Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/23137 |
Resumo: | The dopamine transporter (DAT) is a major regulator of synaptic dopamine (DA) availability. It plays key roles in motor control and motor learning, memory formation, and reward-seeking behavior, is a major target of cocaine and methamphetamines, and has been assumed to be conserved among vertebrates. We have found, however, that birds, crocodiles, and lizards lack the DAT gene. We also found that the unprecedented loss of this important gene is compensated for by the expression of the noradrenaline transporter (NAT) gene, and not the serotonin transporter genes, in dopaminergic cells, which explains the peculiar pharmacology of the DA reuptake activity previously noted in bird striatum. This unexpected pattern contrasts with that of ancestral vertebrates (e.g. fish) and mammals, where the NAT gene is selectively expressed in noradrenergic cells. DA circuits in birds/reptiles and mammals thus operate with an analogous reuptake mechanism exerted by different genes, bringing new insights into gene expression regulation in dopaminergic cells and the evolution of a key molecular player in reward and addiction pathways. |
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Lovell, P. V.Kasimi, B.Carleton, J.Velho, Tarciso André FerreiraMello, C. V.2017-05-26T18:29:24Z2017-05-26T18:29:24Z2015-09-14https://repositorio.ufrn.br/jspui/handle/123456789/2313710.1038/srep14093engdopamine transportersynaptic dopaminenoradrenaline transporterdopaminergic cellsLiving without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe dopamine transporter (DAT) is a major regulator of synaptic dopamine (DA) availability. It plays key roles in motor control and motor learning, memory formation, and reward-seeking behavior, is a major target of cocaine and methamphetamines, and has been assumed to be conserved among vertebrates. We have found, however, that birds, crocodiles, and lizards lack the DAT gene. We also found that the unprecedented loss of this important gene is compensated for by the expression of the noradrenaline transporter (NAT) gene, and not the serotonin transporter genes, in dopaminergic cells, which explains the peculiar pharmacology of the DA reuptake activity previously noted in bird striatum. This unexpected pattern contrasts with that of ancestral vertebrates (e.g. fish) and mammals, where the NAT gene is selectively expressed in noradrenergic cells. DA circuits in birds/reptiles and mammals thus operate with an analogous reuptake mechanism exerted by different genes, bringing new insights into gene expression regulation in dopaminergic cells and the evolution of a key molecular player in reward and addiction pathways.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALLiving without DAT Loss and compensation of the dopamine transporter gene in sauropsids.pdfLiving without DAT Loss and compensation of the dopamine transporter gene in sauropsids.pdfTarcisoVelho_ICe_Living without DAT_2015application/pdf1280749https://repositorio.ufrn.br/bitstream/123456789/23137/1/Living%20without%20DAT%20Loss%20and%20compensation%20of%20the%20dopamine%20transporter%20gene%20in%20sauropsids.pdf087af8dce3b38e27f437cbb49176ebd8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/23137/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTLiving without DAT Loss and compensation of the dopamine transporter gene in sauropsids.pdf.txtLiving without DAT Loss and compensation of the dopamine transporter gene in sauropsids.pdf.txtExtracted texttext/plain63629https://repositorio.ufrn.br/bitstream/123456789/23137/5/Living%20without%20DAT%20Loss%20and%20compensation%20of%20the%20dopamine%20transporter%20gene%20in%20sauropsids.pdf.txt4963697203390aa7d69937910bbed840MD55THUMBNAILLiving without DAT Loss and compensation of the dopamine transporter gene in sauropsids.pdf.jpgLiving without DAT Loss and compensation of the dopamine transporter gene in sauropsids.pdf.jpgIM Thumbnailimage/jpeg9516https://repositorio.ufrn.br/bitstream/123456789/23137/6/Living%20without%20DAT%20Loss%20and%20compensation%20of%20the%20dopamine%20transporter%20gene%20in%20sauropsids.pdf.jpgf8b2b498faf4ae89297b42425a2be547MD56123456789/231372021-07-12 15:39:52.431oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-12T18:39:52Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
title |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
spellingShingle |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) Lovell, P. V. dopamine transporter synaptic dopamine noradrenaline transporter dopaminergic cells |
title_short |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
title_full |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
title_fullStr |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
title_full_unstemmed |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
title_sort |
Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles) |
author |
Lovell, P. V. |
author_facet |
Lovell, P. V. Kasimi, B. Carleton, J. Velho, Tarciso André Ferreira Mello, C. V. |
author_role |
author |
author2 |
Kasimi, B. Carleton, J. Velho, Tarciso André Ferreira Mello, C. V. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lovell, P. V. Kasimi, B. Carleton, J. Velho, Tarciso André Ferreira Mello, C. V. |
dc.subject.por.fl_str_mv |
dopamine transporter synaptic dopamine noradrenaline transporter dopaminergic cells |
topic |
dopamine transporter synaptic dopamine noradrenaline transporter dopaminergic cells |
description |
The dopamine transporter (DAT) is a major regulator of synaptic dopamine (DA) availability. It plays key roles in motor control and motor learning, memory formation, and reward-seeking behavior, is a major target of cocaine and methamphetamines, and has been assumed to be conserved among vertebrates. We have found, however, that birds, crocodiles, and lizards lack the DAT gene. We also found that the unprecedented loss of this important gene is compensated for by the expression of the noradrenaline transporter (NAT) gene, and not the serotonin transporter genes, in dopaminergic cells, which explains the peculiar pharmacology of the DA reuptake activity previously noted in bird striatum. This unexpected pattern contrasts with that of ancestral vertebrates (e.g. fish) and mammals, where the NAT gene is selectively expressed in noradrenergic cells. DA circuits in birds/reptiles and mammals thus operate with an analogous reuptake mechanism exerted by different genes, bringing new insights into gene expression regulation in dopaminergic cells and the evolution of a key molecular player in reward and addiction pathways. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-09-14 |
dc.date.accessioned.fl_str_mv |
2017-05-26T18:29:24Z |
dc.date.available.fl_str_mv |
2017-05-26T18:29:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/23137 |
dc.identifier.doi.none.fl_str_mv |
10.1038/srep14093 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/23137 |
identifier_str_mv |
10.1038/srep14093 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
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