Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/tede/265 |
Resumo: | To verify the biochemical and physiological mechanisms involved in the nitrogen efficiency usage in rice, two rice varieties, Piau? (traditional from Maranh?o State) and IAC 47 (breed), were cultivated in growth chambers, in hydroponics system, and submitted to different levels of N-NO3 -. As a study strategy the plants were cultivated for eighteen days, counted after germination, in a basic media, submitted for 72 hours without nitrogen, and them they were divided in three groups and applied the treatments. The first received 0.15 mM, a second group received 5 mM of N-NO3 - while a third group stayed without nitrogen. After treatment application, plants were harvested at 0, 1, 3, 6, 9, 24 and 48 hours, and samples of shoots and root were taken for determination of nitrate, ammonium-N, amino-N soluble sugars and gene expression of the enzymes nitrate redutase (NADH-NR) and glutamine synthetase isoforms (GS) (GS1.1, GS1.2, GS2, GS2c, GSI) at 0, 6 and 24 hours. The Piau? variety, after 72 hours without N, still kept small NO3 - levels. Also, the variety Piau? presented a higher expression of OsGS1.1 gene, which suggests the participation of this enzyme in the control of internal N fluxes, when in absence of nitrogen in the external solution. Under high N level, the Piau? variety accumulated larger amounts of amino-N in the leaves, had small nitrate redutase expression and activity. In spite of no differences in glutamine synthetase activity, an increase in OsGS2 (GS2) expression was observed, suggesting that this is the main GS isoform responsible for the GS activity in rice plants under nitrate nutrition. |
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Fernandes, Manlio Silvestre002.180.573-34Souza, Sonia Regina de003.927.487-03http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784228Z9055.869.347-44Bucher, Carlos Alberto2016-04-26T19:36:54Z2009-03-092007-03-01BUCHER, Carlos Alberto. Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz. 2007. 50 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007.https://tede.ufrrj.br/jspui/handle/tede/265To verify the biochemical and physiological mechanisms involved in the nitrogen efficiency usage in rice, two rice varieties, Piau? (traditional from Maranh?o State) and IAC 47 (breed), were cultivated in growth chambers, in hydroponics system, and submitted to different levels of N-NO3 -. As a study strategy the plants were cultivated for eighteen days, counted after germination, in a basic media, submitted for 72 hours without nitrogen, and them they were divided in three groups and applied the treatments. The first received 0.15 mM, a second group received 5 mM of N-NO3 - while a third group stayed without nitrogen. After treatment application, plants were harvested at 0, 1, 3, 6, 9, 24 and 48 hours, and samples of shoots and root were taken for determination of nitrate, ammonium-N, amino-N soluble sugars and gene expression of the enzymes nitrate redutase (NADH-NR) and glutamine synthetase isoforms (GS) (GS1.1, GS1.2, GS2, GS2c, GSI) at 0, 6 and 24 hours. The Piau? variety, after 72 hours without N, still kept small NO3 - levels. Also, the variety Piau? presented a higher expression of OsGS1.1 gene, which suggests the participation of this enzyme in the control of internal N fluxes, when in absence of nitrogen in the external solution. Under high N level, the Piau? variety accumulated larger amounts of amino-N in the leaves, had small nitrate redutase expression and activity. In spite of no differences in glutamine synthetase activity, an increase in OsGS2 (GS2) expression was observed, suggesting that this is the main GS isoform responsible for the GS activity in rice plants under nitrate nutrition.Para verificar os mecanismos bioqu?micos e fisiol?gicos respons?veis pela efici?ncia de uso de nitrog?nio em arroz, duas variedades de arroz, Piau? (tradicional do estado do Maranh?o) e IAC 47 (melhorada), foram cultivadas em c?maras de crescimento, em sistema hidrop?nico, e submetidas a diferentes n?veis de N-NO3 -. Como estrat?gia de trabalho cultivou-se as plantas por 18 dias, contados ap?s a germina??o, em meio b?sico, submeteu-as por 72 horas sem suplementa??o de N e depois foram divididas em 3 grupos e aplicado os tratamentos. O primeiro recebeu 0,15 mM de N-NO3 -, o segundo submetido a 5 mM de N-NO3 - e o terceiro sem adi??o de N. Depois de aplicados os tratamentos foram realizadas as coletas nos tempos 0, 1, 3, 6, 9, 24, 48 horas e amostras de parte a?rea e raiz foram coletadas para an?lise de nitrato, N-amino, am?nio, a??cares sol?veis e a express?o dos genes que codificam para a enzima nitrato redutase (NADH-NR) e isoformas da enzima glutamina sintetase (GS) (GS1.1, GS1.2, GS2, GS2c, GSI) nos tempos 0, 6, e 24 horas. A variedade Piau?, depois de cultivada por 72 horas sem N, manteve baixos teores de NO3 -. Estas tamb?m apresentaram maior express?o de OsGS1.1. Isto sugere a participa??o desta enzima no controle do fluxo interno de N, quando sob defici?ncia deste nutriente na solu??o externa. Sob alto n?vel de N, a variedade Piau? acumulou maiores teores de N-amino nas folhas, menor express?o e atividade da nitrato redutase. Tamb?m nestas plantas, n?o houve diferen?as na atividade da glutamina sintetase, entretanto, ocorreu aumento da express?o de OsGS2 (GS2), sugerindo ser este o principal gene que codifica a GS respons?vel pela atividade de GS em plantas de arroz sob nutri??o n?trica.Made available in DSpace on 2016-04-26T19:36:54Z (GMT). No. of bitstreams: 1 2007 - Carlos Alberto Bucher.pdf: 983054 bytes, checksum: b9e5ced201894f8acddc42d070487f12 (MD5) Previous issue date: 2007-03-01FAPERJ - Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do Rio de Janeiroapplication/pdfhttps://tede.ufrrj.br/retrieve/3848/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/18140/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/24484/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/30847/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/37190/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/43576/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/49969/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpghttps://tede.ufrrj.br/retrieve/56448/2007%20-%20Carlos%20Alberto%20Bucher.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de Agronomianitratovariedades tradicionaisnitrato redutaseGlutamina sintetasenitratelandracenitrate redutaseAgronomiaAvalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arrozEvaluation by RT-PCR of the expression of genes that codify for enzymes of nitrogen assimilation in rice varietiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTEXT2007 - Carlos Alberto Bucher.pdf.txt2007 - Carlos Alberto Bucher.pdf.txttext/plain110812http://localhost:8080/tede/bitstream/tede/265/16/2007+-+Carlos+Alberto+Bucher.pdf.txtba494967941bad2b834a68208a7d9a8cMD516ORIGINAL2007 - Carlos Alberto Bucher.pdf2007 - Carlos Alberto Bucher.pdfapplication/pdf983156http://localhost:8080/tede/bitstream/tede/265/3/2007+-+Carlos+Alberto+Bucher.pdf7c42a73d40bd25fc3c75fafe1d2739d3MD53THUMBNAIL2007 - Carlos Alberto Bucher.pdf.jpg2007 - Carlos Alberto Bucher.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/265/17/2007+-+Carlos+Alberto+Bucher.pdf.jpg8c1280dca4077478b5fad29883580c72MD517tede/2652022-06-13 11:03:16.395oai:localhost:tede/265Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-13T14:03:16Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
dc.title.alternative.eng.fl_str_mv |
Evaluation by RT-PCR of the expression of genes that codify for enzymes of nitrogen assimilation in rice varieties |
title |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
spellingShingle |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz Bucher, Carlos Alberto nitrato variedades tradicionais nitrato redutase Glutamina sintetase nitrate landrace nitrate redutase Agronomia |
title_short |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
title_full |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
title_fullStr |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
title_full_unstemmed |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
title_sort |
Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz |
author |
Bucher, Carlos Alberto |
author_facet |
Bucher, Carlos Alberto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Manlio Silvestre |
dc.contributor.advisor1ID.fl_str_mv |
002.180.573-34 |
dc.contributor.advisor-co1.fl_str_mv |
Souza, Sonia Regina de |
dc.contributor.advisor-co1ID.fl_str_mv |
003.927.487-03 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784228Z9 |
dc.contributor.authorID.fl_str_mv |
055.869.347-44 |
dc.contributor.author.fl_str_mv |
Bucher, Carlos Alberto |
contributor_str_mv |
Fernandes, Manlio Silvestre Souza, Sonia Regina de |
dc.subject.por.fl_str_mv |
nitrato variedades tradicionais nitrato redutase Glutamina sintetase |
topic |
nitrato variedades tradicionais nitrato redutase Glutamina sintetase nitrate landrace nitrate redutase Agronomia |
dc.subject.eng.fl_str_mv |
nitrate landrace nitrate redutase |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
To verify the biochemical and physiological mechanisms involved in the nitrogen efficiency usage in rice, two rice varieties, Piau? (traditional from Maranh?o State) and IAC 47 (breed), were cultivated in growth chambers, in hydroponics system, and submitted to different levels of N-NO3 -. As a study strategy the plants were cultivated for eighteen days, counted after germination, in a basic media, submitted for 72 hours without nitrogen, and them they were divided in three groups and applied the treatments. The first received 0.15 mM, a second group received 5 mM of N-NO3 - while a third group stayed without nitrogen. After treatment application, plants were harvested at 0, 1, 3, 6, 9, 24 and 48 hours, and samples of shoots and root were taken for determination of nitrate, ammonium-N, amino-N soluble sugars and gene expression of the enzymes nitrate redutase (NADH-NR) and glutamine synthetase isoforms (GS) (GS1.1, GS1.2, GS2, GS2c, GSI) at 0, 6 and 24 hours. The Piau? variety, after 72 hours without N, still kept small NO3 - levels. Also, the variety Piau? presented a higher expression of OsGS1.1 gene, which suggests the participation of this enzyme in the control of internal N fluxes, when in absence of nitrogen in the external solution. Under high N level, the Piau? variety accumulated larger amounts of amino-N in the leaves, had small nitrate redutase expression and activity. In spite of no differences in glutamine synthetase activity, an increase in OsGS2 (GS2) expression was observed, suggesting that this is the main GS isoform responsible for the GS activity in rice plants under nitrate nutrition. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-03-01 |
dc.date.available.fl_str_mv |
2009-03-09 |
dc.date.accessioned.fl_str_mv |
2016-04-26T19:36:54Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BUCHER, Carlos Alberto. Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz. 2007. 50 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/265 |
identifier_str_mv |
BUCHER, Carlos Alberto. Avalia??o atrav?s de RT-PCR da express?o dos genes que codificam para enzimas de assimila??o de nitrog?nio em variedades de arroz. 2007. 50 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
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Universidade Federal Rural do Rio de Janeiro |
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Universidade Federal Rural do Rio de Janeiro |
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