in metabolic potential may have been partially shaped by horizontal gene transfer (HGT) events, as our phylogenetic analysis provided evidence that the putative helvolic acid cluster in Metarhizium spp. originated from an HGT event. Conclusions: Several unknown BGCs are described, and aspects of their organization, regulation and origin are discussed, providing further support for the impact of SM on the Metarhizium genus lifestyle and infection process.application/pdfengBMC Genomics. London. Vol. 17, sup. 8(Oct. 2016), e736, p. 400-462Fungo entomopatogenicoMetarhizium anisopliaeTranscriptomaControle biológicoMetarhizium sppSecondary metabolite biosynthetic gene clustersInfection processTranscriptome analysisBiological controlCattle tickSecondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection modelEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001015304.pdf.txt001015304.pdf.txtExtracted Texttext/plain97032http://www.lume.ufrgs.br/bitstream/10183/221803/2/001015304.pdf.txtdab13a4c799e32f2dc98396e43d2ef3cMD52ORIGINAL001015304.pdfTexto completo (inglês)application/pdf2111501http://www.lume.ufrgs.br/bitstream/10183/221803/1/001015304.pdf360b0024c65e9e760bc7b192bc1a092dMD5110183/2218032023-08-12 03:47:14.010862oai:www.lume.ufrgs.br:10183/221803Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-12T06:47:14Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
title |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
spellingShingle |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model Oliveira, Nicolau Sbaraini Fungo entomopatogenico Metarhizium anisopliae Transcriptoma Controle biológico Metarhizium spp Secondary metabolite biosynthetic gene clusters Infection process Transcriptome analysis Biological control Cattle tick |
title_short |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
title_full |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
title_fullStr |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
title_full_unstemmed |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
title_sort |
Secondary metabolite gene clusters in the entomopathogen fungus Metarhizium anisopliae : genome identification and patterns of expression in a cuticle infection model |
author |
Oliveira, Nicolau Sbaraini |
author_facet |
Oliveira, Nicolau Sbaraini Guedes, Rafael Lucas Muniz Andreis, Fábio Carrer Junges, Angela Morais, Guilherme Loss de Vainstein, Marilene Henning Vasconcelos, Ana Tereza Ribeiro de Schrank, Augusto |
author_role |
author |
author2 |
Guedes, Rafael Lucas Muniz Andreis, Fábio Carrer Junges, Angela Morais, Guilherme Loss de Vainstein, Marilene Henning Vasconcelos, Ana Tereza Ribeiro de Schrank, Augusto |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Nicolau Sbaraini Guedes, Rafael Lucas Muniz Andreis, Fábio Carrer Junges, Angela Morais, Guilherme Loss de Vainstein, Marilene Henning Vasconcelos, Ana Tereza Ribeiro de Schrank, Augusto |
dc.subject.por.fl_str_mv |
Fungo entomopatogenico Metarhizium anisopliae Transcriptoma Controle biológico |
topic |
Fungo entomopatogenico Metarhizium anisopliae Transcriptoma Controle biológico Metarhizium spp Secondary metabolite biosynthetic gene clusters Infection process Transcriptome analysis Biological control Cattle tick |
dc.subject.eng.fl_str_mv |
Metarhizium spp Secondary metabolite biosynthetic gene clusters Infection process Transcriptome analysis Biological control Cattle tick |
description |
Background: The described species from the Metarhizium genus are cosmopolitan fungi that infect arthropod hosts. Interestingly, while some species infect a wide range of hosts (host-generalists), other species infect only a few arthropods (host-specialists). This singular evolutionary trait permits unique comparisons to determine how pathogens and virulence determinants emerge. Among the several virulence determinants that have been described, secondary metabolites (SMs) are suggested to play essential roles during fungal infection. Despite progress in the study of pathogen-host relationships, the majority of genes related to SM production in Metarhizium spp. are uncharacterized, and little is known about their genomic organization, expression and regulation. To better understand how infection conditions may affect SM production in Metarhizium anisopliae, we have performed a deep survey and description of SM biosynthetic gene clusters (BGCs) in M. anisopliae, analyzed RNA-seq data from fungi grown on cattle-tick cuticles, evaluated the differential expression of BGCs, and assessed conservation among the Metarhizium genus. Furthermore, our analysis extended to the construction of a phylogeny for the following three BGCs: a tropolone/citrinin-related compound (MaPKS1), a pseurotin-related compound (MaNRPS-PKS2), and a putative helvolic acid (MaTERP1). Results: Among 73 BGCs identified in M. anisopliae, 20 % were up-regulated during initial tick cuticle infection and presumably possess virulence-related roles. These up-regulated BGCs include known clusters, such as destruxin, NG39x and ferricrocin, together with putative helvolic acid and, pseurotin and tropolone/citrinin-related compound clusters as well as uncharacterized clusters. Furthermore, several previously characterized and putative BGCs were silent or down-regulated in initial infection conditions, indicating minor participation over the course of infection. Interestingly, several up-regulated BGCs were not conserved in host-specialist species from the Metarhizium genus, indicating differences in the metabolic strategies employed by generalist and specialist species to overcome and kill their host. These differences in metabolic potential may have been partially shaped by horizontal gene transfer (HGT) events, as our phylogenetic analysis provided evidence that the putative helvolic acid cluster in Metarhizium spp. originated from an HGT event. Conclusions: Several unknown BGCs are described, and aspects of their organization, regulation and origin are discussed, providing further support for the impact of SM on the Metarhizium genus lifestyle and infection process. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2021-06-02T04:33:13Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/221803 |
dc.identifier.issn.pt_BR.fl_str_mv |
1471-2164 |
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001015304 |
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1471-2164 001015304 |
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http://hdl.handle.net/10183/221803 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
BMC Genomics. London. Vol. 17, sup. 8(Oct. 2016), e736, p. 400-462 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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