Cell block technique as an additional tool in the diagnosis of ameloblastoma
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/106722 |
Resumo: | The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/ AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells. |
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Belatto, Maria Fernanda da SilvaRamos, Grasieli de OliveiraMartins, Manoela DominguesGrando, Liliane JaneteRivero, Helena Riet Correa2014-11-12T02:13:34Z20141806-8324http://hdl.handle.net/10183/106722000936420The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/ AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.application/pdfengBrazilian oral research. São Paulo. Vol. 28, no. 1 (Jan. 2014), p. 1-6AmeloblastomaTumores : OdontogenicosBiópsia por agulha finaBiopsyFine-needleCell biologyOdontogenic tumorsAmeloblastomaCell block technique as an additional tool in the diagnosis of ameloblastomainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000936420.pdf000936420.pdfTexto completo (inglês)application/pdf1181788http://www.lume.ufrgs.br/bitstream/10183/106722/1/000936420.pdfe1baf9d9f89b9208ceaef7c421107c2aMD51TEXT000936420.pdf.txt000936420.pdf.txtExtracted Texttext/plain20254http://www.lume.ufrgs.br/bitstream/10183/106722/2/000936420.pdf.txt49c297273aa25cd42b7fe01dbc807c41MD52THUMBNAIL000936420.pdf.jpg000936420.pdf.jpgGenerated Thumbnailimage/jpeg1970http://www.lume.ufrgs.br/bitstream/10183/106722/3/000936420.pdf.jpgfac1f01b6c904a39838c8cbdb193c7daMD5310183/1067222018-10-22 07:47:06.906oai:www.lume.ufrgs.br:10183/106722Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-22T10:47:06Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
title |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
spellingShingle |
Cell block technique as an additional tool in the diagnosis of ameloblastoma Belatto, Maria Fernanda da Silva Ameloblastoma Tumores : Odontogenicos Biópsia por agulha fina Biopsy Fine-needle Cell biology Odontogenic tumors Ameloblastoma |
title_short |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
title_full |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
title_fullStr |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
title_full_unstemmed |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
title_sort |
Cell block technique as an additional tool in the diagnosis of ameloblastoma |
author |
Belatto, Maria Fernanda da Silva |
author_facet |
Belatto, Maria Fernanda da Silva Ramos, Grasieli de Oliveira Martins, Manoela Domingues Grando, Liliane Janete Rivero, Helena Riet Correa |
author_role |
author |
author2 |
Ramos, Grasieli de Oliveira Martins, Manoela Domingues Grando, Liliane Janete Rivero, Helena Riet Correa |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Belatto, Maria Fernanda da Silva Ramos, Grasieli de Oliveira Martins, Manoela Domingues Grando, Liliane Janete Rivero, Helena Riet Correa |
dc.subject.por.fl_str_mv |
Ameloblastoma Tumores : Odontogenicos Biópsia por agulha fina |
topic |
Ameloblastoma Tumores : Odontogenicos Biópsia por agulha fina Biopsy Fine-needle Cell biology Odontogenic tumors Ameloblastoma |
dc.subject.eng.fl_str_mv |
Biopsy Fine-needle Cell biology Odontogenic tumors Ameloblastoma |
description |
The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/ AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-11-12T02:13:34Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.driver.fl_str_mv |
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http://hdl.handle.net/10183/106722 |
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1806-8324 |
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000936420 |
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http://hdl.handle.net/10183/106722 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian oral research. São Paulo. Vol. 28, no. 1 (Jan. 2014), p. 1-6 |
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