Utilização de choques osmóticos sucessivos para encapsulação de curcumina e fisetina em células de levedura (Saccharomyces cerevisiae)

• Autor(a) principal: Medeiros, Fábio Gonçalves Macêdo de
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/38803
• topic: Osmoporação; Osmoporation; Curcumina; Curcumin; Fisetina; Fisetin; Microencapsulação; Microencapsulation; S. cerevisiae; S. cerevisiae; Engenharias.

Microencapsulation techniques have been studied as alternatives for increasing bioavailability of molecules with possible industrial applications, such as curcumin and fisetin, known for their anti-inflammatory and antioxidant properties. Their limited applications are related with their hydrophobic nature, which account for low solubility in biocompatible matrixes and reduced bioavailability. Therefore, this study aimed to evaluate the effect of using multiple osmotic shocks for microencapsulation of curcumin and fisetin into yeast cells (Saccharomyces cerevisiae), as well as the impact of these sequential osmotic treatments on cell viability. S. cerevisiae cells were subjected to osmoporation protocols, under glycerol (Generally Recognised As Safe, GRAS) aqueous solutions with the studied molecules. Additionally, supernatant was re-used for sequential osmoporation steps, aiming to increase encapsulation efficiency (%EE) and incorporated content (CI or FI). Quantification was carried through direct extraction, with ultrasonic bath and UV-Vis spectroscopy. Carrying three sequential osmotic shocks significantly increased (p < 0.05) both molecules’ %EE (from 13.89±0.79% to 33.13±0.88% for curcumin, and from 29.67±0.96% to 49.40±0.14% for fisetin) and internalized content (from 0.418±0.028 mg to 0.781±0.021 mg for CI, and from 0.755±0.016 mg to 0.895±0.009 mg for FI). Yeast viability, on the other hand, was significantly reduced, down to 15.56±1.55% on the third osmotic shock. Hence, it is possible to suggest that the application of two sequential osmotic shocks can have a positive impact on increasing %EE and encapsulated content, but cell viability is limiting when it comes to the third osmotic shock.