Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds

Detalhes bibliográficos
Autor(a) principal: Morais, Ana Heloneida Araújo
Data de Publicação: 2020
Outros Autores: Costa, Rafael Oliveira de Araújo, Matias, Lídia Leonize Rodrigues, Passos, Thaís Souza, Queiroz, Jaluza Luana Carvalho de, Carvalho, Fabiana Maria Coimbra de, Maciel, Bruna Leal Lima, Uchôa, Adriana Ferreira, Amado, Isabel Rodríguez, Gonçalves, Catarina, Pastrana, Lorenzo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/54627
https://doi.org/10.1016/j.fufo.2020.100001
Resumo: The trypsin inhibitor isolated from tamarind seeds(TTI) presentssatietogenic and anti-inflammatory effects. In the present study, TTI encapsulation by nanoprecipitation in purified chitosan and whey protein isolate (ECW) was performed to maintain TTI’s integrity and protection through the gastrointestinal tract. The interaction between TTI and the encapsulating agents was determined by filtration monitoring the antitrypsin activity. Cytotoxicity was evaluated at different concentrations (0.5, 2.5 and 5.0 mg/mL) in Caco-2 and CCD-18Co cells. Subacute blood toxicity was evaluated in Wistar rats (12.5 mg/kg) for ten days. The particles presented a smooth surface, with 118.00 (17.27) nm of diameter, 0.37 (0.02) of polydispersity index, -38.26 (0.15) mV (neutral pH) of sur face charge, and 95.31 (0.31)% of incorporation efficiency. At neutral pH, there was a strong interaction of the encapsulating agents and TTI, and a gradual release of TTI at pH 3.0. Cell viability (> 70%) evaluation, in vivo biochemical and hepatic parameters of Wistar rats showed the absence of toxic effects of the nanoparticles with TTI. The study showed that TTI nanoparticles are potentially safe and represent a promising formulation for the clinical application of TTI and may become an option as a novel ingredient in food
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spelling Morais, Ana Heloneida AraújoCosta, Rafael Oliveira de AraújoMatias, Lídia Leonize RodriguesPassos, Thaís SouzaQueiroz, Jaluza Luana Carvalho deCarvalho, Fabiana Maria Coimbra deMaciel, Bruna Leal LimaUchôa, Adriana FerreiraAmado, Isabel RodríguezGonçalves, CatarinaPastrana, Lorenzohttps://orcid.org/0000-0002-6460-911X2023-08-28T22:26:29Z2023-08-28T22:26:29Z2020COSTA, Rafael Oliveira de Araújo et al. Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds. Future Foods, v. 1, p. 100001, 2020. Disponível em: https://www.sciencedirect.com/science/article/pii/S2666833520300010?via%3Dihub. Acesso em: 5 jul.2023.https://repositorio.ufrn.br/handle/123456789/54627https://doi.org/10.1016/j.fufo.2020.100001ElsevierAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessTamarindus indica L.cytotoxicityCaco-2CCD-18CotoxicitynanoencapsulationbiopolymerSafety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seedsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe trypsin inhibitor isolated from tamarind seeds(TTI) presentssatietogenic and anti-inflammatory effects. In the present study, TTI encapsulation by nanoprecipitation in purified chitosan and whey protein isolate (ECW) was performed to maintain TTI’s integrity and protection through the gastrointestinal tract. The interaction between TTI and the encapsulating agents was determined by filtration monitoring the antitrypsin activity. Cytotoxicity was evaluated at different concentrations (0.5, 2.5 and 5.0 mg/mL) in Caco-2 and CCD-18Co cells. Subacute blood toxicity was evaluated in Wistar rats (12.5 mg/kg) for ten days. The particles presented a smooth surface, with 118.00 (17.27) nm of diameter, 0.37 (0.02) of polydispersity index, -38.26 (0.15) mV (neutral pH) of sur face charge, and 95.31 (0.31)% of incorporation efficiency. At neutral pH, there was a strong interaction of the encapsulating agents and TTI, and a gradual release of TTI at pH 3.0. Cell viability (> 70%) evaluation, in vivo biochemical and hepatic parameters of Wistar rats showed the absence of toxic effects of the nanoparticles with TTI. The study showed that TTI nanoparticles are potentially safe and represent a promising formulation for the clinical application of TTI and may become an option as a novel ingredient in foodengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALSafetyandpotentialfunctionalityofnanoparticles_Costa_2020.pdfSafetyandpotentialfunctionalityofnanoparticles_Costa_2020.pdfapplication/pdf1646746https://repositorio.ufrn.br/bitstream/123456789/54627/1/Safetyandpotentialfunctionalityofnanoparticles_Costa_2020.pdf5919f5b40a65841e087a30818dd3cb7eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufrn.br/bitstream/123456789/54627/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/54627/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/546272023-08-28 19:26:29.732oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-08-28T22:26:29Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
title Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
spellingShingle Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
Morais, Ana Heloneida Araújo
Tamarindus indica L.
cytotoxicity
Caco-2
CCD-18Co
toxicity
nanoencapsulation
biopolymer
title_short Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
title_full Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
title_fullStr Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
title_full_unstemmed Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
title_sort Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds
author Morais, Ana Heloneida Araújo
author_facet Morais, Ana Heloneida Araújo
Costa, Rafael Oliveira de Araújo
Matias, Lídia Leonize Rodrigues
Passos, Thaís Souza
Queiroz, Jaluza Luana Carvalho de
Carvalho, Fabiana Maria Coimbra de
Maciel, Bruna Leal Lima
Uchôa, Adriana Ferreira
Amado, Isabel Rodríguez
Gonçalves, Catarina
Pastrana, Lorenzo
author_role author
author2 Costa, Rafael Oliveira de Araújo
Matias, Lídia Leonize Rodrigues
Passos, Thaís Souza
Queiroz, Jaluza Luana Carvalho de
Carvalho, Fabiana Maria Coimbra de
Maciel, Bruna Leal Lima
Uchôa, Adriana Ferreira
Amado, Isabel Rodríguez
Gonçalves, Catarina
Pastrana, Lorenzo
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.authorID.pt_BR.fl_str_mv https://orcid.org/0000-0002-6460-911X
dc.contributor.author.fl_str_mv Morais, Ana Heloneida Araújo
Costa, Rafael Oliveira de Araújo
Matias, Lídia Leonize Rodrigues
Passos, Thaís Souza
Queiroz, Jaluza Luana Carvalho de
Carvalho, Fabiana Maria Coimbra de
Maciel, Bruna Leal Lima
Uchôa, Adriana Ferreira
Amado, Isabel Rodríguez
Gonçalves, Catarina
Pastrana, Lorenzo
dc.subject.por.fl_str_mv Tamarindus indica L.
cytotoxicity
Caco-2
CCD-18Co
toxicity
nanoencapsulation
biopolymer
topic Tamarindus indica L.
cytotoxicity
Caco-2
CCD-18Co
toxicity
nanoencapsulation
biopolymer
description The trypsin inhibitor isolated from tamarind seeds(TTI) presentssatietogenic and anti-inflammatory effects. In the present study, TTI encapsulation by nanoprecipitation in purified chitosan and whey protein isolate (ECW) was performed to maintain TTI’s integrity and protection through the gastrointestinal tract. The interaction between TTI and the encapsulating agents was determined by filtration monitoring the antitrypsin activity. Cytotoxicity was evaluated at different concentrations (0.5, 2.5 and 5.0 mg/mL) in Caco-2 and CCD-18Co cells. Subacute blood toxicity was evaluated in Wistar rats (12.5 mg/kg) for ten days. The particles presented a smooth surface, with 118.00 (17.27) nm of diameter, 0.37 (0.02) of polydispersity index, -38.26 (0.15) mV (neutral pH) of sur face charge, and 95.31 (0.31)% of incorporation efficiency. At neutral pH, there was a strong interaction of the encapsulating agents and TTI, and a gradual release of TTI at pH 3.0. Cell viability (> 70%) evaluation, in vivo biochemical and hepatic parameters of Wistar rats showed the absence of toxic effects of the nanoparticles with TTI. The study showed that TTI nanoparticles are potentially safe and represent a promising formulation for the clinical application of TTI and may become an option as a novel ingredient in food
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-08-28T22:26:29Z
dc.date.available.fl_str_mv 2023-08-28T22:26:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Rafael Oliveira de Araújo et al. Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds. Future Foods, v. 1, p. 100001, 2020. Disponível em: https://www.sciencedirect.com/science/article/pii/S2666833520300010?via%3Dihub. Acesso em: 5 jul.2023.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/54627
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.fufo.2020.100001
identifier_str_mv COSTA, Rafael Oliveira de Araújo et al. Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds. Future Foods, v. 1, p. 100001, 2020. Disponível em: https://www.sciencedirect.com/science/article/pii/S2666833520300010?via%3Dihub. Acesso em: 5 jul.2023.
url https://repositorio.ufrn.br/handle/123456789/54627
https://doi.org/10.1016/j.fufo.2020.100001
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