Monitoring cell-cell contacts in vivo in transgenic animals

Detalhes bibliográficos
Autor(a) principal: Huang, Ting-Hao
Data de Publicação: 2016
Outros Autores: Velho, Tarciso André Ferreira, Lois, Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/23071
Resumo: We used a synthetic genetic system based on ligand-induced intramembrane proteolysis to monitor cell-cell contacts in animals. Upon ligand-receptor interaction in sites of cell-cell contact, the transmembrane domain of an engineered receptor is cleaved by intramembrane proteolysis and releases a protein fragment that regulates transcription in the interacting partners. We demonstrate that the system can be used to regulate gene expression between interacting cells, both in vitro and in vivo, in transgenic Drosophila We show that the system allows for detection of interactions between neurons and glia in the Drosophila nervous system. In addition, we observed that when the ligand is expressed in subsets of neurons with a restricted localization in the brain it leads to activation of transcription in a selected set of glial cells that interact with those neurons. This system will be useful to monitor cell-cell interactions in animals, and can be used to genetically manipulate cells that interact with one another.
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spelling Huang, Ting-HaoVelho, Tarciso André FerreiraLois, Carlos2017-05-25T11:53:25Z2017-05-25T11:53:25Z2016https://repositorio.ufrn.br/jspui/handle/123456789/2307110.1242/dev.142406engInteracting cellsDevelopmentAdhesionMorphogenesisCell communicationMonitoring cell-cell contacts in vivo in transgenic animalsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleWe used a synthetic genetic system based on ligand-induced intramembrane proteolysis to monitor cell-cell contacts in animals. Upon ligand-receptor interaction in sites of cell-cell contact, the transmembrane domain of an engineered receptor is cleaved by intramembrane proteolysis and releases a protein fragment that regulates transcription in the interacting partners. We demonstrate that the system can be used to regulate gene expression between interacting cells, both in vitro and in vivo, in transgenic Drosophila We show that the system allows for detection of interactions between neurons and glia in the Drosophila nervous system. In addition, we observed that when the ligand is expressed in subsets of neurons with a restricted localization in the brain it leads to activation of transcription in a selected set of glial cells that interact with those neurons. 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dc.title.pt_BR.fl_str_mv Monitoring cell-cell contacts in vivo in transgenic animals
title Monitoring cell-cell contacts in vivo in transgenic animals
spellingShingle Monitoring cell-cell contacts in vivo in transgenic animals
Huang, Ting-Hao
Interacting cells
Development
Adhesion
Morphogenesis
Cell communication
title_short Monitoring cell-cell contacts in vivo in transgenic animals
title_full Monitoring cell-cell contacts in vivo in transgenic animals
title_fullStr Monitoring cell-cell contacts in vivo in transgenic animals
title_full_unstemmed Monitoring cell-cell contacts in vivo in transgenic animals
title_sort Monitoring cell-cell contacts in vivo in transgenic animals
author Huang, Ting-Hao
author_facet Huang, Ting-Hao
Velho, Tarciso André Ferreira
Lois, Carlos
author_role author
author2 Velho, Tarciso André Ferreira
Lois, Carlos
author2_role author
author
dc.contributor.author.fl_str_mv Huang, Ting-Hao
Velho, Tarciso André Ferreira
Lois, Carlos
dc.subject.por.fl_str_mv Interacting cells
Development
Adhesion
Morphogenesis
Cell communication
topic Interacting cells
Development
Adhesion
Morphogenesis
Cell communication
description We used a synthetic genetic system based on ligand-induced intramembrane proteolysis to monitor cell-cell contacts in animals. Upon ligand-receptor interaction in sites of cell-cell contact, the transmembrane domain of an engineered receptor is cleaved by intramembrane proteolysis and releases a protein fragment that regulates transcription in the interacting partners. We demonstrate that the system can be used to regulate gene expression between interacting cells, both in vitro and in vivo, in transgenic Drosophila We show that the system allows for detection of interactions between neurons and glia in the Drosophila nervous system. In addition, we observed that when the ligand is expressed in subsets of neurons with a restricted localization in the brain it leads to activation of transcription in a selected set of glial cells that interact with those neurons. This system will be useful to monitor cell-cell interactions in animals, and can be used to genetically manipulate cells that interact with one another.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-05-25T11:53:25Z
dc.date.available.fl_str_mv 2017-05-25T11:53:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/23071
dc.identifier.doi.none.fl_str_mv 10.1242/dev.142406
url https://repositorio.ufrn.br/jspui/handle/123456789/23071
identifier_str_mv 10.1242/dev.142406
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
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