Alkamides: a new class of plant growth regulators linked to humic acid bioactivity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRJ |
Texto Completo: | http://hdl.handle.net/11422/12721 |
Resumo: | O uso de substâncias húmicas como bioestimulantes vegetais tem atraído cada vez mais agricultores. A capacidade dessas substâncias de melhorar o crescimento radicular alterando a arquitetura radicular está frequentemente ligada às suas atividades hormonais, como efeitos da auxina e produção de óxido nítrico. A humeômica acessa os constituintes moleculares das substâncias húmicas, revelando a importância dos componentes alquil devido às suas conformações e atividades químicas. Aqui, descrevemos as alquilamidas presentes nos ácidos húmicos e comparamos sua bioatividade usando a atividade da membrana plasmática H + -ATPase como marcadora. |
id |
UFRJ_643025853f62dea8b75c70ce89110b8e |
---|---|
oai_identifier_str |
oai:pantheon.ufrj.br:11422/12721 |
network_acronym_str |
UFRJ |
network_name_str |
Repositório Institucional da UFRJ |
repository_id_str |
|
spelling |
Zandonadi, Daniel BasílioMatos, Carlos Roberto RibeiroCastro, Rosane NoraSpaccini, RiccardoOlivares, Fábio LopesCanellas, Luciano Pasqualoto2020-07-14T13:02:26Z2023-11-30T03:00:34Z2019-12-05ZANDONADI, Daniel Basílio et al. Alkamides: a new class of plant growth regulators linked to humic acid bioactivity. Chemical and Biological Technologies in Agriculture, 6, 23, 2019. Disponível em: https://doi.org/10.1186/s40538-019-0161-42196-5641http://hdl.handle.net/11422/1272110.1186/s40538-019-0161-4O uso de substâncias húmicas como bioestimulantes vegetais tem atraído cada vez mais agricultores. A capacidade dessas substâncias de melhorar o crescimento radicular alterando a arquitetura radicular está frequentemente ligada às suas atividades hormonais, como efeitos da auxina e produção de óxido nítrico. A humeômica acessa os constituintes moleculares das substâncias húmicas, revelando a importância dos componentes alquil devido às suas conformações e atividades químicas. Aqui, descrevemos as alquilamidas presentes nos ácidos húmicos e comparamos sua bioatividade usando a atividade da membrana plasmática H + -ATPase como marcadora.The use of humic substances as plant biostimulants has been increasingly attracting farmers and stunning researchers. The ability of these substances to enhance root growth by changing root architecture is often linked to their hormonal activities, such as auxin effects and nitric oxide production. Humeomics accesses the molecular constituents of humic substances, revealing the importance of alkyl components because of their conformations and chemical activities. Here, we describe the alkamides present in humic acids and compare their bioactivities using plasma membrane H+-ATPase activity level as a biochemical marker. Humic acids isolated from vermicompost were analyzed using 13C and 15N nuclear magnetic resonance spectroscopy. The unbound fraction was extracted with ethyl acetate and submitted to gas chromatography coupled to mass spectrometry to detect the presence of N-isopropyldecanamide. We synthesized N-isopropyldecanamide and treated maize seedlings for 7 and 15 days with different concentrations. The root growth and plasma membrane H+-ATPase activity were monitored. Nitric oxide accumulation in the lateral roots was imaged using 4,5-diaminofluorescein diacetate. The results were compared with those obtained for seedlings treated with humic acids isolated from vermicompost. The amide functional group produced the only nitrogen signal in the 15N humic acid resonance spectrum and similar alkamide moieties were found in the unbound humic extract through comparisons using gas chromatography coupled to mass spectrometry. The synthesis of N-isopropyldecanamide had few steps and produced a high yield (86%). The effects of N-isopropyldecanamide on root growth were concentration dependent. High concentrations (10−4 M) enhanced root growth after 15 day of diminishing shoot biomass. However, low concentrations (10−8 M and 10−6 M) promoted root growth at 7 and 15 days, similar to the humic acid-induced plasma membrane H+-ATPase activity. Both N-isopropyldecanamide and humic acids enhanced nitric oxide accumulation during lateral root emergence. Conclusion We described for the first time the effects of N-isopropyldecanamide on the plasma membrane H+-ATPase activity in maize seedling roots and compared its effects with those caused by humic acids. N-Isopropyldecanamide was detected in the unbound fraction of the humic supramolecular assembly, indicating that the putative hormone-like effects of these substances result also from the presence of this new class of plant regulators, in addition to other molecules.Submitted by DANIEL ZANDONADI (danielzandonadi@macae.ufrj.br) on 2020-07-10T13:14:49Z No. of bitstreams: 1 document(9).pdf: 1695625 bytes, checksum: 115f9ed9b1eba94aa7de3755cf1c9d89 (MD5)Approved for entry into archive by Maria Luiza Jardim (luiza@sibi.ufrj.br) on 2020-07-14T13:02:26Z (GMT) No. of bitstreams: 1 document(9).pdf: 1695625 bytes, checksum: 115f9ed9b1eba94aa7de3755cf1c9d89 (MD5)Made available in DSpace on 2020-07-14T13:02:26Z (GMT). No. of bitstreams: 1 document(9).pdf: 1695625 bytes, checksum: 115f9ed9b1eba94aa7de3755cf1c9d89 (MD5) Previous issue date: 2019-12-05engSpringerOpenAlemanhaInstituto de Biodiversidade e SustentabilidadeChemical and Biological Technologies in AgricultureAn error occurred getting the license - uri.info:eu-repo/semantics/openAccessCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLOLipídiosReguladores de crescimento de plantaSubstâncias húmicasAffininsSmall lipidsHormone-like effectsPlant growth regulatorsHumic substancesAlkamides: a new class of plant growth regulators linked to humic acid bioactivityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6abertoreponame:Repositório Institucional da UFRJinstname:Universidade Federal do Rio de Janeiro (UFRJ)instacron:UFRJORIGINALdocument(9).pdfdocument(9).pdfapplication/pdf1695625http://pantheon.ufrj.br:80/bitstream/11422/12721/1/document%289%29.pdf115f9ed9b1eba94aa7de3755cf1c9d89MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81853http://pantheon.ufrj.br:80/bitstream/11422/12721/2/license.txtdd32849f2bfb22da963c3aac6e26e255MD5211422/127212023-11-30 00:00:34.107oai:pantheon.ufrj.br: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Repositório de PublicaçõesPUBhttp://www.pantheon.ufrj.br/oai/requestopendoar:2023-11-30T03:00:34Repositório Institucional da UFRJ - Universidade Federal do Rio de Janeiro (UFRJ)false |
dc.title.pt_BR.fl_str_mv |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
title |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
spellingShingle |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity Zandonadi, Daniel Basílio CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLO Lipídios Reguladores de crescimento de planta Substâncias húmicas Affinins Small lipids Hormone-like effects Plant growth regulators Humic substances |
title_short |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
title_full |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
title_fullStr |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
title_full_unstemmed |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
title_sort |
Alkamides: a new class of plant growth regulators linked to humic acid bioactivity |
author |
Zandonadi, Daniel Basílio |
author_facet |
Zandonadi, Daniel Basílio Matos, Carlos Roberto Ribeiro Castro, Rosane Nora Spaccini, Riccardo Olivares, Fábio Lopes Canellas, Luciano Pasqualoto |
author_role |
author |
author2 |
Matos, Carlos Roberto Ribeiro Castro, Rosane Nora Spaccini, Riccardo Olivares, Fábio Lopes Canellas, Luciano Pasqualoto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Zandonadi, Daniel Basílio Matos, Carlos Roberto Ribeiro Castro, Rosane Nora Spaccini, Riccardo Olivares, Fábio Lopes Canellas, Luciano Pasqualoto |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLO |
topic |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::QUIMICA DO SOLO Lipídios Reguladores de crescimento de planta Substâncias húmicas Affinins Small lipids Hormone-like effects Plant growth regulators Humic substances |
dc.subject.por.fl_str_mv |
Lipídios Reguladores de crescimento de planta Substâncias húmicas |
dc.subject.eng.fl_str_mv |
Affinins Small lipids Hormone-like effects Plant growth regulators Humic substances |
description |
O uso de substâncias húmicas como bioestimulantes vegetais tem atraído cada vez mais agricultores. A capacidade dessas substâncias de melhorar o crescimento radicular alterando a arquitetura radicular está frequentemente ligada às suas atividades hormonais, como efeitos da auxina e produção de óxido nítrico. A humeômica acessa os constituintes moleculares das substâncias húmicas, revelando a importância dos componentes alquil devido às suas conformações e atividades químicas. Aqui, descrevemos as alquilamidas presentes nos ácidos húmicos e comparamos sua bioatividade usando a atividade da membrana plasmática H + -ATPase como marcadora. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-12-05 |
dc.date.accessioned.fl_str_mv |
2020-07-14T13:02:26Z |
dc.date.available.fl_str_mv |
2023-11-30T03:00:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ZANDONADI, Daniel Basílio et al. Alkamides: a new class of plant growth regulators linked to humic acid bioactivity. Chemical and Biological Technologies in Agriculture, 6, 23, 2019. Disponível em: https://doi.org/10.1186/s40538-019-0161-4 |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11422/12721 |
dc.identifier.issn.pt_BR.fl_str_mv |
2196-5641 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/s40538-019-0161-4 |
identifier_str_mv |
ZANDONADI, Daniel Basílio et al. Alkamides: a new class of plant growth regulators linked to humic acid bioactivity. Chemical and Biological Technologies in Agriculture, 6, 23, 2019. Disponível em: https://doi.org/10.1186/s40538-019-0161-4 2196-5641 10.1186/s40538-019-0161-4 |
url |
http://hdl.handle.net/11422/12721 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Chemical and Biological Technologies in Agriculture |
dc.rights.driver.fl_str_mv |
An error occurred getting the license - uri. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
An error occurred getting the license - uri. |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
SpringerOpen |
dc.publisher.country.fl_str_mv |
Alemanha |
dc.publisher.department.fl_str_mv |
Instituto de Biodiversidade e Sustentabilidade |
publisher.none.fl_str_mv |
SpringerOpen |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRJ instname:Universidade Federal do Rio de Janeiro (UFRJ) instacron:UFRJ |
instname_str |
Universidade Federal do Rio de Janeiro (UFRJ) |
instacron_str |
UFRJ |
institution |
UFRJ |
reponame_str |
Repositório Institucional da UFRJ |
collection |
Repositório Institucional da UFRJ |
bitstream.url.fl_str_mv |
http://pantheon.ufrj.br:80/bitstream/11422/12721/1/document%289%29.pdf http://pantheon.ufrj.br:80/bitstream/11422/12721/2/license.txt |
bitstream.checksum.fl_str_mv |
115f9ed9b1eba94aa7de3755cf1c9d89 dd32849f2bfb22da963c3aac6e26e255 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRJ - Universidade Federal do Rio de Janeiro (UFRJ) |
repository.mail.fl_str_mv |
|
_version_ |
1784097185502068736 |