Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae

Detalhes bibliográficos
Autor(a) principal: Fidelis, Gabriel Pereira
Data de Publicação: 2014
Outros Autores: Câmara, Rafael Barros Gomes da, Queiroz, Moacir Fernandes, Costa, Mariana Santana Santos Pereira, Santos, Pablo Castro, Rocha, Hugo Alexandre de Oliveira, Costa, Leandro Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/31315
Resumo: The sulfated polysaccharides (SP) from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1)-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g) increased in the following order: GB2sp > GB1sp > GB2s > GB1s > GB1. However, the amount of SP extracted increased in a different way: GB2sp > GB1 > GB1sp > GB1s > GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT) test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT) test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp > GB2sp > GB1 > GB1s > GB2s. The total capacity antioxidant (TCA) of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiae
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spelling Fidelis, Gabriel PereiraCâmara, Rafael Barros Gomes daQueiroz, Moacir FernandesCosta, Mariana Santana Santos PereiraSantos, Pablo CastroRocha, Hugo Alexandre de OliveiraCosta, Leandro Silva2021-01-28T17:48:31Z2021-01-28T17:48:31Z2014-11-13FIDELIS, Gabriel Pereira; CAMARA, Rafael Barros Gomes da; QUEIROZ, Moacir Fernandes; COSTA, Mariana Santana Santos Pereira; SANTOS, Pablo Castro; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules, [s. l.], v. 19, n. 11, p. 18511-18526, 13 nov. 2014. MDPI AG. Disponível em: https://www.mdpi.com/1420-3049/19/11/18511. Acesso em: 05 ago. 2020. Http://dx.doi.org/10.3390/molecules191118511.1420-3049 (online)https://repositorio.ufrn.br/handle/123456789/3131510.3390/molecules191118511MDPIAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessAPTT assayTotal capacity antioxidantSonicationProteolysisRed seaweedProteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe sulfated polysaccharides (SP) from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1)-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g) increased in the following order: GB2sp > GB1sp > GB2s > GB1s > GB1. However, the amount of SP extracted increased in a different way: GB2sp > GB1 > GB1sp > GB1s > GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT) test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT) test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp > GB2sp > GB1 > GB1s > GB2s. The total capacity antioxidant (TCA) of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiaeengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALProteolysisNaohUltrasound_Câmara_2014.pdfProteolysisNaohUltrasound_Câmara_2014.pdfapplication/pdf681720https://repositorio.ufrn.br/bitstream/123456789/31315/1/ProteolysisNaohUltrasound_C%c3%a2mara_2014.pdfcf66bdad9f83d0d17fc183f184b3ca70MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/31315/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/31315/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTProteolysisNaohUltrasound_Câmara_2014.pdf.txtProteolysisNaohUltrasound_Câmara_2014.pdf.txtExtracted texttext/plain46295https://repositorio.ufrn.br/bitstream/123456789/31315/4/ProteolysisNaohUltrasound_C%c3%a2mara_2014.pdf.txt44f57beed19fc2b2bf789de58484b9d4MD54THUMBNAILProteolysisNaohUltrasound_Câmara_2014.pdf.jpgProteolysisNaohUltrasound_Câmara_2014.pdf.jpgGenerated Thumbnailimage/jpeg1656https://repositorio.ufrn.br/bitstream/123456789/31315/5/ProteolysisNaohUltrasound_C%c3%a2mara_2014.pdf.jpg0e634c5efb287e1314aba475c316e8d5MD55123456789/313152021-01-31 05:19:54.417oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-01-31T08:19:54Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
title Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
spellingShingle Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
Fidelis, Gabriel Pereira
APTT assay
Total capacity antioxidant
Sonication
Proteolysis
Red seaweed
title_short Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
title_full Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
title_fullStr Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
title_full_unstemmed Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
title_sort Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
author Fidelis, Gabriel Pereira
author_facet Fidelis, Gabriel Pereira
Câmara, Rafael Barros Gomes da
Queiroz, Moacir Fernandes
Costa, Mariana Santana Santos Pereira
Santos, Pablo Castro
Rocha, Hugo Alexandre de Oliveira
Costa, Leandro Silva
author_role author
author2 Câmara, Rafael Barros Gomes da
Queiroz, Moacir Fernandes
Costa, Mariana Santana Santos Pereira
Santos, Pablo Castro
Rocha, Hugo Alexandre de Oliveira
Costa, Leandro Silva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fidelis, Gabriel Pereira
Câmara, Rafael Barros Gomes da
Queiroz, Moacir Fernandes
Costa, Mariana Santana Santos Pereira
Santos, Pablo Castro
Rocha, Hugo Alexandre de Oliveira
Costa, Leandro Silva
dc.subject.por.fl_str_mv APTT assay
Total capacity antioxidant
Sonication
Proteolysis
Red seaweed
topic APTT assay
Total capacity antioxidant
Sonication
Proteolysis
Red seaweed
description The sulfated polysaccharides (SP) from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1)-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g) increased in the following order: GB2sp > GB1sp > GB2s > GB1s > GB1. However, the amount of SP extracted increased in a different way: GB2sp > GB1 > GB1sp > GB1s > GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT) test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT) test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp > GB2sp > GB1 > GB1s > GB2s. The total capacity antioxidant (TCA) of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiae
publishDate 2014
dc.date.issued.fl_str_mv 2014-11-13
dc.date.accessioned.fl_str_mv 2021-01-28T17:48:31Z
dc.date.available.fl_str_mv 2021-01-28T17:48:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FIDELIS, Gabriel Pereira; CAMARA, Rafael Barros Gomes da; QUEIROZ, Moacir Fernandes; COSTA, Mariana Santana Santos Pereira; SANTOS, Pablo Castro; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules, [s. l.], v. 19, n. 11, p. 18511-18526, 13 nov. 2014. MDPI AG. Disponível em: https://www.mdpi.com/1420-3049/19/11/18511. Acesso em: 05 ago. 2020. Http://dx.doi.org/10.3390/molecules191118511.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/31315
dc.identifier.issn.none.fl_str_mv 1420-3049 (online)
dc.identifier.doi.none.fl_str_mv 10.3390/molecules191118511
identifier_str_mv FIDELIS, Gabriel Pereira; CAMARA, Rafael Barros Gomes da; QUEIROZ, Moacir Fernandes; COSTA, Mariana Santana Santos Pereira; SANTOS, Pablo Castro; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules, [s. l.], v. 19, n. 11, p. 18511-18526, 13 nov. 2014. MDPI AG. Disponível em: https://www.mdpi.com/1420-3049/19/11/18511. Acesso em: 05 ago. 2020. Http://dx.doi.org/10.3390/molecules191118511.
1420-3049 (online)
10.3390/molecules191118511
url https://repositorio.ufrn.br/handle/123456789/31315
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
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instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
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