Alkamides: a new class of plant growth regulators linked to humic acid bioactivity

Detalhes bibliográficos
Autor(a) principal: Zandonadi, Daniel Basílio
Data de Publicação: 2019
Outros Autores: Matos, Carlos Roberto Ribeiro, Castro, Rosane Nora, Spaccini, Riccardo, Olivares, Fábio Lopes, Canellas, Luciano Pasqualoto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRJ
Texto Completo: http://hdl.handle.net/11422/12721
Resumo: O uso de substâncias húmicas como bioestimulantes vegetais tem atraído cada vez mais agricultores. A capacidade dessas substâncias de melhorar o crescimento radicular alterando a arquitetura radicular está frequentemente ligada às suas atividades hormonais, como efeitos da auxina e produção de óxido nítrico. A humeômica acessa os constituintes moleculares das substâncias húmicas, revelando a importância dos componentes alquil devido às suas conformações e atividades químicas. Aqui, descrevemos as alquilamidas presentes nos ácidos húmicos e comparamos sua bioatividade usando a atividade da membrana plasmática H + -ATPase como marcadora.
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spelling Zandonadi, Daniel BasílioMatos, Carlos Roberto RibeiroCastro, Rosane NoraSpaccini, RiccardoOlivares, Fábio LopesCanellas, Luciano Pasqualoto2020-07-14T13:02:26Z2023-11-30T03:00:34Z2019-12-05ZANDONADI, Daniel Basílio et al. Alkamides: a new class of plant growth regulators linked to humic acid bioactivity. Chemical and Biological Technologies in Agriculture, 6, 23, 2019. Disponível em: https://doi.org/10.1186/s40538-019-0161-42196-5641http://hdl.handle.net/11422/1272110.1186/s40538-019-0161-4O uso de substâncias húmicas como bioestimulantes vegetais tem atraído cada vez mais agricultores. A capacidade dessas substâncias de melhorar o crescimento radicular alterando a arquitetura radicular está frequentemente ligada às suas atividades hormonais, como efeitos da auxina e produção de óxido nítrico. A humeômica acessa os constituintes moleculares das substâncias húmicas, revelando a importância dos componentes alquil devido às suas conformações e atividades químicas. Aqui, descrevemos as alquilamidas presentes nos ácidos húmicos e comparamos sua bioatividade usando a atividade da membrana plasmática H + -ATPase como marcadora.The use of humic substances as plant biostimulants has been increasingly attracting farmers and stunning researchers. The ability of these substances to enhance root growth by changing root architecture is often linked to their hormonal activities, such as auxin effects and nitric oxide production. Humeomics accesses the molecular constituents of humic substances, revealing the importance of alkyl components because of their conformations and chemical activities. Here, we describe the alkamides present in humic acids and compare their bioactivities using plasma membrane H+-ATPase activity level as a biochemical marker. Humic acids isolated from vermicompost were analyzed using 13C and 15N nuclear magnetic resonance spectroscopy. The unbound fraction was extracted with ethyl acetate and submitted to gas chromatography coupled to mass spectrometry to detect the presence of N-isopropyldecanamide. We synthesized N-isopropyldecanamide and treated maize seedlings for 7 and 15 days with different concentrations. The root growth and plasma membrane H+-ATPase activity were monitored. Nitric oxide accumulation in the lateral roots was imaged using 4,5-diaminofluorescein diacetate. The results were compared with those obtained for seedlings treated with humic acids isolated from vermicompost. The amide functional group produced the only nitrogen signal in the 15N humic acid resonance spectrum and similar alkamide moieties were found in the unbound humic extract through comparisons using gas chromatography coupled to mass spectrometry. The synthesis of N-isopropyldecanamide had few steps and produced a high yield (86%). The effects of N-isopropyldecanamide on root growth were concentration dependent. High concentrations (10−4 M) enhanced root growth after 15 day of diminishing shoot biomass. However, low concentrations (10−8 M and 10−6 M) promoted root growth at 7 and 15 days, similar to the humic acid-induced plasma membrane H+-ATPase activity. Both N-isopropyldecanamide and humic acids enhanced nitric oxide accumulation during lateral root emergence. Conclusion We described for the first time the effects of N-isopropyldecanamide on the plasma membrane H+-ATPase activity in maize seedling roots and compared its effects with those caused by humic acids. N-Isopropyldecanamide was detected in the unbound fraction of the humic supramolecular assembly, indicating that the putative hormone-like effects of these substances result also from the presence of this new class of plant regulators, in addition to other molecules.Submitted by DANIEL ZANDONADI (danielzandonadi@macae.ufrj.br) on 2020-07-10T13:14:49Z No. of bitstreams: 1 document(9).pdf: 1695625 bytes, checksum: 115f9ed9b1eba94aa7de3755cf1c9d89 (MD5)Approved for entry into archive by Maria Luiza Jardim (luiza@sibi.ufrj.br) on 2020-07-14T13:02:26Z (GMT) No. of bitstreams: 1 document(9).pdf: 1695625 bytes, checksum: 115f9ed9b1eba94aa7de3755cf1c9d89 (MD5)Made available in DSpace on 2020-07-14T13:02:26Z (GMT). No. of bitstreams: 1 document(9).pdf: 1695625 bytes, checksum: 115f9ed9b1eba94aa7de3755cf1c9d89 (MD5) Previous issue date: 2019-12-05engSpringerOpenAlemanhaInstituto de Biodiversidade e SustentabilidadeChemical and Biological Technologies in AgricultureAn error occurred getting the license - uri.info:eu-repo/semantics/openAccessCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLOLipídiosReguladores de crescimento de plantaSubstâncias húmicasAffininsSmall lipidsHormone-like effectsPlant growth regulatorsHumic substancesAlkamides: a new class of plant growth regulators linked to humic acid bioactivityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6abertoreponame:Repositório Institucional da UFRJinstname:Universidade Federal do Rio de Janeiro (UFRJ)instacron:UFRJORIGINALdocument(9).pdfdocument(9).pdfapplication/pdf1695625http://pantheon.ufrj.br:80/bitstream/11422/12721/1/document%289%29.pdf115f9ed9b1eba94aa7de3755cf1c9d89MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81853http://pantheon.ufrj.br:80/bitstream/11422/12721/2/license.txtdd32849f2bfb22da963c3aac6e26e255MD5211422/127212023-11-30 00:00:34.107oai:pantheon.ufrj.br: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Repositório de PublicaçõesPUBhttp://www.pantheon.ufrj.br/oai/requestopendoar:2023-11-30T03:00:34Repositório Institucional da UFRJ - Universidade Federal do Rio de Janeiro (UFRJ)false
dc.title.pt_BR.fl_str_mv Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
title Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
spellingShingle Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
Zandonadi, Daniel Basílio
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLO
Lipídios
Reguladores de crescimento de planta
Substâncias húmicas
Affinins
Small lipids
Hormone-like effects
Plant growth regulators
Humic substances
title_short Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
title_full Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
title_fullStr Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
title_full_unstemmed Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
title_sort Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
author Zandonadi, Daniel Basílio
author_facet Zandonadi, Daniel Basílio
Matos, Carlos Roberto Ribeiro
Castro, Rosane Nora
Spaccini, Riccardo
Olivares, Fábio Lopes
Canellas, Luciano Pasqualoto
author_role author
author2 Matos, Carlos Roberto Ribeiro
Castro, Rosane Nora
Spaccini, Riccardo
Olivares, Fábio Lopes
Canellas, Luciano Pasqualoto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Zandonadi, Daniel Basílio
Matos, Carlos Roberto Ribeiro
Castro, Rosane Nora
Spaccini, Riccardo
Olivares, Fábio Lopes
Canellas, Luciano Pasqualoto
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLO
topic CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLO
Lipídios
Reguladores de crescimento de planta
Substâncias húmicas
Affinins
Small lipids
Hormone-like effects
Plant growth regulators
Humic substances
dc.subject.por.fl_str_mv Lipídios
Reguladores de crescimento de planta
Substâncias húmicas
dc.subject.eng.fl_str_mv Affinins
Small lipids
Hormone-like effects
Plant growth regulators
Humic substances
description O uso de substâncias húmicas como bioestimulantes vegetais tem atraído cada vez mais agricultores. A capacidade dessas substâncias de melhorar o crescimento radicular alterando a arquitetura radicular está frequentemente ligada às suas atividades hormonais, como efeitos da auxina e produção de óxido nítrico. A humeômica acessa os constituintes moleculares das substâncias húmicas, revelando a importância dos componentes alquil devido às suas conformações e atividades químicas. Aqui, descrevemos as alquilamidas presentes nos ácidos húmicos e comparamos sua bioatividade usando a atividade da membrana plasmática H + -ATPase como marcadora.
publishDate 2019
dc.date.issued.fl_str_mv 2019-12-05
dc.date.accessioned.fl_str_mv 2020-07-14T13:02:26Z
dc.date.available.fl_str_mv 2023-11-30T03:00:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ZANDONADI, Daniel Basílio et al. Alkamides: a new class of plant growth regulators linked to humic acid bioactivity. Chemical and Biological Technologies in Agriculture, 6, 23, 2019. Disponível em: https://doi.org/10.1186/s40538-019-0161-4
dc.identifier.uri.fl_str_mv http://hdl.handle.net/11422/12721
dc.identifier.issn.pt_BR.fl_str_mv 2196-5641
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s40538-019-0161-4
identifier_str_mv ZANDONADI, Daniel Basílio et al. Alkamides: a new class of plant growth regulators linked to humic acid bioactivity. Chemical and Biological Technologies in Agriculture, 6, 23, 2019. Disponível em: https://doi.org/10.1186/s40538-019-0161-4
2196-5641
10.1186/s40538-019-0161-4
url http://hdl.handle.net/11422/12721
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Chemical and Biological Technologies in Agriculture
dc.rights.driver.fl_str_mv An error occurred getting the license - uri.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv An error occurred getting the license - uri.
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv SpringerOpen
dc.publisher.country.fl_str_mv Alemanha
dc.publisher.department.fl_str_mv Instituto de Biodiversidade e Sustentabilidade
publisher.none.fl_str_mv SpringerOpen
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRJ
instname:Universidade Federal do Rio de Janeiro (UFRJ)
instacron:UFRJ
instname_str Universidade Federal do Rio de Janeiro (UFRJ)
instacron_str UFRJ
institution UFRJ
reponame_str Repositório Institucional da UFRJ
collection Repositório Institucional da UFRJ
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