Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/215040 |
Resumo: | The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the Brazilian Microbiome Project pipeline for Ion Torrent data with modifications. Statistical analyses were performed in the R software environment. We found that chronic feeding of a commercial NNS-sweetened yogurt to Wistar rats, within the recommended dose range, did not significantly modify gut microbiota composition in comparison to sucrose-sweetened yogurt. Our findings do not support the hypothesis that moderate exposure to NNS is associated with changes in gut microbiota pattern compared to sucrose, at least in this experimental model.1 |
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Lopes, Tiago FalcónFoletto, Kelly CarraroSiebert, MarinaPinto, Denise EntrudoAndrades, Michael EvertonBertoluci, Marcello Casaccia2020-11-14T04:22:42Z20201415-4757http://hdl.handle.net/10183/215040001117871The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the Brazilian Microbiome Project pipeline for Ion Torrent data with modifications. Statistical analyses were performed in the R software environment. We found that chronic feeding of a commercial NNS-sweetened yogurt to Wistar rats, within the recommended dose range, did not significantly modify gut microbiota composition in comparison to sucrose-sweetened yogurt. Our findings do not support the hypothesis that moderate exposure to NNS is associated with changes in gut microbiota pattern compared to sucrose, at least in this experimental model.1application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 43, n.1 (2020), e20190028,6 p.Sequenciamento de nucleotídeos em larga escalaIogurteSacaroseSacarinaMicrobiotaModelos animais6S rDNADeep sequencingSaccharinCyclamateYogurtMetabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001117871.pdf.txt001117871.pdf.txtExtracted Texttext/plain25724http://www.lume.ufrgs.br/bitstream/10183/215040/2/001117871.pdf.txt6e441d79a99a1b7a378580f2ce812fbcMD52ORIGINAL001117871.pdfTexto completo (inglês)application/pdf2119856http://www.lume.ufrgs.br/bitstream/10183/215040/1/001117871.pdfe2c9168baeedb01b69b940360e802234MD5110183/2150402020-11-15 05:11:44.865579oai:www.lume.ufrgs.br:10183/215040Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-11-15T07:11:44Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
title |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
spellingShingle |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats Lopes, Tiago Falcón Sequenciamento de nucleotídeos em larga escala Iogurte Sacarose Sacarina Microbiota Modelos animais 6S rDNA Deep sequencing Saccharin Cyclamate Yogurt |
title_short |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
title_full |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
title_fullStr |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
title_full_unstemmed |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
title_sort |
Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats |
author |
Lopes, Tiago Falcón |
author_facet |
Lopes, Tiago Falcón Foletto, Kelly Carraro Siebert, Marina Pinto, Denise Entrudo Andrades, Michael Everton Bertoluci, Marcello Casaccia |
author_role |
author |
author2 |
Foletto, Kelly Carraro Siebert, Marina Pinto, Denise Entrudo Andrades, Michael Everton Bertoluci, Marcello Casaccia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Tiago Falcón Foletto, Kelly Carraro Siebert, Marina Pinto, Denise Entrudo Andrades, Michael Everton Bertoluci, Marcello Casaccia |
dc.subject.por.fl_str_mv |
Sequenciamento de nucleotídeos em larga escala Iogurte Sacarose Sacarina Microbiota Modelos animais |
topic |
Sequenciamento de nucleotídeos em larga escala Iogurte Sacarose Sacarina Microbiota Modelos animais 6S rDNA Deep sequencing Saccharin Cyclamate Yogurt |
dc.subject.eng.fl_str_mv |
6S rDNA Deep sequencing Saccharin Cyclamate Yogurt |
description |
The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the Brazilian Microbiome Project pipeline for Ion Torrent data with modifications. Statistical analyses were performed in the R software environment. We found that chronic feeding of a commercial NNS-sweetened yogurt to Wistar rats, within the recommended dose range, did not significantly modify gut microbiota composition in comparison to sucrose-sweetened yogurt. Our findings do not support the hypothesis that moderate exposure to NNS is associated with changes in gut microbiota pattern compared to sucrose, at least in this experimental model.1 |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-11-14T04:22:42Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/215040 |
dc.identifier.issn.pt_BR.fl_str_mv |
1415-4757 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001117871 |
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1415-4757 001117871 |
url |
http://hdl.handle.net/10183/215040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto. Vol. 43, n.1 (2020), e20190028,6 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
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