Variabilidade gen?tica e rea??o a doen?as em acessos de Capsicum baccatum

• Autor(a) principal: Martinez, Aur?lio Ludovico de Almeida
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/1986
• topic: Genetic resource; Genebank; Pepper; Molecular markers; Recursos gen?ticos; Cole??o de germoplasma; Pimenta; Marcadores moleculares; Agronomia

Originating from Americas, with Brazil as an important center of diversity, Capsicum occupies an important position among greenery. This study aimed to 1) to assess the genetic similarity and presence of duplicates; 2) to evaluate the resistance to anthracnose disease 3) screening viruses resistance in Capsicum baccatum accessions from Instituto Federal Goiano - Campus Ceres genebank. The genetic similarity was determined by molecular characterization at Embrapa Recursos Gen?ticos e Biotecnologia through RAPD and ISSR markers and morphological descriptors defined for Capsicum spp. According to the molecular and morphological characterization it was confirm the genetic diversity among C. baccatum accessions and the lack of duplicates. The anthracnose resistance was evaluated at 50 and 120 days after the dispersion of pepper powder prepared from infected peppers, considering incidence (presence or absence of lesions in fruits) and disease severity (surface damage percentage to the surface total of fruit) through images processing of diseased fruits. The essay was carried out under field conditions, using augmented randomized complete block design with fifteen plants per plot and three commercial varieties as control. Significant difference was observed especially in disease severity between treatments in both periods observed. The viruses incidence was evaluated in two field experiments in the periods between June-December 2012 and January-June 2013. DAS-ELISA test was performed with absorbance reading at 405 nm on a plate reader, all symptomatic and four asymptomatic plants in all plots was sampled, at 180 days after transplanting in the first experiment, and 160 days after transplanting in second one. The Viruses evaluated were tobamovirus Pepper mild motle virus (PMMoV), the cucumovirus Cucumber mosaic virus (CMV), the tospovirus Groundnut ring spot virus (GRSV), Tomato spot wilt virus (TSWV) and Tomato chlorotic spot virus (TCSV) and potyvirus Potato virus Y (PVY) and Pepper yellow mosaic virus (PepYMV). The percentage of incidence in the first and second period evaluated, were 30% e 7% PMMoV, 6% e 27% CMV, 8% e 12% PVY, 25% e 22% PepYMV, 10% e 2% GRSV, 11% e 0% TCSV, 0% e 11% TSWV. Virus were not detected several accessions studied and are potential sources of virus resistance that should be confirmed on resistance testing under controlled conditions.