Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross

Detalhes bibliográficos
Autor(a) principal: Santos, Videanny Videnov Alves dos
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/12535
Resumo: The intake of adequate quantities of food, including those rich in vitamins, is necessary for a healthy life. The lack of vitamin A has been characterized as a public health problem in developing countries, however, a high intake of vitamin A can result in toxic and teratogenics effects. High concentrations of vitamin A have been observed in the livers of animals. The objective of this study was to assess the levels of retinol in chicken livers and verify the effect of frozen storage on these levels. 64 livers from two chicken strains, Cobb and Ross, were used, came from four different farms. We examined 32 livers from each strain, 8 samples from each farm. Liver sample were homogenized individually, then 4 aliquots were taken from each sample. One of aliquots was analyzed immediately after slaughter (T0), the others were analyzed after 30, 60 and 90 days of storage at -18oC (T30, T60 and T90, respectively). Retinol dosage in the liver was determined by High Performance Liquid Chromatography (HPLC). The levels of retinol varied significantly according to the strain. The mean retinol value in the fresh samples was 6678.0 ± 1337.7 and 8324.1 ± 1158.5 µg/100g in the Cobb and Ross strain, respectively. Values of 4258 ± 918.7 ± 1391.7 and 4650.5 ± 1391.7 μg/100g were found after 90 days of storage for Cobb and Ross strain, respectively. The liver freezing caused a significant reduction in their levels of retinol, causing a loss of up to 44% with respect to fresh livers. The reduction in retinol levels occurred from 30 days of storage. Even with the losses from the frozen, the ingestion of a typical portion of 100 g of liver, regardless the chicken strain analyzed, surpass all recommendations of consumption and the maximum tolerable intake of vitamin A (3000 μg/day) for adults
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spelling Santos, Videanny Videnov Alves doshttp://lattes.cnpq.br/1707786122676509http://lattes.cnpq.br/8520928220989866Maria, Carlos Alberto Bastos dehttp://lattes.cnpq.br/7290053828612504Correia, Roberta Targino Pintohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798196E6Dimenstein, Roberto2014-12-17T14:03:28Z2009-02-202014-12-17T14:03:28Z2008-09-29SANTOS, Videanny Videnov Alves dos. Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross. 2008. 80 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2008.https://repositorio.ufrn.br/jspui/handle/123456789/12535The intake of adequate quantities of food, including those rich in vitamins, is necessary for a healthy life. The lack of vitamin A has been characterized as a public health problem in developing countries, however, a high intake of vitamin A can result in toxic and teratogenics effects. High concentrations of vitamin A have been observed in the livers of animals. The objective of this study was to assess the levels of retinol in chicken livers and verify the effect of frozen storage on these levels. 64 livers from two chicken strains, Cobb and Ross, were used, came from four different farms. We examined 32 livers from each strain, 8 samples from each farm. Liver sample were homogenized individually, then 4 aliquots were taken from each sample. One of aliquots was analyzed immediately after slaughter (T0), the others were analyzed after 30, 60 and 90 days of storage at -18oC (T30, T60 and T90, respectively). Retinol dosage in the liver was determined by High Performance Liquid Chromatography (HPLC). The levels of retinol varied significantly according to the strain. The mean retinol value in the fresh samples was 6678.0 ± 1337.7 and 8324.1 ± 1158.5 µg/100g in the Cobb and Ross strain, respectively. Values of 4258 ± 918.7 ± 1391.7 and 4650.5 ± 1391.7 μg/100g were found after 90 days of storage for Cobb and Ross strain, respectively. The liver freezing caused a significant reduction in their levels of retinol, causing a loss of up to 44% with respect to fresh livers. The reduction in retinol levels occurred from 30 days of storage. Even with the losses from the frozen, the ingestion of a typical portion of 100 g of liver, regardless the chicken strain analyzed, surpass all recommendations of consumption and the maximum tolerable intake of vitamin A (3000 μg/day) for adultsA ingestão de quantidades adequadas de alimentos, incluindo aqueles ricos em vitaminas, é necessária para uma vida saudável. A carência de vitamina A tem sido caracterizada como um problema de saúde pública nos países em desenvolvimento, entretanto, uma alta ingestão de vitamina A pode resultar em efeitos tóxicos e teratogênicos. Altas concentrações de vitamina A tem sido observadas nos fígados de animais. O objetivo deste trabalho foi avaliar os níveis de retinol em fígados de frango e verificar o efeito da estocagem sob condições de congelamento sobre esses níveis. Foram utilizados 64 fígados de duas linhagens de frango, Cobb e Ross, provenientes de quatro diferentes propriedades. Examinamos 32 fígados de cada linhagem, 8 amostras de cada granja. As amostras de fígado foram homogeneizadas individualmente, em seguida foram retiradas de cada amostra 4 alíquotas. Uma das alíquotas foi analisada imediatamente após o abate (T0), as demais foram analisadas após 30, 60 e 90 dias de armazenamento a 18oC (T30, T60 e T90, respectivamente). A dosagem de retinol no fígado foi realizada através da Cromatografia Líquida de Alta Eficiência (CLAE). Os níveis de retinol variaram significativamente de acordo com a linhagem. O valor médio de retinol nas amostras frescas da linhagem Cobb e Ross foi de 6678,0 ± 1337,4 e 8324,1 ± 1158,5 µg/100g, respectivamente. Valores de 4258 ± 918,7 e 4650,5 ± 1391,7 μg/100g foram encontrados após 90 dias de estocagem para a linhagem Cobb e Ross, respectivamente. O congelamento do fígado causou uma redução significativa nos seus níveis de retinol, ocasionando uma perda de até 44% com relação aos fígados frescos. A redução nos níveis de retinol ocorreu a partir de 30 dias de estocagem. Mesmo com as perdas decorrentes do congelamento, a ingestão de uma típica porção de 100 g de fígado, independentemente da linhagem do frango analisada, ultrapassa todas as recomendações de consumo e o limite máximo de ingestão tolerável de vitamina A (3000 µg/dia) para adultosapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em BioquímicaUFRNBRBioquímica; Biologia MolecularFrangoFígadoRetinolCongelamentoCLAEChickenLiverRetinolFreezingHPLCCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Rossinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTVideannyVAS.pdf.txtVideannyVAS.pdf.txtExtracted texttext/plain103800https://repositorio.ufrn.br/bitstream/123456789/12535/6/VideannyVAS.pdf.txt54e29b330bc915fd3d8cc85d13b51198MD56AvaliacaoConcentracaoRetinol_Santos_2008.pdf.txtAvaliacaoConcentracaoRetinol_Santos_2008.pdf.txtExtracted texttext/plain103800https://repositorio.ufrn.br/bitstream/123456789/12535/8/AvaliacaoConcentracaoRetinol_Santos_2008.pdf.txt54e29b330bc915fd3d8cc85d13b51198MD58THUMBNAILVideannyVAS.pdf.jpgVideannyVAS.pdf.jpgIM Thumbnailimage/jpeg3328https://repositorio.ufrn.br/bitstream/123456789/12535/7/VideannyVAS.pdf.jpgf791d1d83eb42d48135748c88474b898MD57AvaliacaoConcentracaoRetinol_Santos_2008.pdf.jpgAvaliacaoConcentracaoRetinol_Santos_2008.pdf.jpgIM Thumbnailimage/jpeg3328https://repositorio.ufrn.br/bitstream/123456789/12535/9/AvaliacaoConcentracaoRetinol_Santos_2008.pdf.jpgf791d1d83eb42d48135748c88474b898MD59ORIGINALAvaliacaoConcentracaoRetinol_Santos_2008.pdfapplication/pdf505722https://repositorio.ufrn.br/bitstream/123456789/12535/1/AvaliacaoConcentracaoRetinol_Santos_2008.pdf93cf2e382da9c35e84a47a279a6d117fMD51123456789/125352019-01-29 16:46:50.141oai:https://repositorio.ufrn.br:123456789/12535Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2019-01-29T19:46:50Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.por.fl_str_mv Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
title Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
spellingShingle Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
Santos, Videanny Videnov Alves dos
Frango
Fígado
Retinol
Congelamento
CLAE
Chicken
Liver
Retinol
Freezing
HPLC
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
title_full Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
title_fullStr Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
title_full_unstemmed Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
title_sort Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross
author Santos, Videanny Videnov Alves dos
author_facet Santos, Videanny Videnov Alves dos
author_role author
dc.contributor.authorID.por.fl_str_mv
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/1707786122676509
dc.contributor.advisorID.por.fl_str_mv
dc.contributor.advisorLattes.por.fl_str_mv http://lattes.cnpq.br/8520928220989866
dc.contributor.referees1.pt_BR.fl_str_mv Maria, Carlos Alberto Bastos de
dc.contributor.referees1ID.por.fl_str_mv
dc.contributor.referees1Lattes.por.fl_str_mv http://lattes.cnpq.br/7290053828612504
dc.contributor.referees2.pt_BR.fl_str_mv Correia, Roberta Targino Pinto
dc.contributor.referees2ID.por.fl_str_mv
dc.contributor.referees2Lattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798196E6
dc.contributor.author.fl_str_mv Santos, Videanny Videnov Alves dos
dc.contributor.advisor1.fl_str_mv Dimenstein, Roberto
contributor_str_mv Dimenstein, Roberto
dc.subject.por.fl_str_mv Frango
Fígado
Retinol
Congelamento
CLAE
topic Frango
Fígado
Retinol
Congelamento
CLAE
Chicken
Liver
Retinol
Freezing
HPLC
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.eng.fl_str_mv Chicken
Liver
Retinol
Freezing
HPLC
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description The intake of adequate quantities of food, including those rich in vitamins, is necessary for a healthy life. The lack of vitamin A has been characterized as a public health problem in developing countries, however, a high intake of vitamin A can result in toxic and teratogenics effects. High concentrations of vitamin A have been observed in the livers of animals. The objective of this study was to assess the levels of retinol in chicken livers and verify the effect of frozen storage on these levels. 64 livers from two chicken strains, Cobb and Ross, were used, came from four different farms. We examined 32 livers from each strain, 8 samples from each farm. Liver sample were homogenized individually, then 4 aliquots were taken from each sample. One of aliquots was analyzed immediately after slaughter (T0), the others were analyzed after 30, 60 and 90 days of storage at -18oC (T30, T60 and T90, respectively). Retinol dosage in the liver was determined by High Performance Liquid Chromatography (HPLC). The levels of retinol varied significantly according to the strain. The mean retinol value in the fresh samples was 6678.0 ± 1337.7 and 8324.1 ± 1158.5 µg/100g in the Cobb and Ross strain, respectively. Values of 4258 ± 918.7 ± 1391.7 and 4650.5 ± 1391.7 μg/100g were found after 90 days of storage for Cobb and Ross strain, respectively. The liver freezing caused a significant reduction in their levels of retinol, causing a loss of up to 44% with respect to fresh livers. The reduction in retinol levels occurred from 30 days of storage. Even with the losses from the frozen, the ingestion of a typical portion of 100 g of liver, regardless the chicken strain analyzed, surpass all recommendations of consumption and the maximum tolerable intake of vitamin A (3000 μg/day) for adults
publishDate 2008
dc.date.issued.fl_str_mv 2008-09-29
dc.date.available.fl_str_mv 2009-02-20
2014-12-17T14:03:28Z
dc.date.accessioned.fl_str_mv 2014-12-17T14:03:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Videanny Videnov Alves dos. Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross. 2008. 80 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2008.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/12535
identifier_str_mv SANTOS, Videanny Videnov Alves dos. Avaliação da concentração de retinol em fígados frescos e congelados de frangos das linhagens Cobb e Ross. 2008. 80 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2008.
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